Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.

abstract：:Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...

journal_title：Biotechnology and bioengineering

authors： Hoelzle RD,Virdis B,Batstone DJ

更新日期：2014-11-01 00:00:00

abstract：:The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Syn...

journal_title：Biotechnology and bioengineering

authors： Agostoni M,Waters CM,Montgomery BL

更新日期：2016-02-01 00:00:00

abstract：:Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrat...

journal_title：Biotechnology and bioengineering

authors： Parvaresh F,Robert H,Thomas D,Legoy MD

更新日期：1992-02-20 00:00:00

abstract：:Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations w...

journal_title：Biotechnology and bioengineering

authors： Acosta MA,Velasquez M,Williams K,Ross JM,Leach JB

更新日期：2012-10-01 00:00:00

abstract：:Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a s...

journal_title：Biotechnology and bioengineering

authors： Sucosky P,Osorio DF,Brown JB,Neitzel GP

更新日期：2004-01-05 00:00:00

abstract：:We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...

journal_title：Biotechnology and bioengineering

authors： Goudar CT

更新日期：2011-10-01 00:00:00

abstract：:The influence of the factors acetic acid, furfural, and p-hydroxybenzoic acid on the ethanol yield (YEtOH) of Saccharomyces cerevisiae, bakers' yeast, S. cerevisiae ATCC 96581, and Candida shehatae NJ 23 was investigated using a 2(3)-full factorial design with 3 centrepoints. The results indicated that acetic acid inh...

journal_title：Biotechnology and bioengineering

authors： Palmqvist E,Grage H,Meinander NQ,Hahn-Hägerdal B

更新日期：1999-04-05 00:00:00

abstract：:The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

journal_title：Biotechnology and bioengineering

authors： Beun JJ,Heijnen JJ,van Loosdrecht MC

更新日期：2001-10-05 00:00:00

abstract：:A general framework for a genetically structured model is presented. The framework allows description of the interactions in a system of regulatory and structural genes. The model assumes equilibrium kinetics for the binding of regulatory proteins to the promoter regions of the genes and includes the possible activati...

journal_title：Biotechnology and bioengineering

authors： Agger T,Nielsen J

更新日期：1999-01-01 00:00:00

abstract：:An experimental study was undertaken to quantify the effects of infection cell density, medium condition, and surface aeration on recombinant protein yields in insect cells. In the absence of surface aeration and fresh medium, insect cells generated higher product yields (on a per cell basis) when infected with recomb...

journal_title：Biotechnology and bioengineering

authors： Lindsay DA,Betenbaugh MJ

更新日期：1992-03-15 00:00:00

abstract：:Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

journal_title：Biotechnology and bioengineering

authors： Latulippe DR,Zydney AL

更新日期：2008-02-01 00:00:00

abstract：:Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that the mechanical environment can decisively influence the development of engineered tissues cultured in vitro. To investigate the effects of the hydrodynamic environment on tissue-engineered cartilage, primary bov...

journal_title：Biotechnology and bioengineering

authors： Gooch KJ,Kwon JH,Blunk T,Langer R,Freed LE,Vunjak-Novakovic G

更新日期：2001-02-20 00:00:00

abstract：:Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online d...

journal_title：Biotechnology and bioengineering

authors： Regestein L,Maskow T,Tack A,Knabben I,Wunderlich M,Lerchner J,Büchs J

更新日期：2013-05-01 00:00:00

abstract：:Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumu...

journal_title：Biotechnology and bioengineering

authors： Huang J,Pinder KL

更新日期：1995-02-05 00:00:00

abstract：:The effects of ZnCl2 additions on a mercuric reductase, merA, ammonia monooxygenase, amoA, and hydroxylamine (NH2OH) oxidoreductase, hao, gene expression were examined in continuously cultured Nitrosomonas europaea cells. The reactor was operated for 85 days with a 6.9 d hydraulic retention time and with four successi...

