Production of Bacillus thuringiensis spores in total cell retention culture and two-stage continuous culture using an internal ceramic filter system.

abstract：:Growth of microorganisms on substitutable substrate mixtures display diverse growth dynamics characterized by simultaneous or preferential uptake of carbon sources. This article shows that cybernetic modeling concepts which were successful in predicting diauxic growth patterns can be extended to describe simultaneous ...

journal_title：Biotechnology and bioengineering

authors： Ramakrishna R,Ramkrishna D,Konopka AE

更新日期：1996-10-05 00:00:00

abstract：:The aim of the present study is to demonstrate the use of controlled bioreactors for toxicological studies. As a model system the effect of linoleic acid on hybridoma cells is studied in two well-controlled continuously operated bioreactors placed in series. In the first reactor the effect on rapid proliferating cells...

journal_title：Biotechnology and bioengineering

authors： Kisztelinski D,Alink GM,Rietjens IM,Bielecki S,Tramper J,Martens DE

更新日期：2006-10-20 00:00:00

abstract：:Lentiviral vectors (LVs) enveloped with an engineered Sindbis virus glycoprotein can specifically bind to dendritic cells (DCs) through the surface receptor DC-SIGN and induce antigen expression, thus providing an efficient method for delivering DC-directed vaccines. In this study, we constructed a stable producer lin...

journal_title：Biotechnology and bioengineering

authors： Lee CL,Chou M,Dai B,Xiao L,Wang P

更新日期：2012-06-01 00:00:00

abstract：:A model previously developed to characterize enzymatic in activation behavior was used to explain the non-first-order biphasic and grace period phenomena that are often observed with oligomeric enzymes. Luciferase and urease were used as model enzyme such as luciferase, the oligomer initially dissociates reversibly in...

journal_title：Biotechnology and bioengineering

authors： Lencki RW,Arul J,Neufeld RJ

更新日期：1992-12-20 00:00:00

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

journal_title：Biotechnology and bioengineering

authors： Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

更新日期：2020-03-01 00:00:00

abstract：:The growth dynamics of a methanol utilizing bacterium, L3, in batch bioreactors were experimentally investigated. Formaldehyde, a key intermediate of methanol oxidation, is indicated to have a significant role in the complex batch growth behavior of L3. The intricate batch growth dynamics of many microorganisms can be...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Lim HC

更新日期：1984-11-01 00:00:00

abstract：:Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...

journal_title：Biotechnology and bioengineering

authors： Greenwood AA,Farrell TW,O'Hara IM

更新日期：2013-12-01 00:00:00

abstract：:A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

journal_title：Biotechnology and bioengineering

authors： Pathi B,Kinsey ST,Locke BR

更新日期：2011-08-01 00:00:00

abstract：:The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...

journal_title：Biotechnology and bioengineering

authors： Junker B

更新日期：2001-07-05 00:00:00

abstract：:The calculation of many derived fermentation variables such as the respiratory quotient (RQ) and mass transfer coefficient (K(L)a) requires the differences between the molar percentages of oxygen and carbon dioxide in the fermentor inlet and exit gas, called the %OUR and %CER. Noise and bias in %CER data is of order t...

journal_title：Biotechnology and bioengineering

authors： Royce PN,Thornhill NF

更新日期：1992-08-01 00:00:00

abstract：:A major challenge when designing cell scaffolds for chondrocyte delivery in vivo is creating scaffolds with sufficient mechanical properties to restore initial function while simultaneously controlling temporal changes in the gel structure to facilitate tissue formation. To address this design challenge, degradable ph...

journal_title：Biotechnology and bioengineering

authors： Bryant SJ,Bender RJ,Durand KL,Anseth KS

更新日期：2004-06-30 00:00:00

abstract：:The bioconversion of octanoic acid into 2-heptanone by spores of Penicillium roquefortii is performed using a fed-batch technique with pH control by addition of the liquid substrate itself. The early stage of this process takes place with a high bioconversion rate and high yield. These values then decrease as a result...

journal_title：Biotechnology and bioengineering

authors： Larroche C,Besson I,Gros JB

更新日期：1994-09-05 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

journal_title：Biotechnology and bioengineering

authors： Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

更新日期：1984-09-01 00:00:00

abstract：:Various structure-similar plant secondary metabolites like ginseng saponins (ginsenosides) possess different or even totally opposite biological activities. Intentional manipulation of the ginsenoside heterogeneity in cellular biosynthesis is of great interest and significance [Zhong and Yue (2005); Adv Biochem Eng Bi...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhou X,Zhong JJ

