The denitrification capability of cluster 1 Defluviicoccus vanus-related glycogen-accumulating organisms.


:The denitrification capability of Cluster 1 Defluviicoccus vanus-related glycogen-accumulating organisms (DvGAOs) is investigated. A sequencing batch reactor (SBR) fed with acetate as the sole carbon source was operated under alternating anaerobic-aerobic conditions to enrich Cluster 1 DvGAOs. Fluorescence in situ hybridization (FISH) showed that more than 85% of the bacterial population present in the reactor bound to the probes previously designed for Cluster 1 DvGAOs. A series of batch tests were performed to evaluate the capability of the community to reduce nitrate and nitrite. The tests were carried out both before and after the adaptation of the culture to anoxic conditions, and with both the intracellularly stored carbon and acetate as the electron donors. It was found that Cluster 1 DvGAOs were able to reduce nitrate but most likely unable to reduce nitrite. When un-adapted Cluster 1 DvGAOs were exposed to nitrate for the first time, a lag phase of approximately 4 h occurred, which was likely required for the synthesis of the necessary enzymes.


Biotechnol Bioeng


Wang X,Zeng RJ,Dai Y,Peng Y,Yuan Z




Has Abstract


2008-04-15 00:00:00












  • Yield and maintenance relations of yeast growth in the chemostat at superoptimal temperatures.

    abstract::A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Van Uden N,Madeira-Lopes A

    更新日期:1976-06-01 00:00:00

  • Engineering CHO cells to overexpress a secreted reporter protein upon induction from mouse mammary tumor virus promoter.

    abstract::The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: James RI,Elton JP,Todd P,Kompala DS

    更新日期:2000-01-20 00:00:00

  • Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase.

    abstract::Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Du L,Gao R,Forster AC

    更新日期:2009-12-15 00:00:00

  • Combinatorial evolution of phosphotriesterase toward a robust malathion degrader by hierarchical iteration mutagenesis.

    abstract::Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Luo XJ,Zhao J,Li CX,Bai YP,Reetz MT,Yu HL,Xu JH

    更新日期:2016-11-01 00:00:00

  • Nitrous oxide production in autotrophic nitrogen removal granular sludge: A modeling study.

    abstract::The sustainability of autotrophic granular system performing partial nitritation and anaerobic ammonium oxidation (anammox) for complete nitrogen removal is impaired by the production of nitrous oxide (N2 O). A systematic analysis of the pathways and affecting parameters is, therefore, required for developing N 2 O mi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chen X,Ni BJ,Sin G

    更新日期:2019-06-01 00:00:00

  • Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter.

    abstract::Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

    更新日期:2000-10-20 00:00:00

  • Hydrodynamic characteristics of immobilized cell beads in a liquid-solid fluidized-bed bioreactor.

    abstract::This study examined the hydrodynamic characteristics of a liquid-solid fluidized-bed bioreactor using elastic particles (PVA gel beads) of various diameters as carriers. The drag coefficient-Reynolds number, velocity-voidage, and expansion index-Reynolds number relationships observed during fluidization of PVA gel bea...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wu JY,Chen KC,Chen CT,Hwang SC

    更新日期:2003-09-05 00:00:00

  • Protopanaxadiol 6-hydroxylase and its role in regulating the ginsenoside heterogeneity in Panax notoginseng cells.

    abstract::Various structure-similar plant secondary metabolites like ginseng saponins (ginsenosides) possess different or even totally opposite biological activities. Intentional manipulation of the ginsenoside heterogeneity in cellular biosynthesis is of great interest and significance [Zhong and Yue (2005); Adv Biochem Eng Bi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yue CJ,Zhou X,Zhong JJ

    更新日期:2008-08-01 00:00:00

  • Use of exogenous specialised bacteria in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in slurry phase conditions

    abstract::The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fava F,Bertin L

    更新日期:1999-07-20 00:00:00

  • Evaluation of selectivity changes in HIC systems using a preferential interaction based analysis.

    abstract::It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xia F,Nagrath D,Garde S,Cramer SM

    更新日期:2004-08-05 00:00:00

  • Alginate microparticles as novel carrier for oral insulin delivery.

    abstract::Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

    更新日期:2007-04-01 00:00:00

  • Continuous hydrolysis of oil by immobilized lipase in a countercurrent reactor.

    abstract::Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kosugi Y,Tanaka H,Tomizuka N

    更新日期:1990-09-01 00:00:00

  • Live reporting for hypoxia: Hypoxia sensor-modified mesenchymal stem cells as in vitro reporters.

    abstract::Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce areas of hypoxia in the tissue/matrix. However, reliable systems to detect oxygen gradients an...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schmitz C,Pepelanova I,Seliktar D,Potekhina E,Belousov VV,Scheper T,Lavrentieva A

    更新日期:2020-07-15 00:00:00

  • Ethanol production from nonsterilized carob pod extract by free and immobilized Saccharomyces cerevisiae cells using fed-batch culture.

    abstract::The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed-batch culture was investigated. Fed-batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed-batch culture, both free and immobilized S. cerev...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Roukas T

    更新日期:1994-02-05 00:00:00

  • Influence of power supply in the feasibility of Phaeodactylum tricornutum cultures.

    abstract::The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

    更新日期:2004-09-20 00:00:00

  • Alteration of bacterial surface electrostatic potential and pH upon adhesion to a solid surface and impacts to cellular bioenergetics.

