Abstract:
:The application of the unscented Kalman filter to control starvation-induced programmed cell death-apoptosis-in Chinese hamster ovary cells was investigated. Neural network-based sensitivity analysis identified glutamine and asparagine as two major amino acids that play a key role in the suppression of apoptosis. Dynamic equations that accounted for the dependence of apoptotic cells on the concentrations of viable cells, glutamine, and asparagine were derived. These state equations were highly nonlinear and included nine state variables. An oxygen mass balance was written in the liquid phase. It served as the output equation for the unscented Kalman filter. Using the oxygen uptake rate as the observer, it was possible to estimate the states. A model predictive controller was then implemented once the apoptotic cells in the bioreactor approached a concentration of 1.5 x 10(4) cells/mL, taking into account the operating range of the flow cytometer and measurement error. The manipulated variables were the flow rates of glucose, glutamine, and asparagine. Simulation results showed that the controller was able to keep the apoptotic cells at a concentration of 1.5 x 10(4) cells/mL.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Simon L,Karim MNdoi
10.1002/bit.10250subject
Has Abstractpub_date
2002-06-20 00:00:00pages
645-57issue
6eissn
0006-3592issn
1097-0290journal_volume
78pub_type
杂志文章abstract::A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of cu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10111
更新日期:2002-01-20 00:00:00
abstract::The anaerobic oxidation of ammonium (anammox) uses nitrite as terminal electron acceptor. The nitrite can cause inhibition to the bacteria that catalyze the anammox reaction. The literature shows a great divergence on the levels of NO2 (-) causing inhibition. Moreover, the conditions influencing the resistance of anam...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25269
更新日期:2014-10-01 00:00:00
abstract::Increases in our understanding of gene function have greatly expanded the repertoire of possible genetic interventions at our disposal with the consequence that many genetic engineering applications require multiple manipulations in which target genes can be both overexpressed and silenced in a simple and co-ordinated...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21303
更新日期:2007-04-01 00:00:00
abstract::The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980320)57:6<704::aid-bit
更新日期:1998-03-20 00:00:00
abstract::Many bioprocesses depend on the effective formation of a biofilm on a solid support. In the present study, three different surface treatments (sandblasting, pure-O(2) plasma, and He-O(2) plasma treatments) were conducted on polypropylene (PP) Pall rings used as a support in biotrickling filters for air pollution contr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22354
更新日期:2009-08-15 00:00:00
abstract::Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase G(1). Although this hypothesis ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260421109
更新日期:1993-12-01 00:00:00
abstract::Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24527
更新日期:2012-10-01 00:00:00
abstract::The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate-butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and h...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961105)52:3<404::AID-BIT
更新日期:1996-11-05 00:00:00
abstract::We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...
journal_title:Biotechnology and bioengineering
pub_type: 信件
doi:10.1002/bit.23181
更新日期:2011-10-01 00:00:00
abstract::Autotrophic or mixotrophic use of one-carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO2 and formate, genes coding for glycine synthase system from Gottschalkia acidurici were...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27658
更新日期:2020-12-17 00:00:00
abstract::Neural stem cells (NSCs) are undifferentiated, primitive cells with important potential applications including the replacement of neural tissue lost due to neurodegenerative diseases, including Parkinson's disease, as well as brain and spinal cord injuries, including stroke. We have developed methods to rapidly expand...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20246
更新日期:2004-11-05 00:00:00
abstract::Pronounced spatial nonuniformities in cell density, physiology, and activity frequently arise within densely packed immobilized cell supports. For a more fundamental understanding of immobilized cell phenomena, we have developed high-resolution microfluorimetric procedures to analyze local variations in both immobiliz...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380404
更新日期:1991-08-05 00:00:00
abstract::This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21823
更新日期:2008-08-01 00:00:00
abstract::A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260351107
更新日期:1990-05-01 00:00:00
abstract::This article compares the processes for wheat straw lignocellulose fractionation by percolation, counter-current progressing batch percolation and batch reaction at low NaOH-loadings (3-6% of DM). The flow-through processes were found to improve delignification and subsequent enzymatic saccharification, reduce NaOH-co...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26030
更新日期:2016-12-01 00:00:00
abstract::A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300604
更新日期:1987-10-20 00:00:00
abstract::Current human gene therapy relies on genetic modification of the patient's own cells. An alternate nonautologous approach is to use universal cell lines engineered to secrete therapeutic products. Protection with immunoisolation devices before implantation would allow the use of the same recombinant cell line for trea...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960805)51:3<271::AID-BIT
更新日期:1996-08-05 00:00:00
abstract::In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340613
更新日期:1989-09-01 00:00:00
abstract::A major challenge when designing cell scaffolds for chondrocyte delivery in vivo is creating scaffolds with sufficient mechanical properties to restore initial function while simultaneously controlling temporal changes in the gel structure to facilitate tissue formation. To address this design challenge, degradable ph...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20160
更新日期:2004-06-30 00:00:00
abstract::Rare sugars have various known biological functions and potential for applications in pharmaceutical, cosmetics, and food industries. Here we designed and constructed a recombination pathway in Corynebacterium glutamicum, in which dihydroxyacetone phosphate (DHAP), an intermediate of the glycolytic pathway, and a vari...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25345
更新日期:2015-01-01 00:00:00
abstract::The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22688
更新日期:2010-06-01 00:00:00
abstract::A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packedbed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethano...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270907
更新日期:1985-09-01 00:00:00
abstract::Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420207
更新日期:1993-06-20 00:00:00
abstract::By investigating the effects of four operating variables-volume (V), Ultrafiltration flux (J), enzyme concentration (E), and substrate concentration (S)-on capacity (K) and conversion rate (epsilon) of a hollow fiber CSTR, the performances of the CSTR and the kinetic constants of the reaction were determined. A model ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310704
更新日期:1988-05-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approache...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.27462
更新日期:2020-10-01 00:00:00
abstract::To manufacture biotechnology products, mammalian or bacterial cells are engineered for the production of recombinant therapeutic human proteins including monoclonal antibodies. Host cells synthesize an entire repertoire of proteins which are essential for their own function and survival. Biotechnology manufacturing pr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25647
更新日期:2015-11-01 00:00:00
abstract::A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26249
更新日期:2017-07-01 00:00:00
abstract::MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25539
更新日期:2015-07-01 00:00:00
abstract::Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitab...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26534
更新日期:2018-05-01 00:00:00
abstract::At the laboratory scale, sudden step increases from 30 to 42 degrees C can be readily accomplished when expressing heterologous proteins in heat-inducible systems. However, for large scale-cultures only slow ramp-type increases in temperature are possible due to heat transfer limitations, where the heating rate decrea...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22084
更新日期:2009-02-01 00:00:00