Abstract:
:In order to carry out an effective enzyme reaction, the preparation of soluble-insoluble immobilized enzyme was investigated. Proteases were selected as model enzymes, and their immobilization was carried out by using an enteric coating polymer as a carrier. Among the polymers tested, methacrylic acid-methylacrylate-methylmethacrylate copolymer (MPM-06) gave the most active soluble-insoluble immobilized papain. This immobilized papain showed insoluble from below pH 4.8 and soluble form above pH 5.8; it was also soluble in water-miscible organic solvent. It was reusable and more stable with heat and water-miscible organic solvents than native proteases. Furthermore, various proteases could be immobilized by using MPM-06 with high activity. Chymotrypsin immobilized by this method catalyzed the effective peptide synthesis in a heterogeneous reaction system containing water-miscible organic solvent.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Fujimura M,Mori T,Tosa Tdoi
10.1002/bit.260290612subject
Has Abstractpub_date
1987-04-01 00:00:00pages
747-52issue
6eissn
0006-3592issn
1097-0290journal_volume
29pub_type
杂志文章abstract::Current techniques for characterizing biofilm physiology lack the signal filtering capability required for quantifying signals associated with real time biologically active transport. Though a great deal was learned from previous investigations, no results have been reported on the characterization of in vivo, real ti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22128
更新日期:2009-02-15 00:00:00
abstract::The inhibition of the maximum specific growth and fermentation rate of Zymomonas mobilis by ethanol was studied in turbidostat cultures at constant and stepwise changed ethanol concentrations. Up to 50 g/L ethanol, the inhibition kinetics can be approximated by a linear relationship between the specific growth rate an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280412
更新日期:1986-04-01 00:00:00
abstract::This article deals with the modeling of the oxygen transfer in an industrial airlift fermentor used for lactic yeast production on whey substrates. The purpose of this study was to improve the understanding of the interactions among the various parameters that govern the oxygen transfer phenomena in this type of ferme...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390907
更新日期:1992-04-15 00:00:00
abstract::James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...
journal_title:Biotechnology and bioengineering
pub_type: 传,历史文章,杂志文章
doi:10.1002/bit.10404
更新日期:2002-09-05 00:00:00
abstract::Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21523
更新日期:2008-01-01 00:00:00
abstract::Production of conidia of the biocontrol fungus Coniothyrium minitans by solid-state cultivation in a packed-bed reactor on an industrial scale is feasible. Spore yield and oxygen consumption rate of C. minitans during cultivation on oats and three inert solids (hemp, perlite, and bagasse) saturated with a liquid mediu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19991120)65:4<447::aid-bit
更新日期:1999-11-20 00:00:00
abstract::Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24583
更新日期:2012-12-01 00:00:00
abstract::One of the major bottlenecks in the bioconversion of lignocelluosic feedstocks to liquid ethanol is the recalcitrance of residue following pretreatment, specifically softwood derived residues. Peroxide delignification has previously been shown to effectively aid in the removal of condensed lignaceous moieties from sub...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22768
更新日期:2010-08-15 00:00:00
abstract::The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27113
更新日期:2019-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980905)59:5<544::aid-bit
更新日期:1998-09-05 00:00:00
abstract::Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assem...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260181204
更新日期:1976-12-01 00:00:00
abstract::The application of enantioseparation methods alone can only yield up to 50% of the desired chiral product. Thus enantioseparation becomes more attractive when accompanied by the racemization of the counter-enantiomer. Here we present first results of dynamic kinetic resolution of L-asparagine (L-Asn) via preferential ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22498
更新日期:2009-12-15 00:00:00
abstract::Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23231
更新日期:2011-11-01 00:00:00
abstract::Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22149
更新日期:2009-03-01 00:00:00
abstract::The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260281110
更新日期:1986-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360811
更新日期:1990-10-20 00:00:00
abstract::Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390405
更新日期:1992-02-20 00:00:00
abstract::A computer-controlled headspace gas chromatograph was used to monitor the progress of ethanol production from both aerobic batch and anaerobic continuous fermentations. Using an automatic, electropneumatic sampling system, aliquots of fermentation headspace gas were injected directly onto the column for quantitative e...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251103
更新日期:1983-11-01 00:00:00
abstract::Five microbial inocula were evaluated in batch tests for the ability to remediate mine drainage (MD). Dairy manure (DM), anaerobic digester sludge, substrate from the Luttrell (LUTR) and Peerless Jenny King (PJK) sulfate-reducing permeable reactive zones (SR-PRZs) and material from an MD-treatment column that had been...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21930
更新日期:2008-11-01 00:00:00
abstract::The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380212
更新日期:1991-06-20 00:00:00
abstract::The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990720)64:2<240::aid-bit
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abstract::In our previous study [Hong Y, Brown DG (2009) Appl Environ Microbiol 75(8):2346-2353], the adenosine triphosphate (ATP) level of adhered bacteria was observed to be 2-5 times higher than that of planktonic bacteria. Consequently, the proton motive force (Delta p) of adhered bacteria was approximately 15% greater than...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22606
更新日期:2010-04-01 00:00:00
abstract::The regeneration of functional, clinically viable, tissues from acellular ex vivo tissues has been problematic largely due to poor nutrient transport conditions that limit cell migration and integration. Compounding these issues are subcellular pore sizes that necessarily requires extracellular matrix (ECM) remodeling...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24934
更新日期:2013-07-01 00:00:00
abstract::Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, while the combined eff...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-01-05 00:00:00
abstract::A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250209
更新日期:1983-02-01 00:00:00
abstract::After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24750
更新日期:2013-03-01 00:00:00
abstract::Lentiviral vectors (LVs) enveloped with an engineered Sindbis virus glycoprotein can specifically bind to dendritic cells (DCs) through the surface receptor DC-SIGN and induce antigen expression, thus providing an efficient method for delivering DC-directed vaccines. In this study, we constructed a stable producer lin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24413
更新日期:2012-06-01 00:00:00
abstract::A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20069
更新日期:2004-06-20 00:00:00
abstract::A microarray targeting four sequenced strains in the Dehalococcoides (Dhc) genus was used to analyze gene expression in a robust long-term trichloroethene (TCE)-degrading microbial community (designated ANAS) during feeding cycles that involve conditions of periodic substrate supply. The Dhc transcriptome was examined...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24819
更新日期:2013-05-01 00:00:00
abstract::Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26923
更新日期:2019-05-01 00:00:00