    abstract::In our previous study [Hong Y, Brown DG (2009) Appl Environ Microbiol 75(8):2346-2353], the adenosine triphosphate (ATP) level of adhered bacteria was observed to be 2-5 times higher than that of planktonic bacteria. Consequently, the proton motive force (Delta p) of adhered bacteria was approximately 15% greater than...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hong Y,Brown DG

    更新日期:2010-04-01 00:00:00

  • General theory of determining intraparticle active immobilized enzyme distribution and rate parameters.

    abstract::A general theory is presented in this article for determining the intrinsic rate constants for the main reaction and deactivation reaction, the effective diffusivity of the substrate, and the active enzyme distribution within porous solid supports from deactivation study of a continuous stirred-basket reactor (CSBR). ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hossain MM,Do DD

    更新日期:1989-03-01 00:00:00

  • Diffusion of sucrose and yohimbine in calcium alginate gel beads with or without entrapped plant cells.

    abstract::The diffusion characteristics of sucrose, a nutrient, and yohimbine, a secondary metabolite, in alginate gel beads, with or without entrapped periwinkle (Catharanthus roseus) or apple (Malus domestica) cells, were investigated. Effective diffusivities of both solutes in the gel beads were determined by two different m...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pu HT,Yang RY

    更新日期:1988-09-20 00:00:00

  • Analysis of noise and bias in fermentation oxygen uptake rate data.

    abstract::The calculation of many derived fermentation variables such as the respiratory quotient (RQ) and mass transfer coefficient (K(L)a) requires the differences between the molar percentages of oxygen and carbon dioxide in the fermentor inlet and exit gas, called the %OUR and %CER. Noise and bias in %CER data is of order t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Royce PN,Thornhill NF

    更新日期:1992-08-01 00:00:00

  • Redox control bioreactor: a unique biological water processor.

    abstract::The redox control bioreactor (RCB) is a new hollow fiber membrane bioreactor (HFMBR) design in which oxygen and hydrogen gases are provided simultaneously through separate arrays of juxtaposed hollow fiber (HF) membranes. This study applied the RCB for completely autotrophic conversion of ammonia to N(2) through nitri...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Smith DP,Rector T,Reid-Black K,Hummerick M,Strayer R,Birmele M,Roberts MS,Garland JL

    更新日期:2008-03-01 00:00:00

  • Binding affinities/avidities of antibody-antigen interactions: quantification and scale-up implications.

    abstract::Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhang H,Williams PS,Zborowski M,Chalmers JJ

    更新日期:2006-12-05 00:00:00

  • Biofabrication of soft tissue templates for engineering the bone-ligament interface.

    abstract::Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Harris E,Liu Y,Cunniffe G,Morrissey D,Carroll S,Mulhall K,Kelly DJ

    更新日期:2017-10-01 00:00:00

  • On the organic solvent and thermostability of the biocatalytic redox system of Rhodococcus ruber DSM 44541.

    abstract::The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stampfer W,Kosjek B,Kroutil W,Faber K

    更新日期:2003-03-30 00:00:00

  • Apparent zero-order kinetics of phenol biodegradation by substrate-inhibited microbes at low substrate concentrations.

    abstract::The reaction kinetics for phenol biodegradation at low substrate concentrations can be estimated based on the analysis of changes in the dissolved oxygen concentration in the bulk liquid during biodegradation. The measured oxygen concentration changes with an interesting behavior as biodegradation proceeds. The oxygen...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shishido M,Toda M

    更新日期:1996-06-20 00:00:00

  • Bioartificial kidney. I. Theoretical analysis of convective flow in hollow fiber modules: application to a bioartificial hemofilter.

    abstract::Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Moussy Y

    更新日期:2000-04-20 00:00:00

  • Growth of Trichosporon cutaneum under oxygen limitation: kinetics of oxygen uptake.

    abstract::The relationship between oxygen concentration and growth rate in the yeast Trichosporon cutaneum was studied. In order to establish the conditions for purely oxygen-limited growth, the cells were first grown in a carbon-limited chemostat, and kinetic parameters determined. The cells were then grown in an oxygen-limite...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Käppeli O,Fiechter A

    更新日期:1982-11-01 00:00:00

  • On the estimation of average shear rate in bubble columns.

    abstract::Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nishikawa M

    更新日期:1991-03-25 00:00:00

  • New tools for mass isotopomer data evaluation in (13)C flux analysis: mass isotope correction, data consistency checking, and precursor relationships.

    abstract::13C metabolic flux analysis (MFA) is based on carbon-labeling experiments where a specifically (13)C labeled substrate is fed. The labeled carbon atoms distribute over the metabolic network and the label enrichment of certain metabolic pools is measured by using different methods. Recently, MS methods have been dramat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wahl SA,Dauner M,Wiechert W

    更新日期:2004-02-05 00:00:00

  • Modeling biocide action against biofilms.

    abstract::A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stewart PS,Hamilton MA,Goldstein BR,Schneider BT

    更新日期:1996-02-20 00:00:00

  • On-line gas analysis in animal cell cultivation: I. Control of dissolved oxygen and pH.

    abstract::To monitor gas reaction rates in animal cell culture at constant dissolved oxygen concentration (DO) and constant pH it was necessary to develop improved control methods. Decoupling of both controllrs was obtained by manipulation of molar fractions of oxygen and carbon dioxide in the gas phase. Two pairs of DO and pH ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Oeggerli A,Eyer K,Heinzle E

    更新日期:1995-01-05 00:00:00