Abstract:
:There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pipeline. However, in many of these proposed continuous processing model systems, viral safety has not been comprehensively addressed. Viral safety and detection is a highly important and often expensive regulatory requirement for any new biological product. To ensure success in the adaption of continuous processing to large-scale production, there is a need to consider the development of approaches that allow for seamless incorporation of viral testing and clearance/inactivation methods. In this review, we outline potential strategies to apply current viral testing and clearance/inactivation technologies to continuous processing, as well as modifications of existing unit operations to ensure the successful integration of viral clearance into the continuous processing of biological products. Biotechnol. Bioeng. 2017;114: 21-32. © 2016 Wiley Periodicals, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Johnson SA,Brown MR,Lute SC,Brorson KAdoi
10.1002/bit.26060subject
Has Abstractpub_date
2017-01-01 00:00:00pages
21-32issue
1eissn
0006-3592issn
1097-0290journal_volume
114pub_type
杂志文章,评审abstract::Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...
journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.22309
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abstract::A cyclic fed-batch bioprocess is designed and a significant improvement of rice alpha-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media...
journal_title:Biotechnology and bioengineering
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doi:10.1002/(sici)1097-0290(19980805)59:3<379::aid-bit
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abstract::Detachment from biofilms was evaluated using a mixed culture biofilm grown on primary wastewater in a tube reactor. The growth of biofilms and the detachment of biomass from biofilms are strongly influenced by hydrodynamic conditions. In a long-term study, three biofilms were cultivated in a biofilm tube reactor. The ...
journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.10503
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abstract::Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...
journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260360706
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abstract::Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260201206
更新日期:1978-12-01 00:00:00
abstract::A new method is described for the immobilization of biologically active proteins onto several types of organic polymer beads. First, the soluble protein is modified by reaction with an excess of a hydrophobic imidoester, for example methyl 4-phenyl-butyrimidate, at ca. pH 9 and 0 degrees . Excess imidoester and side p...
journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260320514
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.22937
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abstract::Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340807
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450107
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10382
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240106
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260400105
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doi:10.1002/bit.260390103
更新日期:1992-01-05 00:00:00
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doi:10.1002/bit.260270524
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2004-09-05 00:00:00
abstract::Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguishable by differences in their surface topography. Immobilizations were per...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260220203
更新日期:1980-02-01 00:00:00
abstract::A novel, alternative intensified cell culture process comprised of a linked bioreactor system is presented. An N-1 perfusion bioreactor maintained cells in a highly proliferative state and provided a continuous inoculum source to a second bioreactor operating as a continuous-flow stirred-tank reactor (CSTR). An initia...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26985
更新日期:2019-08-01 00:00:00
abstract::Acetate accumulation is a common problem observed in aerobic high cell density Escherichia coli cultures. A previous report has hypothesized that the glyoxylate shunt is active in a low acetate producer, E. coli BL21, and inactive in a high acetate producer, JM109. To further investigate this hypothesis, we now develo...
journal_title:Biotechnology and bioengineering
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doi:
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