A cybernetic view of microbial growth: modeling of cells as optimal strategists.

abstract：:Enzyme engineering is a powerful tool to fine-tune the enzymes. It is a technique by which the stability, activity, and specificity of the enzymes can be altered. The characteristic properties of an enzyme can be amended by immobilization and protein engineering. Among them, protein engineering is the most promising, ...

journal_title：Biotechnology and bioengineering

authors： Ali M,Ishqi HM,Husain Q

更新日期：2020-06-01 00:00:00

abstract：:Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...

journal_title：Biotechnology and bioengineering

authors： Tang IC,Okos MR,Yang ST

更新日期：1989-10-20 00:00:00

abstract：:This study examined the hydrodynamic characteristics of a liquid-solid fluidized-bed bioreactor using elastic particles (PVA gel beads) of various diameters as carriers. The drag coefficient-Reynolds number, velocity-voidage, and expansion index-Reynolds number relationships observed during fluidization of PVA gel bea...

journal_title：Biotechnology and bioengineering

authors： Wu JY,Chen KC,Chen CT,Hwang SC

更新日期：2003-09-05 00:00:00

abstract：:A novel deuterium ((2)H) NMR technique as developed for measuring the total number of deuterons exchanged by lyophilised protein samples following hydrogen-deuterium (H-D) exchange. Using this methodology differences in the H-D exchange behaviour of the proteolytic enzyme subtilisin Carlsberg hydrated either in air or...

journal_title：Biotechnology and bioengineering

authors： Hutcheon GA,Parker MC,Moore BD

更新日期：2000-11-05 00:00:00

abstract：:Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

journal_title：Biotechnology and bioengineering

authors： Nandi R,Dey S,Sengupta S

更新日期：2001-11-20 00:00:00

abstract：:The production and specific productivity of hepatitis B surface antigen (HBsAg) in recombinant Chinese hamster ovary (CHO) cells were increased by 81% and threefold, respectively, when supplemented with 1.5% dimethyl sulfoxide (DMSO) in the culture medium. To investigate the mechanism of DMSO effect on HBsAg productio...

journal_title：Biotechnology and bioengineering

authors： Wang W,Yi X,Zhang Y

更新日期：2007-06-15 00:00:00

abstract：:The sustainability of autotrophic granular system performing partial nitritation and anaerobic ammonium oxidation (anammox) for complete nitrogen removal is impaired by the production of nitrous oxide (N2 O). A systematic analysis of the pathways and affecting parameters is, therefore, required for developing N 2 O mi...

journal_title：Biotechnology and bioengineering

authors： Chen X,Ni BJ,Sin G

更新日期：2019-06-01 00:00:00

abstract：:The performance and microbial communities of three differently inoculated biotrickling filters removing dimethyl sulfide (DMS) were compared. The biotrickling filters were inoculated with Thiobacillus thioparus TK-m (THIO), sludge (HANDS) and sludge + T. thioparus TK-m + Hyphomicrobium VS (HANDS++), respectively. The ...

journal_title：Biotechnology and bioengineering

authors： Sercu B,Boon N,Beken SV,Verstraete W,Van Langenhove H

更新日期：2007-03-01 00:00:00

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...

journal_title：Biotechnology and bioengineering

authors： Yamada R,Wakita K,Mitsui R,Ogino H

更新日期：2017-09-01 00:00:00

abstract：:Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy i...

journal_title：Biotechnology and bioengineering

authors： Martín Pérez L,Benítez Casanova L,Moreno Pérez AJ,Pérez Gómez D,Gavaldá Martín S,Ledesma-García L,Valbuena Crespo N,Díez García B,Reyes-Sosa FM

更新日期：2017-11-01 00:00:00

abstract：:A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the p...

journal_title：Biotechnology and bioengineering

authors： Chung BH,Park KS

更新日期：1998-01-20 00:00:00

abstract：:A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

journal_title：Biotechnology and bioengineering

authors： Corchero JL,Villaverde A

更新日期：1998-06-20 00:00:00

abstract：:Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA...

journal_title：Biotechnology and bioengineering

authors： Ino K,Kawasumi T,Ito A,Honda H

更新日期：2008-05-01 00:00:00

abstract：:The enzymology and kinetics of tyrosine phenol lyase (TPL) from Erwinia herbicola, and tyrosine decarboxylase (TDC) from Streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. In this multienzyme biotransformation using whole c...

