Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme.

abstract：:Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems. In this study, an affinity system for plasmid DNA (pDNA) purification has been developed utilizing the interaction between the lac operon (lacO) sequence c...

journal_title：Biotechnology and bioengineering

authors： Forde GM,Ghose S,Slater NK,Hine AV,Darby RA,Hitchcock AG

更新日期：2006-09-05 00:00:00

abstract：:The kinetic properties of collagen-bound firefly luciferase have been investigated. Under definite hydrodynamic conditions with low agitation in the reaction medium, the observed behavior is modified compared to the enzyme free in solution: reducing the stirring rate decreases the observed enzymatic activity. But diff...

journal_title：Biotechnology and bioengineering

authors： Blum LJ,Coulet PR

更新日期：1986-08-01 00:00:00

abstract：:13C metabolic flux analysis (MFA) is based on carbon-labeling experiments where a specifically (13)C labeled substrate is fed. The labeled carbon atoms distribute over the metabolic network and the label enrichment of certain metabolic pools is measured by using different methods. Recently, MS methods have been dramat...

journal_title：Biotechnology and bioengineering

authors： Wahl SA,Dauner M,Wiechert W

更新日期：2004-02-05 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:Nucleotide sugar-dependent ("Leloir") glycosyltransferases (GTs), represent a new paradigm for the application of biocatalytic glycosylations to the production of fine chemicals. However, it remains to be shown that GT processes meet the high efficiency targets of industrial biotransformations. We demonstrate in this ...

journal_title：Biotechnology and bioengineering

authors： Schmölzer K,Lemmerer M,Gutmann A,Nidetzky B

更新日期：2017-04-01 00:00:00

abstract：:Many bioprocesses depend on the effective formation of a biofilm on a solid support. In the present study, three different surface treatments (sandblasting, pure-O(2) plasma, and He-O(2) plasma treatments) were conducted on polypropylene (PP) Pall rings used as a support in biotrickling filters for air pollution contr...

journal_title：Biotechnology and bioengineering

authors： Prado OJ,Popat SC,Chen G,Walker SL,Lafuente J,Gabriel D,Deshusses MA

更新日期：2009-08-15 00:00:00

abstract：:A continuous viral inactivation (CVI) chamber has been designed to operate with acceptable residence time distribution (RTD) characteristics. However, altering the CVI's geometry and operation to accommodate the scale was not obvious. In this work, we elucidate the influence of Dean vortices and leverage the transitio...

journal_title：Biotechnology and bioengineering

authors： Brown MR,Orozco R,Coffman J

更新日期：2020-03-01 00:00:00

abstract：:Two strains of Pseudomonas able to grow on phenol or p-nitrophenol (PNP) were isolated from sewage. Pseudomonas sp. PN101 mineralized and formed nitrite from PNP but did not mineralize phenol, and Pseudomonas sp. PH111 mineralized phenol but not PNP. Phenol increased the lag period before Pseudomonas sp. PN101 grew on...

journal_title：Biotechnology and bioengineering

authors： Murakami Y,Alexander M

更新日期：1989-02-20 00:00:00

abstract：:Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...

journal_title：Biotechnology and bioengineering

authors： Schlatmann JE,Moreno PR,Vinke JL,Ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1994-08-05 00:00:00

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glyc...

journal_title：Biotechnology and bioengineering

authors： Gawlitzek M,Valley U,Wagner R

更新日期：1998-03-05 00:00:00

abstract：:In analyzing microbial cellulose utilization, it would be useful to independently measure the mass concentration of cells and cellulase enzymes. Such measurements would allow investigation of the allocation of cellular resources between synthesis of cells and cellulase, in vivo cell- and cellulase-specific cellulose h...

journal_title：Biotechnology and bioengineering

authors： Lynd LR,Zhang Y

更新日期：2002-02-15 00:00:00

abstract：:The S-System formalism provides a popular, versatile and mathematically tractable representation of metabolic pathways. At steady-state, after a logarithmic transformation, the S-System representation reduces into a system of linear equations. Thus, the maximization of a particular metabolite concentration or a flux s...

journal_title：Biotechnology and bioengineering

authors： Petkov SB,Maranas CD

更新日期：1997-10-20 00:00:00

abstract：:Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

journal_title：Biotechnology and bioengineering

authors： Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

更新日期：2020-01-01 00:00:00

abstract：:Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

journal_title：Biotechnology and bioengineering

authors： Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

更新日期：2007-04-01 00:00:00

abstract：:Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...