journal_title：Biotechnology and bioengineering

authors： Radniecki TS,Semprini L,Dolan ME

更新日期：2009-02-01 00:00:00

abstract：:Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...

journal_title：Biotechnology and bioengineering

authors： Peyton SR,Kalcioglu ZI,Cohen JC,Runkle AP,Van Vliet KJ,Lauffenburger DA,Griffith LG

更新日期：2011-05-01 00:00:00

abstract：:We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

journal_title：Biotechnology and bioengineering

authors： Martins JF,Da Ponte MN,Barreiros S

更新日期：1993-08-05 00:00:00

abstract：:Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical ...

journal_title：Biotechnology and bioengineering

authors： Tashima AK,Ottens M,Van der Wielen LA,Cintra DE,Pauli JR,Filho Pde A,Miranda EA

更新日期：2009-08-01 00:00:00

abstract：:Modifications are made to an earlier thermodynamic model (TEEM1) for prediction of maximum microbial yields from aerobic and anaerobic as well as heterotrophic and autotrophic growth. The revised model (TEEM2) corrects for lower yields found with aerobic oxidations of organic compounds where an oxygenase is involved a...

journal_title：Biotechnology and bioengineering

authors： McCarty PL

更新日期：2007-06-01 00:00:00

abstract：:Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...

journal_title：Biotechnology and bioengineering

authors： Meyer F,Gehmlich I,Guthke R,Górak A,Knorre WA

更新日期：1998-07-20 00:00:00

abstract：:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...

journal_title：Biotechnology and bioengineering

authors： Choi MH,Xu J,Rho JK,Shim JH,Yoon SC

更新日期：2009-03-01 00:00:00

abstract：:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...

journal_title：Biotechnology and bioengineering

authors： Xie S,Svec F,Fréchet JM

更新日期：1999-01-05 00:00:00

abstract：:A model that was used to describe toxicity from high concentrations of chlorinated aliphatic hydrocarbons (CAHs) on reductively dechlorinating cultures in batch reactors (Sabalowsky and Semprini (in press)) was extended here to simulate observations in continuous flow suspended and attached growth reactors. The reduct...

journal_title：Biotechnology and bioengineering

authors： Sabalowsky AR,Semprini L

更新日期：2010-10-15 00:00:00

abstract：:The removal of substate molecules from aerobic microbial cultures is due to both consumption by microorganisms and stripping by the air stream. The air stripping component can be described by a constant parameter for low concentrations of volatile substrates. This air stripping parameter was found to have a value of 0...

journal_title：Biotechnology and bioengineering

authors： Singh N,Hill GA

更新日期：1987-09-01 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the s...

journal_title：Biotechnology and bioengineering

authors： Giorno L,Li N,Drioli E

更新日期：2003-12-20 00:00:00

abstract：:Multivalent cations have been known to be important components of activated sludge floc structure due to their bridging ability of the negatively charged sites on the biopolymer network. Recently in batch systems it was found that excess concentration of monovalent cations led to the deterioration in settleability, de...

journal_title：Biotechnology and bioengineering

authors： Kara F,Gurakan GC,Sanin FD

更新日期：2008-06-01 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00

abstract：:Point and 95% confidence interval estimates of the true growth yield and maintenance coefficient are reported for four pure cultures andtwo mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus grown anaerobically on 3% nonfat dry milk using batch followed by fed batch culture. An improved method o...

journal_title：Biotechnology and bioengineering

authors： Oner MD,Erickson LE,Yang SS

更新日期：1986-06-01 00:00:00

abstract：:The kinetic properties of collagen-bound firefly luciferase have been investigated. Under definite hydrodynamic conditions with low agitation in the reaction medium, the observed behavior is modified compared to the enzyme free in solution: reducing the stirring rate decreases the observed enzymatic activity. But diff...

journal_title：Biotechnology and bioengineering

authors： Blum LJ,Coulet PR

更新日期：1986-08-01 00:00:00