Growth of Leuconostoc mesenteroides NRRL-B523 in an alkaline medium: suboptimal pH growth inhibition of a lactic acid bacterium.

abstract：:Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...

journal_title：Biotechnology and bioengineering

authors： Barminko JA,Nativ NI,Schloss R,Yarmush ML

更新日期：2014-11-01 00:00:00

abstract：:Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...

journal_title：Biotechnology and bioengineering

authors： Freed LE,Marquis JC,Langer R,Vunjak-Novakovic G,Emmanual J

更新日期：1994-03-25 00:00:00

abstract：:The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state perfo...

journal_title：Biotechnology and bioengineering

authors： Park Y,Davis ME,Wallis DA

更新日期：1984-05-01 00:00:00

abstract：:In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic ...

journal_title：Biotechnology and bioengineering

authors： Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

更新日期：2020-04-01 00:00:00

abstract：:Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the devel...

journal_title：Biotechnology and bioengineering

authors： DeMartini JD,Studer MH,Wyman CE

更新日期：2011-02-01 00:00:00

abstract：:Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell sorting (FACS). For comparison, cell pools and cell lines were al...

journal_title：Biotechnology and bioengineering

authors： Oberbek A,Matasci M,Hacker DL,Wurm FM

更新日期：2011-03-01 00:00:00

abstract：:Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment...

journal_title：Biotechnology and bioengineering

authors： Maes F,Van Ransbeeck P,Van Oosterwyck H,Verdonck P

更新日期：2009-06-15 00:00:00

abstract：:The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resulting from lyophilizati...

journal_title：Biotechnology and bioengineering

authors： Ru MT,Dordick JS,Reimer JA,Clark DS

更新日期：1999-04-20 00:00:00

abstract：:We present a mathematical model which includes TNF-alpha initiated survival and apoptotic cascades, as well as nuclear transcription of IkappaB. These pathways play a crucial role in deciding cell fate in response to inflammation and infection. Our model incorporates known specific protein-protein interactions as iden...

journal_title：Biotechnology and bioengineering

authors： Rangamani P,Sirovich L

更新日期：2007-08-01 00:00:00

abstract：:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

journal_title：Biotechnology and bioengineering

authors： Yu J,Wang J

更新日期：2001-06-20 00:00:00

abstract：:Biofouling is the undesirable adhesion and development of microorganisms and macroorganisms in a water environment. An electrochemical antifouling system based on management of primary adhesion of microorganisms was developed employing titanium electrode for antifouling of seawater cooling pipes and marine infrastruct...

journal_title：Biotechnology and bioengineering

authors： Wake H,Takahashi H,Takimoto T,Takayanagi H,Ozawa K,Kadoi H,Okochi M,Matsunaga T

更新日期：2006-10-20 00:00:00

abstract：:Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating electrochemical eff...

journal_title：Biotechnology and bioengineering

authors： Fan W,Bakir U,Glatz CE

更新日期：1998-08-20 00:00:00

abstract：:Escherichia coli and Desulfovibrio desulfuricans reduce Tc(VII) (TcO(4)(-)) with formate or hydrogen as electron donors. The reaction is catalyzed by the hydrogenase component of the formate hydrogenlyase complex (FHL) of E. coli and is associated with a periplasmic hydrogenase activity in D. desulfuricans. Tc(VII) re...

journal_title：Biotechnology and bioengineering

authors： Lloyd JR,Thomas GH,Finlay JA,Cole JA,Macaskie LE

更新日期：1999-01-01 00:00:00

abstract：:A novel process has been developed which uses reversed micelles to isolate denatured protein molecules from each other and allows them to refold individually. These reversed micelles are aqueous phase droplets stabilized by the surfactant AOT and suspended in isooctane. By adjusting conditions such that only one prote...

journal_title：Biotechnology and bioengineering

authors： Hagen AJ,Hatton TA,Wang DI

更新日期：1990-04-25 00:00:00

abstract：:Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

journal_title：Biotechnology and bioengineering

authors： Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

更新日期：1999-05-20 00:00:00

abstract：:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

journal_title：Biotechnology and bioengineering

authors： Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

更新日期：2020-06-01 00:00:00

abstract：:Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a s...

journal_title：Biotechnology and bioengineering

authors： Sucosky P,Osorio DF,Brown JB,Neitzel GP

更新日期：2004-01-05 00:00:00

abstract：:Escherichia coli has been the host organism most frequently investigated for efficient recombinant protein production. However, the production of a foreign protein in recombinant E. coli often leads to growth deterioration and elevated secretion of acetic acid. Such observed phenomena have been widely linked with cell...

journal_title：Biotechnology and bioengineering

authors： Zeng H,Yang A

更新日期：2019-06-01 00:00:00

abstract：:Innovation in monoclonal antibody (mAb) production continues to be driven by cell engineering strategies to increase yield and improve product quality. In a previous study, to investigate the effectiveness of transporter overexpression strategies, we prepared a taurine transporter-overexpressing host cell line (DXB11/...

journal_title：Biotechnology and bioengineering

authors： Tabuchi H,Sugiyama T

更新日期：2013-08-01 00:00:00

abstract：:Two general models for batch simultaneous enzymatic and microbial reaction (SEMR) processes are presented, the second derived from and simpler than the first and accounting for enzyme denaturation. Using the second model and parameter values from the literature, simulation was used to examine a range of enzyme additio...

journal_title：Biotechnology and bioengineering

authors： Asenjo JA,Sun WH,Spencer JL

更新日期：1991-05-01 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

journal_title：Biotechnology and bioengineering

authors： Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

更新日期：2000-10-20 00:00:00

abstract：:The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Quo M

更新日期：1991-02-20 00:00:00

abstract：:Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...

journal_title：Biotechnology and bioengineering

authors： Kim B,Binkley R,Kim HU,Lee SY

更新日期：2018-10-01 00:00:00

abstract：:A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assu...

journal_title：Biotechnology and bioengineering

authors： Zangirolami TC,Johansen CL,Nielsen J,Jørgensen SB

更新日期：1997-12-20 00:00:00

abstract：:We present an evaluation of the qualitative and quantitative effects that high concentrations of benzene and toluene have on the growth rate of several pure cultures that use these compounds as their sole carbon and energy source. The cultures employed were five widely studied environmental isolates: Pseudomonas putid...

journal_title：Biotechnology and bioengineering

authors： Alagappan G,Cowan R

更新日期：2003-09-30 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...

journal_title：Biotechnology and bioengineering

authors： Bhargava S,Wenger KS,Marten MR

更新日期：2003-04-05 00:00:00

abstract：:Pulses of several nonreacting solutes were fed to a column containing immobilized enzyme catalyst beads. The response curves were recorded, and their first and second moments were determined by numerical integration. From the moment analysis, the void volume, internal porosity, and tortuosity of the support were calcu...

journal_title：Biotechnology and bioengineering

authors： Sirotti DA,Emery A

更新日期：1983-07-01 00:00:00

abstract：:A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochromec, and trytophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solut...

journal_title：Biotechnology and bioengineering

authors： Gupta RB,Han CJ,Johnston KP

更新日期：1994-09-20 00:00:00