更新日期：2008-08-01 00:00:00

abstract：:Intracellular metabolite concentration and enzyme activity measurements were made to explain the new metabolic and growth phenomena seen in the micro-aerobic, continuous yeast cultures described in Part I. The results of these assays suggested mechanisms for the observed maximum in the specific ethanol productivity as...

journal_title：Biotechnology and bioengineering

authors： Grosz R,Stephanopoulos G

更新日期：1990-12-05 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...

journal_title：Biotechnology and bioengineering

authors： Sunasara KM,Xia F,Gronke RS,Cramer SM

更新日期：2003-05-05 00:00:00

abstract：:In vivo kinetics of Saccharomyces cerevisiae are studied, in a time window of 150 s, by analyzing the response of O(2) and CO(2) in the fermentor off-gas after perturbation of chemostat cultures by metabolite pulses. Here, a new mathematical method is presented for the estimation of the in vivo oxygen uptake rate (OUR...

journal_title：Biotechnology and bioengineering

authors： Wu L,Lange HC,Van Gulik WM,Heijnen JJ

更新日期：2003-02-20 00:00:00

abstract：:Self-cycling fermentation (SCF), a cyclical, semi-continuous process that induces cell synchrony, was incorporated into a recombinant protein production scheme. Escherichia coli CY15050, a lac(-) mutant lysogenized with temperature-sensitive phage λ modified to over-express β-galactosidase, was used as a model system....

journal_title：Biotechnology and bioengineering

authors： Storms ZJ,Brown T,Sauvageau D,Cooper DG

更新日期：2012-09-01 00:00:00

abstract：:We investigated the role of green sulfur bacteria inlight-responsive electricity generation in microbial electrochemical cells (MXCs). We operated MXCs containing either monocultures or defined cocultures of previously enriched phototrophic Chlorobium and anode-respiring Geobacter under anaerobic conditions in the abs...

journal_title：Biotechnology and bioengineering

authors： Badalamenti JP,Torres CI,Krajmalnik-Brown R

更新日期：2014-02-01 00:00:00

abstract：:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

journal_title：Biotechnology and bioengineering

authors： Seo JH,Bailey JE

更新日期：1985-02-01 00:00:00

abstract：:Two methods employing a water-soluble carbodiimide for carboxyl activation were investigated for the immobilization of biochemicals to succinamidopropyl-porous glass beads. Immobilization using the simultaneous method (simultaneous addition of carbodiimide and nucleophilic ligand to the beads) and large excess of carb...

journal_title：Biotechnology and bioengineering

authors： Janolino VG,Swaisgood HE

更新日期：1982-05-01 00:00:00

abstract：:A computer-aided methodology is developed for on-line monitoring and control of cell growth in fed-batch penicillin fermentation using a semidefined medium containing low corn steep liquor concentration (5.7 g/L). Cell growth is monitored and controlled with the use of experimental correlation and carbon-balancing equ...

journal_title：Biotechnology and bioengineering

authors： Mou DG,Cooney CL

更新日期：1983-01-01 00:00:00

abstract：:The enantiocatalytic performance of immobilized lipase in an emulsion membrane reactor using stable emulsion prepared by membrane emulsification technology was studied. The production of optical pure (S)-naproxen from racemic naproxen methyl ester was used as a model reaction system. The O/W emulsion, containing the s...

journal_title：Biotechnology and bioengineering

authors： Giorno L,Li N,Drioli E

更新日期：2003-12-20 00:00:00

abstract：:The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain...

journal_title：Biotechnology and bioengineering

authors： Meireles LA,Guedes AC,Malcata FX

更新日期：2003-01-05 00:00:00

abstract：:Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...

journal_title：Biotechnology and bioengineering

authors： Kim DM,Kim HS

更新日期：1992-02-05 00:00:00

abstract：:Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...

journal_title：Biotechnology and bioengineering

authors： Ibbotson PG,Spedding PL,Otterburn MS

更新日期：1990-10-05 00:00:00

abstract：:Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

journal_title：Biotechnology and bioengineering

authors： Martínez-Alonso M,García-Fruitós E,Villaverde A

更新日期：2008-12-15 00:00:00