Development of a novel ER stress based selection system for the isolation of highly productive clones.

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:Steam explosion after sulphur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there...

journal_title：Biotechnology and bioengineering

authors： Wong KK,Deverell KF,Mackie KL,Clark TA,Donaldson LA

更新日期：1988-04-05 00:00:00

abstract：:A modified simulation procedure based on a statistical approach was investigated. The procedure predicts the time course of fed-batch culture for glutamic acid production by a temperature-sensitive strain of Brevibacterium flavum. The statistical approach requires only a data base of state points obtained in experimen...

journal_title：Biotechnology and bioengineering

authors： Kishimoto M,Alfafara CG,Nakajima M,Yoshida T,Taguchi H

更新日期：1989-01-10 00:00:00

abstract：:Biomanufacturing exhibits inherent variability that can lead to variation in performance attributes and batch failure. To help ensure process consistency and product quality the development of predictive models and integrated control strategies is a promising approach. In this study, a feedback controller was develope...

journal_title：Biotechnology and bioengineering

authors： Schmitt J,Downey B,Beller J,Russell B,Quach A,Lyon D,Curran M,Mulukutla BC,Chu C

更新日期：2019-09-01 00:00:00

abstract：:The feasibility of continuous production of proteins in chemostat cultures of Bacillus subtilis was investigated. An expression system consisting of the bacterium B. subtilis BR151 carrying plasmid p602/19 was used. The plasmid contains the cat (chioramphenicol acetyltrans-ferase) gene downstream of a strong vegetativ...

journal_title：Biotechnology and bioengineering

authors： Vierheller C,Goel A,Peterson M,Domach MM,Ataai MM

更新日期：1995-09-05 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

journal_title：Biotechnology and bioengineering

authors： Minier M,Grateloup R,Blanc-Ferras E,Goma G

更新日期：1990-04-15 00:00:00

abstract：:Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...

journal_title：Biotechnology and bioengineering

authors： Landberg J,Wright NR,Wulff T,Herrgård MJ,Nielsen AT

更新日期：2020-08-18 00:00:00

abstract：:Hairy root cultures of Catharanthus roseus were established by infection of seedlings with Agrobacterium rhizogenes 15834. Approximately 150 transformants from four different. C. roseus cultivars were screened for desirable traits in growth and indole alkaloid production. Five hairy root clones grew well in liquid cul...

journal_title：Biotechnology and bioengineering

authors： Bhadra R,Vani S,Shanks JV

更新日期：1993-03-05 00:00:00

abstract：:The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...

journal_title：Biotechnology and bioengineering

authors： Kan E,Deshusses MA

更新日期：2009-02-15 00:00:00

abstract：:The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...

journal_title：Biotechnology and bioengineering

authors： Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg H

更新日期：1989-01-05 00:00:00

abstract：:The simultaneous growth and product formation in a microbial culture is an important feature of several laboratory, industrial, and environmental bioprocesses. Metabolic burden associated with product formation in these bioprocesses may lead to growth advantage of a nonproducing mutant leading to a loss of the produci...

journal_title：Biotechnology and bioengineering

authors： Varma A,Palsson BO

更新日期：1994-02-20 00:00:00

abstract：:Microbial degradation of trichloroethylene (TCE) has been demonstrated under aerobic conditions with propane. The primary objective of this research was to evaluate the feasibility of introducing a vapor phase form of TCE in the presence of propane to batch bioreactors containing a liquid phase suspension of Mycobacte...

journal_title：Biotechnology and bioengineering

authors： Wilcox DW,Autenrieth RL,Bonner JS

更新日期：1995-05-20 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

journal_title：Biotechnology and bioengineering

authors： Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

更新日期：2015-07-01 00:00:00

abstract：:Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...

journal_title：Biotechnology and bioengineering

authors： Criddle CS

更新日期：1993-05-01 00:00:00

abstract：:The engineering of production cell lines to express anti-apoptotic genes has been pursued in recent years due to potential process benefits, including enhanced cell survival, increased protein expression, and improved product quality. In this study, a baby hamster kidney cell line secreting recombinant factor VIII (BH...

journal_title：Biotechnology and bioengineering

authors： Nivitchanyong T,Martinez A,Ishaque A,Murphy JE,Konstantinov K,Betenbaugh MJ,Thrift J

更新日期：2007-11-01 00:00:00

abstract：:RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger RNA (mRNA) splicing, has thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclona...

journal_title：Biotechnology and bioengineering

authors： Tzani I,Monger C,Motheramgari K,Gallagher C,Hagan R,Kelly P,Costello A,Meiller J,Floris P,Zhang L,Clynes M,Bones J,Barron N,Clarke C

更新日期：2020-08-01 00:00:00

abstract：:Low-temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a ...

journal_title：Biotechnology and bioengineering

authors： Kawakami M,Koya H,Gondo S

更新日期：1988-07-20 00:00:00

abstract：:Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...

journal_title：Biotechnology and bioengineering

authors： Choi O,Das A,Yu CP,Hu Z

更新日期：2010-12-15 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:The enzymology and kinetics of tyrosine phenol lyase (TPL) from Erwinia herbicola, and tyrosine decarboxylase (TDC) from Streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. In this multienzyme biotransformation using whole c...

journal_title：Biotechnology and bioengineering

authors： Anderson WA,Moo-Young M,Legge RL

更新日期：1992-03-25 00:00:00

abstract：:The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

journal_title：Biotechnology and bioengineering

authors： Yousefpour P,Chilkoti A

更新日期：2014-09-01 00:00:00

abstract：:One potential advantage of an extremely thermophilic metabolic engineering host (T opt ≥ 70°C) is facilitated recovery of volatile chemicals from the vapor phase of an active fermenting culture. This process would reduce purification costs and concomitantly alleviate toxicity to the cells by continuously removing solv...

journal_title：Biotechnology and bioengineering

authors： Zeldes BM,Straub CT,Otten JK,Adams MWW,Kelly RM

更新日期：2018-12-01 00:00:00

abstract：:Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumu...

journal_title：Biotechnology and bioengineering

authors： Huang J,Pinder KL

更新日期：1995-02-05 00:00:00

abstract：:13C metabolic flux analysis (MFA) is based on carbon-labeling experiments where a specifically (13)C labeled substrate is fed. The labeled carbon atoms distribute over the metabolic network and the label enrichment of certain metabolic pools is measured by using different methods. Recently, MS methods have been dramat...

journal_title：Biotechnology and bioengineering

authors： Wahl SA,Dauner M,Wiechert W

更新日期：2004-02-05 00:00:00

abstract：:Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...

journal_title：Biotechnology and bioengineering

authors： Kim MH,Wang N,McDonald T,Chu KH

更新日期：2012-12-01 00:00:00

abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

journal_title：Biotechnology and bioengineering

authors： Moussy Y

更新日期：2000-04-20 00:00:00

abstract：:Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarc...

journal_title：Biotechnology and bioengineering

authors： Nordblad M,Adlercreutz P

更新日期：2008-04-15 00:00:00

abstract：:The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimE gene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the ta...

journal_title：Biotechnology and bioengineering

authors： Ham TS,Lee SK,Keasling JD,Arkin AP

更新日期：2006-05-05 00:00:00