Predictions for oxygen supply control to enhance population stability of engineered production strains.

abstract：:Cellulolytic fungi, such as Trichoderma reesei, T. lignorum, and Chaetomium cellulolyticum reach a low packing density of mycelia when grown on straw under conditions of solid substrate fermentation. The low packing density is shown to be caused partially by the geometric limitation of the growth of mycelia in the sub...

journal_title：Biotechnology and bioengineering

authors： Laukevics JJ,Apsite AF,Viesturs US,Tengerdy RP

更新日期：1985-12-01 00:00:00

abstract：:Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impai...

journal_title：Biotechnology and bioengineering

authors： Zhou K,Zou R,Zhang C,Stephanopoulos G,Too HP

更新日期：2013-09-01 00:00:00

abstract：:Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...

journal_title：Biotechnology and bioengineering

authors： Ritter A,Rauschert T,Oertli M,Piehlmaier D,Mantas P,Kuntzelmann G,Lageyre N,Brannetti B,Voedisch B,Geisse S,Jostock T,Laux H

更新日期：2016-11-01 00:00:00

abstract：:A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs-Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox-magnetohydrodynamics (redox-MHD) conditions. Redox-MHD possesses uni...

journal_title：Biotechnology and bioengineering

authors： Cheah LT,Fritsch I,Haswell SJ,Greenman J

更新日期：2012-07-01 00:00:00

abstract：:Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...

journal_title：Biotechnology and bioengineering

authors： Kober L,Zehe C,Bode J

更新日期：2012-10-01 00:00:00

abstract：:The damaging and degenerative effects in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and Crohn's disease often manifests as the formation of lesions that feature a high local concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). GM-CSF along with other pro-inflammatory fa...

journal_title：Biotechnology and bioengineering

authors： Mosabbir AA,Qudrat A,Truong K

更新日期：2018-04-01 00:00:00

abstract：:We investigated the role of green sulfur bacteria inlight-responsive electricity generation in microbial electrochemical cells (MXCs). We operated MXCs containing either monocultures or defined cocultures of previously enriched phototrophic Chlorobium and anode-respiring Geobacter under anaerobic conditions in the abs...

journal_title：Biotechnology and bioengineering

authors： Badalamenti JP,Torres CI,Krajmalnik-Brown R

更新日期：2014-02-01 00:00:00

abstract：:Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...

journal_title：Biotechnology and bioengineering

authors： Tokuyama K,Toya Y,Horinouchi T,Furusawa C,Matsuda F,Shimizu H

更新日期：2018-06-01 00:00:00

abstract：:The simultaneous biodegradation of the nonionic surfactant Tween 20 (Tw20) and pentachlorophenol (PCP) by Sphingomonas chlorophenolica sp. Strain RA2 (RA2) was measured. As a sole substrate, Tw20 biodegradation was best described by the Contois kinetic model. During concurrent biodegradation of Tw20 and PCP, the biode...

journal_title：Biotechnology and bioengineering

authors： Bielefeldt AR,Cort T

更新日期：2005-03-20 00:00:00

abstract：:It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

journal_title：Biotechnology and bioengineering

authors： Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

更新日期：2008-05-01 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...

journal_title：Biotechnology and bioengineering

authors： Agena SM,Pusey ML,Bogle ID

更新日期：1999-07-20 00:00:00

abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...

journal_title：Biotechnology and bioengineering

authors： Gianfreda L,Toscano G,Pirozzi D,Greco G Jr

更新日期：1991-12-05 00:00:00

abstract：:For clinical trials, large amounts of high-titer retroviral supernatants are required. However, retroviral concentration is relatively low compared with other viral vectors. Moreover, less than half of retroviral vectors suspended in a collected supernatant are infectious because of their short half-lives. In this stu...

journal_title：Biotechnology and bioengineering

authors： Kwon YJ,Peng CA

更新日期：2005-05-05 00:00:00

abstract：:The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

journal_title：Biotechnology and bioengineering

authors： Yousefpour P,Chilkoti A

更新日期：2014-09-01 00:00:00

abstract：:The biotransformation of benzaldehyde and glucose to L-phenylacetylcarbinol (PAC) using Candida utilis was demonstrated in a solid-liquid two-phase partitioning bioreactor (TPPB) with the aim of reducing substrate, product, and by-product toxicity via sequestration. Previous work in the field had used octanol as the s...

journal_title：Biotechnology and bioengineering

authors： Khan TR,Daugulis AJ

更新日期：2010-11-01 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limi...

journal_title：Biotechnology and bioengineering

authors： Schoborg JA,Hershewe JM,Stark JC,Kightlinger W,Kath JE,Jaroentomeechai T,Natarajan A,DeLisa MP,Jewett MC

更新日期：2018-03-01 00:00:00

abstract：:The effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) scaffolds with varying pore geometries (square and hexagonal channels) were investigated. The scaffold with sq...

journal_title：Biotechnology and bioengineering

authors： Aliabouzar M,Lee SJ,Zhou X,Zhang GL,Sarkar K

更新日期：2018-02-01 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...

journal_title：Biotechnology and bioengineering

authors： Colombié S,Tweddell RJ,Condoret JS,Marty A

更新日期：1998-11-05 00:00:00

abstract：:We designed a bioartificial liver support system in which encapsulated multicellular spheroids of rat hepatocytes were utilized as a bioreactor in a hollow fiber cartridge. The spheroids, formed in a positively charged polystyrene dish that contained hormonally defined medium, were encapsulated into microdroplets of a...

journal_title：Biotechnology and bioengineering

authors： Shiraha H,Koide N,Hada H,Ujike K,Nakamura M,Shinji T,Gotoh S,Tsuji T

更新日期：1996-05-20 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:The sustainability of autotrophic granular system performing partial nitritation and anaerobic ammonium oxidation (anammox) for complete nitrogen removal is impaired by the production of nitrous oxide (N2 O). A systematic analysis of the pathways and affecting parameters is, therefore, required for developing N 2 O mi...

journal_title：Biotechnology and bioengineering

authors： Chen X,Ni BJ,Sin G

更新日期：2019-06-01 00:00:00

abstract：:Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

journal_title：Biotechnology and bioengineering

authors： Kim Y,Arp DJ,Semprini L

更新日期：2002-12-05 00:00:00

abstract：:The engineering of production cell lines to express anti-apoptotic genes has been pursued in recent years due to potential process benefits, including enhanced cell survival, increased protein expression, and improved product quality. In this study, a baby hamster kidney cell line secreting recombinant factor VIII (BH...

journal_title：Biotechnology and bioengineering

authors： Nivitchanyong T,Martinez A,Ishaque A,Murphy JE,Konstantinov K,Betenbaugh MJ,Thrift J

更新日期：2007-11-01 00:00:00

abstract：:Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to re...

journal_title：Biotechnology and bioengineering

authors： Wu-Fienberg Y,Moore AM,Marquardt LM,Newton P,Johnson PJ,Mackinnon SE,Sakiyama-Elbert SE,Wood MD

更新日期：2014-09-01 00:00:00

abstract：:Fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (VOC). Fungi foster reduced transport limitation of hydrophobic VOCs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. Nev...

journal_title：Biotechnology and bioengineering

authors： Vergara-Fernández A,Hernández S,Revah S

更新日期：2011-04-01 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00

abstract：:The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) of transf...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Bae SW,Jeong Hong H,Min Lee G

更新日期：1996-08-20 00:00:00