Forecasting and control of lactate bifurcation in Chinese hamster ovary cell culture processes.

abstract：:A cyclic fed-batch bioprocess is designed and a significant improvement of rice alpha-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media...

journal_title：Biotechnology and bioengineering

authors： Chang CC,Ryu DD,Park CS,Kim JY

更新日期：1998-08-05 00:00:00

abstract：:The feasibility of producing ethanol in a continuous system from cellulose using Clostridirrrn thermocellum was investigated. This anaerobic and therniophilic bacterium was able to degrade cellulose directly into ethanol with acetic acid, hydrogen. and carbon dioxide as by-products of this fermentation. The fermentati...

journal_title：Biotechnology and bioengineering

authors： Zertuche L,Zall RR

更新日期：1982-01-01 00:00:00

abstract：:A titration bioassay, designed to accurately determine the activity of acetoclastic methanogens, is described that also allows evaluation of inhibition due to potential toxicants on the active biomass. The instrument is made of a pH-stat connected to an anaerobic batch reactor. Acetate is blended and mixed with anaero...

journal_title：Biotechnology and bioengineering

authors： Rozzi A,Castellazzi L,Speece RE

更新日期：2002-01-05 00:00:00

abstract：:Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...

journal_title：Biotechnology and bioengineering

authors： Souret FF,Kim Y,Wyslouzil BE,Wobbe KK,Weathers PJ

更新日期：2003-09-20 00:00:00

abstract：:Working conditions of a biochemical fuel cell formed by an oxygen cathode and a platinum bioanode in a Saccharomyces cerevisiae suspension metabolizing glucose are described. The biocell response in terms of bioanode potential and current drainage under different fermentation conditions is reported. A kinetic equation...

journal_title：Biotechnology and bioengineering

authors： Videla HA,Arvía AJ

更新日期：1975-10-01 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:Metabolic carbon labelling experiments enable a large amount of extracellular fluxes and intracellular carbon isotope enrichments to be measured. Since the relation between the measured quantities and the unknown intracellular metabolic fluxes is given by bilinear balance equations, flux determination from this data s...

journal_title：Biotechnology and bioengineering

authors： Wiechert W,Siefke C,de Graaf AA,Marx A

更新日期：1997-07-05 00:00:00

abstract：:The photosynthetic performance of a helical tubular photobioreactor ("Biocoil"), incorporating the filamentous cyanobacterium Spirulina platensis, was investigated. The photobioreactor was constructed in a cylindrical shape (0.9 m high) with a 0.25-m(2)basal area and a photostage comprising 60 m of transparent PVC tub...

journal_title：Biotechnology and bioengineering

authors： Watanabe Y,de la Noüe J,Hall DO

更新日期：1995-07-20 00:00:00

abstract：:A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...

journal_title：Biotechnology and bioengineering

authors： Dermoun Z,Belaich JP

更新日期：1985-07-01 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:The yeast Pachysolen tannophilus was entrapped in calcium alginate beads to ferment D-xylose on a continous basis in the presence of high cell densities. Experimental operating variables included the feed D-xylose concentration, the dilution rate, and the fermentor biomass concentration. Under favorable operating cond...

journal_title：Biotechnology and bioengineering

authors： Slininger PJ,Bothast RJ,Black LT,McGhee JE

更新日期：1982-10-01 00:00:00

abstract：:Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions ...

journal_title：Biotechnology and bioengineering

authors： Dismer F,Hansen S,Oelmeier SA,Hubbuch J

更新日期：2013-03-01 00:00:00

abstract：:In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...

journal_title：Biotechnology and bioengineering

authors： Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

更新日期：1989-09-01 00:00:00

abstract：:A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...

journal_title：Biotechnology and bioengineering

authors： Tiller JC,Lee SB,Lewis K,Klibanov AM

更新日期：2002-08-20 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00

abstract：:Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

journal_title：Biotechnology and bioengineering

authors： Gupta KG,Yadav NK,Dhawan S

更新日期：1978-12-01 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:Bioprocess identification starts with collection of process information. Usually there is a variety of information available, consisting of actual measurement data, historical data, empirical kinetic and yield correlations, and general knowledge available from literature. A central problem is to find out how the vario...

journal_title：Biotechnology and bioengineering

authors： Noorman HJ,Romein B,Luyben KC,Heijnen JJ

更新日期：1996-02-20 00:00:00

abstract：:High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...

journal_title：Biotechnology and bioengineering

authors： Chu IM,Papoutsakis ET

更新日期：1987-01-01 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:A starvation-based dissolved oxygen (DO) transient controller was developed to supply growth-limiting substrate to high cell density fed-batch cultures of recombinant Escherichia coli. The algorithm adjusted a preexisting feed rate in proportion to the culture's oxygen demand, which was estimated from transients in th...

journal_title：Biotechnology and bioengineering

authors： Whiffin VS,Cooney MJ,Cord-Ruwisch R

更新日期：2004-02-20 00:00:00

abstract：:The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...

journal_title：Biotechnology and bioengineering

authors： Wang YF,Masuda M,Tsujimura S,Kano K

更新日期：2008-10-15 00:00:00

abstract：:Non-autologous somatic gene therapy is an alternate approach to delivering recombinant gene products through implantation of a "universal" donor cell line engineered to produce a therapeutic gene product. The cells are immunologically isolated by enclosure in immunoprotective microcapsules fabricated from alginate-pol...

journal_title：Biotechnology and bioengineering

authors： Awrey DE,Tse M,Hortelano G,Chang PL

更新日期：1996-11-20 00:00:00

abstract：:Antimicrobial peptides (AMPs) kill microbes by non-specific membrane permeabilization, making them ideal templates for designing novel peptide-based antibiotics that can combat multi-drug resistant pathogens. For maximum efficacy in vivo and in vitro, AMPs must be biocompatible, salt-tolerant and possess broad-spectru...

journal_title：Biotechnology and bioengineering

authors： Saravanan R,Li X,Lim K,Mohanram H,Peng L,Mishra B,Basu A,Lee JM,Bhattacharjya S,Leong SS

更新日期：2014-01-01 00:00:00

abstract：:A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...

journal_title：Biotechnology and bioengineering

authors： Hradil G,Calo JM,Wunderlich TK Jr

更新日期：1993-02-05 00:00:00

abstract：:A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extr...

journal_title：Biotechnology and bioengineering

authors： Kempe H,Persson P,Axelsson A,Nilsson B,Zacchi G

更新日期：2006-03-05 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:A stirred bath technique was developed for determining effective diffusivities in cell matrices. The technique involves cell immobilization in a dilute gel which has negligible effect on solute diffusion. Agar and collagen were tested as immobilizing gels. Agar gel was shown to have minor interactions with the diffusi...

journal_title：Biotechnology and bioengineering

authors： Chresand TJ,Dale BE,Hanson SL,Gillies RJ

更新日期：1988-10-05 00:00:00

abstract：:Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cyt...

journal_title：Biotechnology and bioengineering

authors： Prudhomme WA,Duggar KH,Lauffenburger DA

更新日期：2004-11-05 00:00:00

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00