Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter.

abstract：:Effective enzyme-mediated viscosity reduction, disaggregation, or "liquefaction," is required to overcome the rheological challenges resulting from the fibrous, hygroscopic nature of lignocellulosic biomass, particularly at the high solids loadings that will be required for an economically viable process. However, the...

journal_title：Biotechnology and bioengineering

authors： van der Zwan T,Hu J,Saddler JN

更新日期：2017-11-01 00:00:00

abstract：:A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...

journal_title：Biotechnology and bioengineering

authors： Nath S,Schütte H,Hustedt H,Deckwer WD

更新日期：1993-09-20 00:00:00

abstract：:Two-dimensional image analysis was applied to counting, sizing, and density determinations of granules in full-scale and laboratory-scale upflow anaerobic sludge blanket (UASB) digesters. An advantage of this technique for monitoring laboratory-scale digester sludge is the small amount of material required for analysi...

journal_title：Biotechnology and bioengineering

authors： Dudley BT,Howgrave-Graham AR,Bruton AG,Wallis FM

更新日期：1993-07-01 00:00:00

abstract：:The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhong JJ

更新日期：2005-02-20 00:00:00

abstract：:An immobilized enzyme reactor has been developed for the degradation of bilirubin as a potential treatment for neonatal jaundice. It utilizes the enzyme bilirubin oxidase from Myrothecium verrucaria, which in the presence of molecular oxygen converts bilirubin to biliverdin and other products that are much less toxic ...

journal_title：Biotechnology and bioengineering

authors： Sung C,Lavin A,Klibanov AM,Langer R

更新日期：1986-10-01 00:00:00

abstract：:Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...

journal_title：Biotechnology and bioengineering

authors： Racine J,Cheradame H,Chu YS,Thiery JP,Rodriguez I

更新日期：2011-04-01 00:00:00

abstract：:We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

journal_title：Biotechnology and bioengineering

authors： Martins JF,Da Ponte MN,Barreiros S

更新日期：1993-08-05 00:00:00

abstract：:Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...

journal_title：Biotechnology and bioengineering

authors： Gorman LA,Dordick JS

更新日期：1992-02-20 00:00:00

abstract：:Cytidine-5'-diphosphocholine (CDP-choline) is a widely used neuroprotective drug for multiple indications. In industry, CDP-choline is synthesized by a two-step cell culture/permeabilized cell biotransformation method because substrates often do not enter cells in an efficient manner. This study develops a novel one-s...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Liu Q,Liu H,Zhou X,Zhang Y,Cai M

更新日期：2020-05-01 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:Osteochondral tissue engineering aims to regenerate functional tissue-mimicking physiological properties of injured cartilage and its subchondral bone. Given the distinct structural and biochemical difference between bone and cartilage, bilayered scaffolds, and bioreactors are commonly employed. We present an osteocho...

journal_title：Biotechnology and bioengineering

authors： Xue R,Chung B,Tamaddon M,Carr J,Liu C,Cartmell SH

更新日期：2019-11-01 00:00:00

abstract：:Constitutive activation of Wnt signaling plays an important role in colorectal and liver tumorigenesis. Cell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molec...

journal_title：Biotechnology and bioengineering

authors： Yamaguchi K,Zhu C,Ohsugi T,Yamaguchi Y,Ikenoue T,Furukawa Y

更新日期：2017-12-01 00:00:00

abstract：:Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

journal_title：Biotechnology and bioengineering

authors： Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

更新日期：2012-01-01 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:Chemostat cultivation of Aspergillus niger and other filamentous fungi is often hindered by the spontaneous appearance of morphologic mutants. Using the Variomixing bioreactor and applying different chemostat conditions we tried to optimize morphologic stability in both ammonium- and glucose-limited cultures. In most ...

journal_title：Biotechnology and bioengineering

authors： van de Vondervoort PJ,Poulsen BR,Ruijter GJ,Schuleit T,Visser J,Iversen JJ

更新日期：2004-05-05 00:00:00

abstract：:Frozen bagged seed inoculum was prepared, thawed and tested for seven cultures. Thawing techniques were developed and other key influences on thawing rate were quantified; seed bag thawing without a water bath rarely required more than 4 to 5 h and was as short as 0.5 to 1 h for lower fill volume bags. Testing include...

