H(2) photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor.

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

journal_title：Biotechnology and bioengineering

authors： Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

更新日期：2000-12-20 00:00:00

abstract：:A conceptual model describing the relative roles of sorption and precipitation processes for metals in solid-solution suspensions is presented. The model performance is demonstrated using experimental data on sorption and precipitation of metals in samples of activated sludge mixed liquor. Based on the experimental re...

journal_title：Biotechnology and bioengineering

authors： Kodukula PS,Patterson JW,Surampalli RY

更新日期：1994-04-15 00:00:00

abstract：:Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1994-05-01 00:00:00

abstract：:The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

journal_title：Biotechnology and bioengineering

authors： van de Walle M,Shiloach J

更新日期：1998-01-05 00:00:00

abstract：:The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of Bacillus subtilis grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) was controlled, the maximum specific g...

journal_title：Biotechnology and bioengineering

authors： Suphantharika M,Ison AP,Lilly MD,Buckland BC

更新日期：1994-10-01 00:00:00

abstract：:A starvation-based dissolved oxygen (DO) transient controller was developed to supply growth-limiting substrate to high cell density fed-batch cultures of recombinant Escherichia coli. The algorithm adjusted a preexisting feed rate in proportion to the culture's oxygen demand, which was estimated from transients in th...

journal_title：Biotechnology and bioengineering

authors： Whiffin VS,Cooney MJ,Cord-Ruwisch R

更新日期：2004-02-20 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:Fatty aldehydes and alcohols are valuable precursors used in the industrial manufacturing of a myriad of specialty products. Herein, we demonstrate the de novo production of odd chain-length fatty aldehydes and fatty alcohols in Saccharomyces cerevisiae by expressing a novel biosynthetic pathway involving cytosolic th...

journal_title：Biotechnology and bioengineering

authors： Jin Z,Wong A,Foo JL,Ng J,Cao YX,Chang MW,Yuan YJ

更新日期：2016-04-01 00:00:00

abstract：:We propose an in vitro selection strategy to identify bacteriophage variants that recognize metal ions in solution. In 6 M urea, phage T7 loses 99.9% of its activity in less than 5 min. Inactivation is accelerated by gold, but slowed by zinc and magnesium. Selection of phage over five generations in the presence of go...

journal_title：Biotechnology and bioengineering

authors： Gupta K,Yin J

更新日期：1995-03-05 00:00:00

abstract：:A priori knowledge of the dissolved oxygen (O2) concentration profile within a hepatic hollow fiber (HF) bioreactor is important in developing an effective bioartificial liver assist device (BLAD). O2 provision is limiting within HF bioreactors and we hypothesize that supplementing a hepatic HF bioreactor's circulatin...

journal_title：Biotechnology and bioengineering

authors： Sullivan JP,Gordon JE,Palmer AF

更新日期：2006-02-05 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:Microbial secondary metabolites are one of the sources of therapeutic molecules in the pharmaceutical industry. Product quality and high yields of secondary metabolites are the main goals for the commercial success of a fermentation process. Our novel approach was based on the decision-tree algorithm to determine the ...

journal_title：Biotechnology and bioengineering

authors： Bradamante S,Barenghi L,Beretta G,Bonfa' M,Rollini M,Manzoni M

更新日期：2002-12-05 00:00:00

abstract：:Microbial kinetics and energetics are discussed in connection with the formulation of unstructured growth models. The development of microbial energetics and the use of macroscopic methods in the study of microbial growth are briefly evaluated. The general approach to the modelling of microbial growth has been critica...

journal_title：Biotechnology and bioengineering

authors： Esener AA,Roels JA,Kossen NW

更新日期：1983-12-01 00:00:00

abstract：:The direct microbial conversion (DMC) process for the production of ethanol from lignocellulosic biomass is limited by low volumetric ethanol production rates due to the low cell densities of Clostridium thermosaccharolyticum which is a key organism for ethanol production in this process. Hence, this study focuses on ...

journal_title：Biotechnology and bioengineering

authors： Mistry FR,Cooney CL

更新日期：1989-12-05 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Efficient xylose utilization will facilitate microbial conversion of lignocellulosic sugar mixtures into valuable products. In Escherichia coli, xylose catabolism is controlled by carbon catabolite repression (CCR). However, in E. coli such as the succinate-producing strain KJ122 with disrupted CCR, xylose utilization...