journal_title：Biotechnology and bioengineering

authors： Anderson WA,Moo-Young M,Legge RL

更新日期：1992-03-25 00:00:00

abstract：:The majority of influenza vaccines are manufactured using embryonated hens' eggs. The potential occurrence of a pandemic outbreak of avian influenza might reduce or even eliminate the supply of eggs, leaving the human population at risk. Also, the egg-based production technology is intrinsically cumbersome and not eas...

journal_title：Biotechnology and bioengineering

authors： George M,Farooq M,Dang T,Cortes B,Liu J,Maranga L

更新日期：2010-08-15 00:00:00

abstract：:A means of rapidly renaturing denatured protein was devised and evaluated. Three liquids were laminarly layered in a centrifuge tube, in which two solutions sandwiched liquid paraffin so as to form a pseudolipid bilayer. Denatured and aggregted protein placed on the upper surface of liquid paraffin was renatured as it...

journal_title：Biotechnology and bioengineering

authors： Yoshii H,Kojima T,Furuta T,Yasunishi A

更新日期：1994-01-05 00:00:00

abstract：:Hexanal is a key organoleptic element of green-note that is found in both fragrances and flavors. We report a novel process for the production of hexanal using immobilized enzyme templates extracted from different plant sources in combination with hollow-fiber ultrafiltration for in situ separation. Enzyme templates, ...

journal_title：Biotechnology and bioengineering

authors： Schade F,Thompson JE,Legge RL

更新日期：2003-11-05 00:00:00

abstract：:Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions ...

journal_title：Biotechnology and bioengineering

authors： Dismer F,Hansen S,Oelmeier SA,Hubbuch J

更新日期：2013-03-01 00:00:00

abstract：:The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...

journal_title：Biotechnology and bioengineering

authors： Büttner W,Becker M,Rupprich C,Boeden HF,Henklein P,Loth F,Dautzenberg H

更新日期：1989-01-05 00:00:00

abstract：:Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limi...

journal_title：Biotechnology and bioengineering

authors： Schoborg JA,Hershewe JM,Stark JC,Kightlinger W,Kath JE,Jaroentomeechai T,Natarajan A,DeLisa MP,Jewett MC

更新日期：2018-03-01 00:00:00

abstract：:This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...

journal_title：Biotechnology and bioengineering

authors： Pihlajaniemi V,Sipponen MH,Pastinen O,Nyyssölä A,Laakso S

更新日期：2016-12-01 00:00:00

abstract：:Innovation in monoclonal antibody (mAb) production continues to be driven by cell engineering strategies to increase yield and improve product quality. In a previous study, to investigate the effectiveness of transporter overexpression strategies, we prepared a taurine transporter-overexpressing host cell line (DXB11/...

journal_title：Biotechnology and bioengineering

authors： Tabuchi H,Sugiyama T

更新日期：2013-08-01 00:00:00

abstract：:Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

journal_title：Biotechnology and bioengineering

authors： Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

更新日期：1991-11-01 00:00:00

abstract：:Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...

journal_title：Biotechnology and bioengineering

authors： Coelho NM,Rodrigues AA,Arroja LM,Capela IF

更新日期：2007-02-01 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:This paper describes construction of a nanosensory device for amplified detection of biologically important amines as chemical signals. The device was inspired by a biological signal transduction system, and was fabricated on an artificial cell membrane through self-organization of the molecular components, such as a ...

journal_title：Biotechnology and bioengineering

authors： Sasaki Y,Shioyama Y,Tian WJ,Kikuchi J,Hiyama S,Moritani Y,Suda T

更新日期：2010-01-01 00:00:00

abstract：:Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

journal_title：Biotechnology and bioengineering

authors： Ostermeier M

更新日期：2003-06-05 00:00:00

abstract：:Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are ...

journal_title：Biotechnology and bioengineering

authors： Sekelsky AM,Shreve GS

更新日期：1999-05-20 00:00:00

abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...

journal_title：Biotechnology and bioengineering

authors： Gianfreda L,Toscano G,Pirozzi D,Greco G Jr

更新日期：1991-12-05 00:00:00