Abstract:
:A cyclic fed-batch bioprocess is designed and a significant improvement of rice alpha-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media for cell growth and enzyme production phases, and process control strategy enabling high cell-density culture and high alpha-amylase productivity. This process control can be achieved through maintaining a constant optimal specific cell growth rate at a predetermined value (i.e., 0.1 h-1), controlling medium feed rate commensurate with the cell growth rate, and maintaining a high cell-density culture (i.e., 60-70 g/L) for high productivity of cloned heterologous protein. The volumetric enzyme productivity (1, 960 units/L. h) achieved from the cyclic fed-batch process was about 3-fold higher than that of the fed-batch culture process (630 units/L. h).
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Chang CC,Ryu DD,Park CS,Kim JYdoi
10.1002/(sici)1097-0290(19980805)59:3<379::aid-bitsubject
Has Abstractpub_date
1998-08-05 00:00:00pages
379-85issue
3eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19980805)59:3<379::AID-BITjournal_volume
59pub_type
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