Abstract:
:We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) power whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. The ESEM is a remarkable new instrument that will have wide applicability in imaging of biological materials in their native environments.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Roziewski K,Russell AJdoi
10.1002/bit.260391114subject
Has Abstractpub_date
1992-05-01 00:00:00pages
1171-5issue
11eissn
0006-3592issn
1097-0290journal_volume
39pub_type
杂志文章abstract::The emissions of nitrous oxide (N(2)O) and nitric oxide (NO) from biological nitrogen removal (BNR) operations via nitrification and denitrification is gaining increased prominence. While many factors relevant to the operation of denitrifying reactors can influence N(2)O and NO emissions from them, the role of differe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22704
更新日期:2010-06-15 00:00:00
abstract::Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20537
更新日期:2005-09-20 00:00:00
abstract::A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10299
更新日期:2002-08-20 00:00:00
abstract::Hydraulic characteristics of biological wastewater treatment systems were shown to affect bacterial state distributions and system performance through mathematical simulations. The term "state" is used here to mean the microbial storage product and biomass content of a bacterium. The traditional approach to simulating...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20911
更新日期:2006-08-05 00:00:00
abstract::Cellulase was modified with amphilic copolymers made of alpha-allyl-omega-methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer....
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450411
更新日期:1995-02-20 00:00:00
abstract::Currently in America the biofuel ethanol is primarily being produced by the dry grind technique to obtain the starch contained in the corn grains and subsequently subjected to fermentation. This so-called 1st generation technology has two setbacks; first the lingering debate whether its life cycle contributes to a red...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21881
更新日期:2008-09-01 00:00:00
abstract::The rate of diffusion of serum albumin (MW 6.9 x 10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decreases with increasi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390210
更新日期:1992-01-20 00:00:00
abstract::A 3D printed ultrafiltration/diafiltration (UF/DF) module is presented allowing the continuous, simultaneous concentration of retained (bio-)molecules and reduction or exchange of the salt buffer. Differing from the single-pass UF concepts known from the literature, DF operation does not require the application of sev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27233
更新日期:2020-03-01 00:00:00
abstract::The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380212
更新日期:1991-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21457
更新日期:2007-10-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410802
更新日期:1993-04-05 00:00:00
abstract::The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM C...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27476
更新日期:2020-10-01 00:00:00
abstract::A poly(acrylic acid)-polyethylene graft copolymer was prepared and used initially to couple to acid phosphatase, using soluble carbodiimides. Yields which were quite good were obtained with CMC but not with EDAC. The copolymers was used to couple trypsin using EEDQ. Several organic solvents were investigated for the p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240610
更新日期:1982-06-01 00:00:00
abstract::We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21492
更新日期:2007-12-01 00:00:00
abstract::In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251212
更新日期:1983-12-01 00:00:00
abstract::Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10711
更新日期:2003-09-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22445
更新日期:2009-11-01 00:00:00
abstract::We have developed a new cell surface display system using a major outer membrane protein of Pseudomonas aeruginosa OprF as an anchoring motif. Pseudomonas fluorescens SIK W1 lipase gene was fused to the truncated oprF gene by C-terminal deletion fusion strategy. The truncated OprF-lipase fusion protein was successfull...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20399
更新日期:2005-04-20 00:00:00
abstract::Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260201206
更新日期:1978-12-01 00:00:00
abstract::An acetate-rich wastewater, containing 170 mg/L of total organic carbon (TOC), 13 mg/L of N, and 15 mg/L of P, was treated using the enhanced biological phosphate removal (EBPR) process operated in a sequencing batch reactor. A slight change of pH of the mixed liquor from 7.0 to 6.5 led to a complete loss of phosphate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20589
更新日期:2005-10-20 00:00:00
abstract::The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980305)57:5<610::aid-bit
更新日期:1998-03-05 00:00:00
abstract::Bacterial profile modification (BPM), a form of tertiary oil recovery, diverts water from the water-flooded high-permeability zone into the oil-bearing low-permeability zone. During field use, exopolymer-producing bacteria plug the high-permeability zone only in the immediate vicinity of the injection point (the near-...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20315
更新日期:2005-01-05 00:00:00
abstract::An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a labo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330908
更新日期:1989-04-05 00:00:00
abstract::Contactless simultaneous positioning of micrometer-sized particles in suspension (e.g., copolymer beads, living cells, silicon microparts) can be performed using ultrasound. Current devices are capable of collecting particles into planes or lines by exciting a resonance in the fluid by means of a piezoelectric transdu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21315
更新日期:2007-08-01 00:00:00
abstract::The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260470513
更新日期:1995-09-05 00:00:00
abstract::To study the influence of ammonium on an antibiotic cultivation, mass transfer measurements of ammonium through microporous hydrophobic membranes using different stripping methods were carried out and compared. The higher overall mass transfer coefficients for ammonium were obtained with an acid stripping solution com...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260351012
更新日期:1990-04-25 00:00:00
abstract::A thermodynamic model was used in this study to predict the adhesion of Phanerochaete chrysosporium INA-12 as conidiospores or mycelium to various solid carriers. Theoretical predictions were closely reflected by experimental results. Amount of immobilized mycelium was higher for hydrophobic (polypropylene and polyure...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350505
更新日期:1990-03-05 00:00:00
abstract::A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20769
更新日期:2006-05-05 00:00:00
abstract::Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27226
更新日期:2020-03-01 00:00:00
abstract::Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360706
更新日期:1990-10-05 00:00:00