journal_title：Biotechnology and bioengineering

authors： Ghosh P,Lin M,Vogel JH,Choy D,Haynes C,von Lieres E

更新日期：2014-08-01 00:00:00

abstract：:Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...

journal_title：Biotechnology and bioengineering

authors： Freed LE,Marquis JC,Langer R,Vunjak-Novakovic G,Emmanual J

更新日期：1994-03-25 00:00:00

abstract：:A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-ba...

journal_title：Biotechnology and bioengineering

authors： Tang YJ,Sapra R,Joyner D,Hazen TC,Myers S,Reichmuth D,Blanch H,Keasling JD

更新日期：2009-04-01 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...

journal_title：Biotechnology and bioengineering

authors： Goudar CT

更新日期：2011-10-01 00:00:00

abstract：:Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase G(1). Although this hypothesis ...

journal_title：Biotechnology and bioengineering

authors： Cazzador L,Mariani L

更新日期：1993-12-01 00:00:00

abstract：:Biomanufacturing exhibits inherent variability that can lead to variation in performance attributes and batch failure. To help ensure process consistency and product quality the development of predictive models and integrated control strategies is a promising approach. In this study, a feedback controller was develope...

journal_title：Biotechnology and bioengineering

authors： Schmitt J,Downey B,Beller J,Russell B,Quach A,Lyon D,Curran M,Mulukutla BC,Chu C

更新日期：2019-09-01 00:00:00

abstract：:The biological molecule responsible for the suppression of pyritic sulfur in fine coal simulated froth flotation treated with bacteria was identified. Protein was found to be the most effective agent in pyrite suppression of the three cell components (protein, lipid, and carbohydrate) assayed. Coal recovery and ash re...

journal_title：Biotechnology and bioengineering

authors： Stainthorpe AC

更新日期：1989-02-05 00:00:00

abstract：:A poly(acrylic acid)-polyethylene graft copolymer was prepared and used initially to couple to acid phosphatase, using soluble carbodiimides. Yields which were quite good were obtained with CMC but not with EDAC. The copolymers was used to couple trypsin using EEDQ. Several organic solvents were investigated for the p...

journal_title：Biotechnology and bioengineering

authors： Beddows CG,Gil MH,Guthrie JT

更新日期：1982-06-01 00:00:00

abstract：:A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...

journal_title：Biotechnology and bioengineering

authors： Agena SM,Pusey ML,Bogle ID

更新日期：1999-07-20 00:00:00

abstract：:Cytidine-5'-diphosphocholine (CDP-choline) is a widely used neuroprotective drug for multiple indications. In industry, CDP-choline is synthesized by a two-step cell culture/permeabilized cell biotransformation method because substrates often do not enter cells in an efficient manner. This study develops a novel one-s...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Liu Q,Liu H,Zhou X,Zhang Y,Cai M

更新日期：2020-05-01 00:00:00

abstract：:CHO cells express glycoproteins containing both the N-acetylneuraminic acid (Neu5Ac) and minor amounts of the N-glycolylneuraminic acid (Neu5Gc) forms of sialic acid. As Neu5Gc is not expressed in humans and can be recognized as a foreign epitope, there is the potential for immunogenicity issues for glycoprotein thera...

journal_title：Biotechnology and bioengineering

authors： Borys MC,Dalal NG,Abu-Absi NR,Khattak SF,Jing Y,Xing Z,Li ZJ

更新日期：2010-04-15 00:00:00

abstract：:A mixed culture of sulfate-reducing bacteria containing the species Desulfovibrio desulfuricans was used to study sulfate-reduction stoichiometry and kinetics using ethanol as the carbon source. Growth yield was lower, and kinetics were slower, for ethanol compared to lactate. Ethanol was converted into acetate and no...

journal_title：Biotechnology and bioengineering

authors： Nagpal S,Chuichulcherm S,Livingston A,Peeva L

更新日期：2000-12-05 00:00:00

abstract：:Anaerobic methanethiol (MT) degradation by mesophilic (30 degrees C) alkaliphilic (pH 10) communities was studied in a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor inoculated with a mixture of sediments from the Wadden Sea (The Netherlands), Soap Lake (Central Washington), and Russian soda lakes. MT degradatio...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,Bonilla-Salinas M,de Bok FA,Bruning H,Lens PN,Stams AJ,Janssen AJ

更新日期：2008-11-01 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00