Enhancement of pyruvate production by osmotic-tolerant mutant of Torulopsis glabrata.

abstract：:The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Quo M

更新日期：1991-02-20 00:00:00

abstract：:Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces alpha-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177), which were...

journal_title：Biotechnology and bioengineering

authors： Bocking SP,Wiebe MG,Robson GD,Hansen K,Christiansen LH,Trinci AP

更新日期：1999-12-20 00:00:00

abstract：:The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...

journal_title：Biotechnology and bioengineering

authors： Park TH,Kim IH,Chang HN

更新日期：1985-08-01 00:00:00

abstract：:The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

journal_title：Biotechnology and bioengineering

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-03-20 00:00:00

abstract：:Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

journal_title：Biotechnology and bioengineering

authors： Nishikawa M

更新日期：1991-03-25 00:00:00

abstract：:Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with hi...

journal_title：Biotechnology and bioengineering

authors： Segura MM,Kamen A,Trudel P,Garnier A

更新日期：2005-05-20 00:00:00

abstract：:The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...

journal_title：Biotechnology and bioengineering

authors： Hannoun BJ,Stephanopoulos G

更新日期：1986-06-01 00:00:00

abstract：:Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

journal_title：Biotechnology and bioengineering

authors： Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

更新日期：2017-10-01 00:00:00

abstract：:A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical consideratio...

journal_title：Biotechnology and bioengineering

authors： Nath S

更新日期：1996-01-05 00:00:00

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

journal_title：Biotechnology and bioengineering

authors： Marek M,Valentová O,Kás J

更新日期：1984-10-01 00:00:00

abstract：:Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...

journal_title：Biotechnology and bioengineering

authors： Derksen JT,Boswinkel G,Cuperus FP

更新日期：1992-10-05 00:00:00

abstract：:Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

journal_title：Biotechnology and bioengineering

authors： Doran JB,Aldrich HC,Ingram LO

更新日期：1994-06-20 00:00:00

abstract：:10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

journal_title：Biotechnology and bioengineering

authors： Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

更新日期：1995-12-05 00:00:00

abstract：:Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

journal_title：Biotechnology and bioengineering

authors： Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

更新日期：2019-08-01 00:00:00

abstract：:This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

journal_title：Biotechnology and bioengineering

authors： Wang QQ,Zhu JY,Hunt CG,Zhan HY

更新日期：2012-08-01 00:00:00

abstract：:Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

journal_title：Biotechnology and bioengineering

authors： Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

更新日期：2020-05-01 00:00:00

abstract：:Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

journal_title：Biotechnology and bioengineering

authors： Miyasaka Y,Rha C,Sinskey AJ

更新日期：1980-10-01 00:00:00

abstract：:Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

journal_title：Biotechnology and bioengineering

authors： Chen V,Kim KJ,Fane AG

更新日期：1995-07-20 00:00:00

abstract：:Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...

journal_title：Biotechnology and bioengineering

authors： Peyton SR,Kalcioglu ZI,Cohen JC,Runkle AP,Van Vliet KJ,Lauffenburger DA,Griffith LG

更新日期：2011-05-01 00:00:00

abstract：:Mixed culture of microorganisms immobilized onto Celite diatomaceous earth particles were used to degrade 3,4-dichloroaniline (34DCA) in a three-phase draft tube fluidized bed bioreactor. Biodegradation was confirmed as the dominant removal mechanism by measurements of the concomitant chloride ion evolution. Degradati...

journal_title：Biotechnology and bioengineering

authors： Livingston AG

更新日期：1991-07-01 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

journal_title：Biotechnology and bioengineering

authors： Byk G,Soto J,Mattler C,Frederic M,Scherman D

更新日期：1998-04-01 00:00:00

abstract：:The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...

journal_title：Biotechnology and bioengineering

authors： Umaña P,Jean-Mairet J,Bailey JE

更新日期：1999-12-05 00:00:00

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

journal_title：Biotechnology and bioengineering

authors： Seo JH,Bailey JE

更新日期：1985-02-01 00:00:00

abstract：:Morphology has a crucial effect on productivity and the supply of substrate for cultures of filamentous fungi. However, cultivation parameters leading to the desired morphology are often chosen empirically as the mechanisms governing the processes involved are usually unknown. For coagulating microorganisms like Asper...

journal_title：Biotechnology and bioengineering

authors： Grimm LH,Kelly S,Hengstler J,Göbel A,Krull R,Hempel DC

更新日期：2004-07-20 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

journal_title：Biotechnology and bioengineering

authors： Evans LL,Burns MA

更新日期：1995-12-05 00:00:00