journal_title：Biotechnology and bioengineering

authors： Junker B,Seeley A,Lester M,Kovatch M,Schmitt J,Boryscwicz S,Lynch J,Zhang J,Greasham R

更新日期：2002-09-20 00:00:00

abstract：:A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population ...

journal_title：Biotechnology and bioengineering

authors： Daugelavicius R,Bakiene E,Berzinskiene J,Bamford DH

更新日期：2000-01-01 00:00:00

abstract：:A general framework for a genetically structured model is presented. The framework allows description of the interactions in a system of regulatory and structural genes. The model assumes equilibrium kinetics for the binding of regulatory proteins to the promoter regions of the genes and includes the possible activati...

journal_title：Biotechnology and bioengineering

authors： Agger T,Nielsen J

更新日期：1999-01-01 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/...

journal_title：Biotechnology and bioengineering

authors： Taira S,Du YZ,Hiratsuka Y,Uyeda TQ,Yumoto N,Kodaka M

更新日期：2008-02-15 00:00:00

abstract：:Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Yup Lee S

更新日期：2004-06-20 00:00:00

abstract：:Two methods employing a water-soluble carbodiimide for carboxyl activation were investigated for the immobilization of biochemicals to succinamidopropyl-porous glass beads. Immobilization using the simultaneous method (simultaneous addition of carbodiimide and nucleophilic ligand to the beads) and large excess of carb...

journal_title：Biotechnology and bioengineering

authors： Janolino VG,Swaisgood HE

更新日期：1982-05-01 00:00:00

abstract：:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...

journal_title：Biotechnology and bioengineering

authors： Miller WM,Wilke CR,Blanch HW

更新日期：1989-01-20 00:00:00

abstract：:Currently in America the biofuel ethanol is primarily being produced by the dry grind technique to obtain the starch contained in the corn grains and subsequently subjected to fermentation. This so-called 1st generation technology has two setbacks; first the lingering debate whether its life cycle contributes to a red...

journal_title：Biotechnology and bioengineering

authors： Brehmer B,Bals B,Sanders J,Dale B

更新日期：2008-09-01 00:00:00

abstract：:A novel method has been developed for the separation of bioproducts from yeast cells. The method uses a combination of physical, chemical, and biological agents such as lytic enzymes, osmotic supports, and spheroplast stabilizers. Using this technique, products (proteins and enzymes) can be released from specific cell...

journal_title：Biotechnology and bioengineering

authors： Huang RB,Andrews BA,Asenjo JA

更新日期：1991-11-01 00:00:00

abstract：:The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

journal_title：Biotechnology and bioengineering

authors： Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

更新日期：2004-09-20 00:00:00

abstract：:Cellulose fibers in water form networks that give rise to an apparent yield stress, especially at high solids contents. Measuring the yield stress and correlating it with fiber concentration is important for the biomass and pulp industries. Understanding how the yield stress behaves at high solids concentrations is cr...

journal_title：Biotechnology and bioengineering

authors： Lavenson DM,Tozzi EJ,McCarthy MJ,Powell RL

更新日期：2011-10-01 00:00:00

abstract：:Mammalian cell lines for recombinant protein production need to maintain productivity over extended cultivation times. Long-term stability studies are time and resource intensive, but are widely performed to identify and eliminate unstable candidates during cell line development. Production instability of manufacturin...

journal_title：Biotechnology and bioengineering

authors： Osterlehner A,Simmeth S,Göpfert U

更新日期：2011-11-01 00:00:00

abstract：:The placement of the reference electrode (RE) in various bioelectrochemical systems is often varied to accommodate different reactor configurations. While the effect of the RE placement is well understood from a strictly electrochemistry perspective, there are impacts on exoelectrogenic biofilms in engineered systems ...

journal_title：Biotechnology and bioengineering

authors： Zhang F,Liu J,Ivanov I,Hatzell MC,Yang W,Ahn Y,Logan BE

更新日期：2014-10-01 00:00:00

abstract：:Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assem...

journal_title：Biotechnology and bioengineering

authors： Dahodwala SK,Weibel MK,Humphrey AE

更新日期：1976-12-01 00:00:00