Enhancement of pyruvate production by osmotic-tolerant mutant of Torulopsis glabrata.


:Pyruvate production by Torulopsis glabrata was used as a model to study the mechanism of product inhibition and the strategy for enhancing pyruvate production. It was found that the concentration of cell growth and pyruvate deceased with the increase of NaCl and sorbitol concentrations. To enhance the osmotic stress resistance of the strain, an NaCl-tolerant mutant RS23 was screened and selected through a pH-controlled continuous culture with 70 g/L NaCl as the selective criterion. Compared with the parent strain, mutant RS23 could grow well on the medium containing 70 g/L NaCl or 0.6 mol/L sorbitol. Pyruvate concentration by the mutant strain RS23 reached 94.3 g/L at 82 h (yield on glucose 0.635 g/g) in a 7-l fermentor with 150 g/L glucose as carbon source. Pyruvate concentration and yield of mutant RS23 were 41.1% and 11.1% higher than those of the parent strain, respectively. The strategy for enhancing pyruvate production by increasing osmotic stress resistance may provide an alternative approach to enhance organic acids production with yeast.


Biotechnol Bioeng


Liu L,Xu Q,Li Y,Shi Z,Zhu Y,Du G,Chen J




Has Abstract


2007-07-01 00:00:00












  • A kinetic model for methanogenesis from whey permeate in a packed bed immobilized cell bioreactor.

    abstract::The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang ST,Quo M

    更新日期:1991-02-20 00:00:00

  • Effect of branch frequency in Aspergillus oryzae on protein secretion and culture viscosity.

    abstract::Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces alpha-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177), which were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bocking SP,Wiebe MG,Robson GD,Hansen K,Christiansen LH,Trinci AP

    更新日期:1999-12-20 00:00:00

  • Recycle hollow fiber enzyme reactor with flow swing.

    abstract::The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park TH,Kim IH,Chang HN

    更新日期:1985-08-01 00:00:00

  • In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium.

    abstract::The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Thompson DN,Hames BR,Reddy CA,Grethlein HE

    更新日期:1998-03-20 00:00:00

  • On the estimation of average shear rate in bubble columns.

    abstract::Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nishikawa M

    更新日期:1991-03-25 00:00:00

  • A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography.

    abstract::Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with hi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Segura MM,Kamen A,Trudel P,Garnier A

    更新日期:2005-05-20 00:00:00

  • Diffusion coefficients of glucose and ethanol in cell-free and cell-occupied calcium alginate membranes.

    abstract::The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hannoun BJ,Stephanopoulos G

    更新日期:1986-06-01 00:00:00

  • Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors.

    abstract::Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

    更新日期:2017-10-01 00:00:00

  • A rapid method for determining kinetic parameters of enzymes exhibiting nonlinear thermal inactivation behavior.

    abstract::A rapid method is developed to analyze the kinetics of thermal inactivation of enzymes that exhibit a nonlinear biphasic log(activity)-time relationship. Thermal destruction experiments on alcohol dehydrogenase from baker's yeast demonstrate the applicability of the method. The method is based on physical consideratio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nath S

    更新日期:1996-01-05 00:00:00

  • Potential application of monolith packed columns as bioreactors, control of biofilm formation.

    abstract::Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

    更新日期:2006-02-05 00:00:00

  • Invertase immobilization via its carbohydrate moiety.

    abstract::After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Marek M,Valentová O,Kás J

    更新日期:1984-10-01 00:00:00

  • Cellulase activity in commercial lipase preparations.

    abstract::Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Derksen JT,Boswinkel G,Cuperus FP

    更新日期:1992-10-05 00:00:00

  • Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway.

    abstract::Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Doran JB,Aldrich HC,Ingram LO

    更新日期:1994-06-20 00:00:00

  • Fermentation and isolation of C10-deacetylase for the production of 10-deacetylbaccatin III from baccatin III.

    abstract::10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

    更新日期:1995-12-05 00:00:00

  • Construction of second generation protease-deficient hosts of Bacillus subtilis for secretion of foreign proteins.

    abstract::Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

    更新日期:2019-08-01 00:00:00

  • Kinetics of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions.

    abstract::This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang QQ,Zhu JY,Hunt CG,Zhan HY

    更新日期:2012-08-01 00:00:00

  • Truly continuous low pH viral inactivation for biopharmaceutical process integration.

    abstract::Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

    更新日期:2020-05-01 00:00:00

  • Application of temperature-sensitive mutants for single-cell protein production.

    abstract::Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Miyasaka Y,Rha C,Sinskey AJ

    更新日期:1980-10-01 00:00:00

  • Effect of membrane morphology and operation on protein deposition in ultrafiltration membranes.

    abstract::Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chen V,Kim KJ,Fane AG

    更新日期:1995-07-20 00:00:00

  • Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness.

    abstract::Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Peyton SR,Kalcioglu ZI,Cohen JC,Runkle AP,Van Vliet KJ,Lauffenburger DA,Griffith LG

    更新日期:2011-05-01 00:00:00

  • Biodegradation of 3,4-dichloroaniline in a fluidized bed bioreactor and a steady-state biofilm Kinetic model.

    abstract::Mixed culture of microorganisms immobilized onto Celite diatomaceous earth particles were used to degrade 3,4-dichloroaniline (34DCA) in a three-phase draft tube fluidized bed bioreactor. Biodegradation was confirmed as the dominant removal mechanism by measurements of the concomitant chloride ion evolution. Degradati...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Livingston AG

    更新日期:1991-07-01 00:00:00

  • Dynamic pressure method for kla measurement in large-scale bioreactors.

    abstract::A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Linek V,Benes P,Vacek V

    更新日期:1989-05-01 00:00:00

  • Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

    abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Byk G,Soto J,Mattler C,Frederic M,Scherman D

    更新日期:1998-04-01 00:00:00

  • Tetracycline-regulated overexpression of glycosyltransferases in Chinese hamster ovary cells.

    abstract::The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. Here we use tetracyc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Umaña P,Jean-Mairet J,Bailey JE

    更新日期:1999-12-05 00:00:00

  • Effect of electrical charges and fields on injury and viability of airborne bacteria.

    abstract::In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

    更新日期:2002-07-20 00:00:00

  • A segregated model for plasmid content and product synthesis in unstable binary fission recombinant organisms.

    abstract::Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Seo JH,Bailey JE

    更新日期:1985-02-01 00:00:00

  • Kinetic studies on the aggregation of Aspergillus niger conidia.

    abstract::Morphology has a crucial effect on productivity and the supply of substrate for cultures of filamentous fungi. However, cultivation parameters leading to the desired morphology are often chosen empirically as the mechanisms governing the processes involved are usually unknown. For coagulating microorganisms like Asper...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Grimm LH,Kelly S,Hengstler J,Göbel A,Krull R,Hempel DC

    更新日期:2004-07-20 00:00:00

  • Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature.

    abstract::A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

    更新日期:2003-11-20 00:00:00

  • Application of cellulases from an alkalothermophilic Thermomonospora sp. in biopolishing of denims.

    abstract::Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Anish R,Rahman MS,Rao M

    更新日期:2007-01-01 00:00:00

  • Countercurrent gradient chromatography: A continuous focusing technique.

    abstract::The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Evans LL,Burns MA

    更新日期:1995-12-05 00:00:00