Simulation of penicillin production in fed-batch cultivations using a morphologically structured model.

Abstract:

:A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assumed to take place in the subapical compartment and in the growing region of the hyphal compartment. Furthermore, it is inhibited by glucose. Model parameters are estimated using an evolutionary algorithm and fitting the model to a standard fed-batch cultivation. The model is validated on experimental data from three different fed-batch cultivations, including two repeated fed-batch cultivations. The model predictions show good agreement with the measurements of biomass and pencillin concentrations for all fed-batch cultivations. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 593-604, 1997.

journal_name

Biotechnol Bioeng

authors

Zangirolami TC,Johansen CL,Nielsen J,Jørgensen SB

doi

10.1002/(SICI)1097-0290(19971220)56:6<593::AID-BIT

subject

Has Abstract

pub_date

1997-12-20 00:00:00

pages

593-604

issue

6

eissn

0006-3592

issn

1097-0290

journal_volume

56

pub_type

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