Magnetic resonance microscopy analysis of advective transport in a biofilm reactor.

abstract：:We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...

journal_title：Biotechnology and bioengineering

authors： Droz X,Harraghy N,Lançon E,Le Fourn V,Calabrese D,Colombet T,Liechti P,Rida A,Girod PA,Mermod N

更新日期：2017-08-01 00:00:00

abstract：:This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

journal_title：Biotechnology and bioengineering

authors： Rehmann L,Daugulis AJ

更新日期：2008-04-01 00:00:00

abstract：:A novel method has been developed for the separation of bioproducts from yeast cells. The method uses a combination of physical, chemical, and biological agents such as lytic enzymes, osmotic supports, and spheroplast stabilizers. Using this technique, products (proteins and enzymes) can be released from specific cell...

journal_title：Biotechnology and bioengineering

authors： Huang RB,Andrews BA,Asenjo JA

更新日期：1991-11-01 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench," is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term...

journal_title：Biotechnology and bioengineering

authors： Rachinskiy K,Schultze H,Boy M,Bornscheuer U,Büchs J

更新日期：2009-06-01 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...

journal_title：Biotechnology and bioengineering

authors： Shah NN,Park S,Taylor RT,Droege MW

更新日期：1992-09-01 00:00:00

abstract：:Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

journal_title：Biotechnology and bioengineering

authors： Nishikawa M

更新日期：1991-03-25 00:00:00

abstract：:Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

journal_title：Biotechnology and bioengineering

authors： Hjortso MA,Bailey JE

更新日期：1984-05-01 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

journal_title：Biotechnology and bioengineering

authors： Murray WD

更新日期：1987-06-01 00:00:00

abstract：:A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Kozaki Y,Kodama H,Shioya S

更新日期：1999-03-05 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:Extractive work-up of whole-cell biotransformations generally suffers from the formation of stable gels and slimes upon addition of the organic solvent to the cell suspension and the cell-free solution, respectively. This problem has been overcome by enzymatic lysis of emulsifying agents present in the medium through ...

journal_title：Biotechnology and bioengineering

authors： Jörg G,Leppchen K,Daussmann T,Bertau M

更新日期：2004-08-20 00:00:00

abstract：:Pre-filtration using ion exchange membrane adsorbers can improve parvovirus filter throughput of monoclonal antibodies (mAbs). The membranes work by binding trace foulants, and although some antibody product also binds, yields > or =99% are easily achieved by overloading. Results show that foulant adsorption is depend...

journal_title：Biotechnology and bioengineering

authors： Brown A,Bechtel C,Bill J,Liu H,Liu J,McDonald D,Pai S,Radhamohan A,Renslow R,Thayer B,Yohe S,Dowd C

更新日期：2010-07-01 00:00:00

abstract：:This study examined the hydrodynamic characteristics of a liquid-solid fluidized-bed bioreactor using elastic particles (PVA gel beads) of various diameters as carriers. The drag coefficient-Reynolds number, velocity-voidage, and expansion index-Reynolds number relationships observed during fluidization of PVA gel bea...

journal_title：Biotechnology and bioengineering

authors： Wu JY,Chen KC,Chen CT,Hwang SC

更新日期：2003-09-05 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...

journal_title：Biotechnology and bioengineering

authors： Meyer F,Gehmlich I,Guthke R,Górak A,Knorre WA

更新日期：1998-07-20 00:00:00

abstract：:The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state perfo...

journal_title：Biotechnology and bioengineering

authors： Park Y,Davis ME,Wallis DA

更新日期：1984-05-01 00:00:00

abstract：:The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitri...

journal_title：Biotechnology and bioengineering

authors： Chen X,Guo J,Xie GJ,Yuan Z,Ni BJ

更新日期：2016-05-01 00:00:00

abstract：:The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity-time trajectories for a microheterogeneous enzyme. Using an example, the model is ...

journal_title：Biotechnology and bioengineering

authors： Malhotra A,Sadana A

更新日期：1987-07-01 00:00:00

abstract：:A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio-)molecules and reduction or exchange of the salt buffer. Differing from the single-pass UF concepts known from the literature, DF operation does not require the application of sev...

journal_title：Biotechnology and bioengineering

authors： Tan R,Franzreb M

更新日期：2020-03-01 00:00:00

abstract：:RNA viruses are capable of undergoing extremely rapid evolution due to their high rates of reproduction, small genome size, and a high frequency of spontaneous mutagenesis. Here we demonstrate that a virus-like, evolutionary state can be created by propagating a phagemid population in a hypermutator strain of Escheric...

journal_title：Biotechnology and bioengineering

authors： Long-McGie J,Liu AD,Schellenberger V

更新日期：2000-04-05 00:00:00

abstract：:A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in...

journal_title：Biotechnology and bioengineering

authors： Dubey H,Das SK,Panda T

更新日期：2006-11-05 00:00:00

abstract：:Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

journal_title：Biotechnology and bioengineering

authors： Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

更新日期：2010-06-01 00:00:00

abstract：:Polypeptides containing between 4 and 32 repeats of a resilin-inspired sequence AQTPSSYGAP, derived from the mosquito Anopheles gambiae, have been used as tags on recombinant fusion proteins. These repeating polypeptides were inspired by the repeating structures that are found in resilins and sequence-related proteins...

journal_title：Biotechnology and bioengineering

authors： Lyons RE,Elvin CM,Taylor K,Lekieffre N,Ramshaw JA

更新日期：2012-12-01 00:00:00

abstract：:Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...

journal_title：Biotechnology and bioengineering

authors： Mehta KK,Paskaleva EE,Wu X,Grover N,Mundra RV,Chen K,Zhang Y,Yang Z,Feng H,Dordick JS,Kane RS

更新日期：2016-12-01 00:00:00

abstract：:PER.C6 cells were cultivated for propagation of a replication-defective adenovirus vector in serum-free suspension bioreactors. Cellular metabolism during cell growth and adenovirus propagation was fully characterized using on-line and off-line methods. The energy metabolism was found to accelerate transiently after a...

journal_title：Biotechnology and bioengineering

authors： Maranga L,Auniņs JG,Zhou W

更新日期：2005-06-05 00:00:00

abstract：:A new biomass productivity model was parameterized for Chlorella vulgaris using short-term (<30 min) oxygen productivities from algal microcosms exposed to 6 light intensities (20-420 W/m(2)) and 6 temperatures (5-42 °C). The model was then validated against experimental biomass productivities recorded in bench-scale ...

journal_title：Biotechnology and bioengineering

authors： Béchet Q,Chambonnière P,Shilton A,Guizard G,Guieysse B

更新日期：2015-05-01 00:00:00

abstract：:Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

journal_title：Biotechnology and bioengineering

authors： Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

更新日期：2008-07-01 00:00:00