journal_title：Biotechnology and bioengineering

authors： Kurgan G,Sievert C,Flores A,Schneider A,Billings T,Panyon L,Morris C,Taylor E,Kurgan L,Cartwright R,Wang X

更新日期：2019-08-01 00:00:00

abstract：:Chemotaxis has been shown to be beneficial for the migration of soil-inhabiting bacteria towards industrial chemical pollutants, which they degrade. Many studies have demonstrated the importance of this microbial property under various circumstances; however, few quantitative analyses have been undertaken to measure t...

journal_title：Biotechnology and bioengineering

authors： Wang X,Atencia J,Ford RM

更新日期：2015-05-01 00:00:00

abstract：:Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...

journal_title：Biotechnology and bioengineering

authors： Sivakumar G,Liu C,Towler MJ,Weathers PJ

更新日期：2010-12-01 00:00:00

abstract：:Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...

journal_title：Biotechnology and bioengineering

authors： Racine J,Cheradame H,Chu YS,Thiery JP,Rodriguez I

更新日期：2011-04-01 00:00:00

abstract：:Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...

journal_title：Biotechnology and bioengineering

authors： Adachi M,Yamazaki M,Harada M,Shioi A,Katch S

更新日期：1997-02-20 00:00:00

abstract：:Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

journal_title：Biotechnology and bioengineering

authors： Nishikawa M

更新日期：1991-03-25 00:00:00

abstract：:Predicting and improving oxygen transport within bioartificial liver (BAL) devices continues to be an important engineering challenge since oxygen is one of the critical nutrients necessary for maintaining hepatocyte viability and function. Such a computational model would not only help predict outcomes but it would a...

journal_title：Biotechnology and bioengineering

authors： McClelland RE,MacDonald JM,Coger RN

更新日期：2003-04-05 00:00:00

abstract：:Biofilms commonly develop in flowing aqueous environments, where the flow causes the biofilm to deform. Because biofilm deformation affects the flow regime, and because biofilms behave as complex heterogeneous viscoelastic materials, few models are able to predict biofilm deformation. In this study, a phase-field (PF)...

journal_title：Biotechnology and bioengineering

authors： Li M,Matouš K,Nerenberg R

更新日期：2020-07-13 00:00:00

abstract：:The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

journal_title：Biotechnology and bioengineering

authors： Kheirolomoom A,Katoh S,Sada E,Yoshida K

更新日期：1991-04-15 00:00:00

abstract：:Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical ...

journal_title：Biotechnology and bioengineering

authors： Tashima AK,Ottens M,Van der Wielen LA,Cintra DE,Pauli JR,Filho Pde A,Miranda EA

更新日期：2009-08-01 00:00:00

abstract：:Cytochrome c-oxidase, used as a model membrane-bound enzyme, was embedded in phospholipid bilayer membranes, attached to nanometer-sized Fe(3)O(4) colloids (so-called magnetoliposomes). In comparison with the lipid-depleted free enzyme, both the activity and the enzymatic stability of the complexes, stored at 4 degree...

journal_title：Biotechnology and bioengineering

authors： De Cuyper M,De Meulenaer B,Van der Meeren P,Vanderdeelen J

更新日期：1996-03-20 00:00:00

abstract：:Many biochemical processes consist of a sequence of operations for which optimal operating conditions (setpoints) have to be determined. If such optimization is performed for each operation separately with respect to objectives defined for each operation individually, overall process performance is likely to be subopt...

journal_title：Biotechnology and bioengineering

authors： Groep ME,Gregory ME,Kershenbaum LS,Bogle ID

更新日期：2000-02-05 00:00:00

abstract：:The engineering of production cell lines to express anti-apoptotic genes has been pursued in recent years due to potential process benefits, including enhanced cell survival, increased protein expression, and improved product quality. In this study, a baby hamster kidney cell line secreting recombinant factor VIII (BH...

journal_title：Biotechnology and bioengineering

authors： Nivitchanyong T,Martinez A,Ishaque A,Murphy JE,Konstantinov K,Betenbaugh MJ,Thrift J

更新日期：2007-11-01 00:00:00

abstract：:Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...

journal_title：Biotechnology and bioengineering

authors： Hafiz EOA,Bulutoglu B,Mansy SS,Chen Y,Abu-Taleb H,Soliman SAM,El-Hindawi AAF,Yarmush ML,Uygun BE

更新日期：2021-01-01 00:00:00