Protein interaction affinity determination by quantitative FRET technology.

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:Both the forward and backward reactions of xylose isomerase (Sweetzyme Q) with xylose and glucose as substrates have been studied in terms of kinetics and thermodynamics. The relationship between the two reactions can thus be determined. Much attention has been given to the reaction with xylose as substrate. The optim...

journal_title：Biotechnology and bioengineering

authors： Olivier SP,du Toit PJ

更新日期：1986-05-01 00:00:00

abstract：:Pretreatment plays an important role in the efficient enzymatic hydrolysis of biomass into fermentable sugars for biofuels. A highly effective pretreatment method is reported for corn stover which combines mild alkali-extraction followed by ionic liquid (IL) dissolution of the polysaccharides and regeneration (recover...

journal_title：Biotechnology and bioengineering

authors： Geng X,Henderson WA

更新日期：2012-01-01 00:00:00

abstract：:We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

更新日期：2008-10-15 00:00:00

abstract：:A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...

journal_title：Biotechnology and bioengineering

authors： Tiller JC,Lee SB,Lewis K,Klibanov AM

更新日期：2002-08-20 00:00:00

abstract：:Equilibrium isotherms for adsorption of bovine serum albumin (BSA) on a new adsorbent, a strongly basic crosslinked chitosan (Chitopearl 2503), which is hard and is not compressed by pressure in a column, have been presented and compared with diethylaminoethyl (DEAE) Sepharose Fast Flow (hard gel). In Chitopearl 2503,...

journal_title：Biotechnology and bioengineering

authors： Yoshida H,Nishihara H,Kataoka T

更新日期：1994-05-01 00:00:00

abstract：:We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...

journal_title：Biotechnology and bioengineering

authors： Goudar CT

更新日期：2011-10-01 00:00:00

abstract：:Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

journal_title：Biotechnology and bioengineering

authors： Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

更新日期：1999-05-20 00:00:00

abstract：:The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iro...

journal_title：Biotechnology and bioengineering

authors： Arnold RG,Olson TM,Hoffmann MR

更新日期：1986-11-01 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...

journal_title：Biotechnology and bioengineering

authors： Zheng ZM,Xu YZ,Liu HJ,Guo NN,Cai ZZ,Liu DH

更新日期：2008-08-01 00:00:00

abstract：:A comparative study of antimicrobial activity is done using three different electrospun nanofibers namely-CA, PAN, and PVC used as control and with various amounts of AgNO(3) being treated with UV-irradiation leading to the enhancement of silver nanoparticles. DMF is used as the common solvent which helps to undergo s...

journal_title：Biotechnology and bioengineering

authors： Lala NL,Ramaseshan R,Bojun L,Sundarrajan S,Barhate RS,Ying-Jun L,Ramakrishna S

更新日期：2007-08-15 00:00:00

abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR) system-associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering...

journal_title：Biotechnology and bioengineering

authors： Pazmiño-Ibarra V,Mengual-Martí A,Targovnik AM,Herrero S

更新日期：2019-11-01 00:00:00

abstract：:High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...

journal_title：Biotechnology and bioengineering

authors： Halalipour A,Duff MR Jr,Howell EE,Reyes-De-Corcuera JI

更新日期：2017-03-01 00:00:00

abstract：:Antimicrobial peptides (AMPs) kill microbes by non-specific membrane permeabilization, making them ideal templates for designing novel peptide-based antibiotics that can combat multi-drug resistant pathogens. For maximum efficacy in vivo and in vitro, AMPs must be biocompatible, salt-tolerant and possess broad-spectru...

journal_title：Biotechnology and bioengineering

authors： Saravanan R,Li X,Lim K,Mohanram H,Peng L,Mishra B,Basu A,Lee JM,Bhattacharjya S,Leong SS

更新日期：2014-01-01 00:00:00

abstract：:Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to re...

journal_title：Biotechnology and bioengineering

authors： Wu-Fienberg Y,Moore AM,Marquardt LM,Newton P,Johnson PJ,Mackinnon SE,Sakiyama-Elbert SE,Wood MD

更新日期：2014-09-01 00:00:00

abstract：:Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separate...

journal_title：Biotechnology and bioengineering

authors： Adachi M,Yamazaki M,Harada M,Shioi A,Katch S

更新日期：1997-02-20 00:00:00

abstract：:Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

journal_title：Biotechnology and bioengineering

authors： Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

更新日期：2020-05-01 00:00:00

abstract：:Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

journal_title：Biotechnology and bioengineering

authors： Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

更新日期：2017-10-01 00:00:00

abstract：:Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

journal_title：Biotechnology and bioengineering

authors： Du L,Gao R,Forster AC

更新日期：2009-12-15 00:00:00

abstract：:A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...

journal_title：Biotechnology and bioengineering

authors： Peretti SW,Bailey JE

更新日期：1987-02-01 00:00:00

abstract：:The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

journal_title：Biotechnology and bioengineering

authors： Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

更新日期：2018-09-01 00:00:00

abstract：:A kinetic model for the agglomeration of milk micelles following kappa-casein hydrolysis is described. The key features of the model are: (1) the surface potential of casein micelles is sufficient to explain the colloidal stability of the milk system; (2) the reduction in surface potential following kappa-casein hydro...

journal_title：Biotechnology and bioengineering

authors： Carlson A,Hill CG Jr,Olson NF

更新日期：1987-04-01 00:00:00

abstract：:Microbial secondary metabolites are one of the sources of therapeutic molecules in the pharmaceutical industry. Product quality and high yields of secondary metabolites are the main goals for the commercial success of a fermentation process. Our novel approach was based on the decision-tree algorithm to determine the ...

journal_title：Biotechnology and bioengineering

authors： Bradamante S,Barenghi L,Beretta G,Bonfa' M,Rollini M,Manzoni M

更新日期：2002-12-05 00:00:00

abstract：:Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

journal_title：Biotechnology and bioengineering

authors： Shen C,Meng Q,Zhang G

更新日期：2013-08-01 00:00:00

abstract：:Hydraulic characteristics of biological wastewater treatment systems were shown to affect bacterial state distributions and system performance through mathematical simulations. The term "state" is used here to mean the microbial storage product and biomass content of a bacterium. The traditional approach to simulating...

journal_title：Biotechnology and bioengineering

authors： Schuler AJ

更新日期：2006-08-05 00:00:00

abstract：:The aim of the present study is to demonstrate the use of controlled bioreactors for toxicological studies. As a model system the effect of linoleic acid on hybridoma cells is studied in two well-controlled continuously operated bioreactors placed in series. In the first reactor the effect on rapid proliferating cells...

journal_title：Biotechnology and bioengineering

authors： Kisztelinski D,Alink GM,Rietjens IM,Bielecki S,Tramper J,Martens DE

更新日期：2006-10-20 00:00:00

abstract：:At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic substrates is important for understanding how biomass pore size distributio...

journal_title：Biotechnology and bioengineering

authors： Yang D,Moran-Mirabal JM,Parlange JY,Walker LP

更新日期：2013-11-01 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00