Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates.

abstract：:There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...

journal_title：Biotechnology and bioengineering

authors： Johnson SA,Brown MR,Lute SC,Brorson KA

更新日期：2017-01-01 00:00:00

abstract：:The study was done to evaluate the efficacy of using reuterin produced by Lactobacillus reuteri to sanitize biological tissues. The microorganism tested in the study was Pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. The inhibitory effect of reuterin on P. aeruginosa for an inocul...

journal_title：Biotechnology and bioengineering

authors： Liang HF,Chen CN,Chang Y,Sung HW

更新日期：2003-10-20 00:00:00

abstract：:Mechanical forces regulate the structure and function of many tissues in vivo; recent results indicate that the mechanical environment can decisively influence the development of engineered tissues cultured in vitro. To investigate the effects of the hydrodynamic environment on tissue-engineered cartilage, primary bov...

journal_title：Biotechnology and bioengineering

authors： Gooch KJ,Kwon JH,Blunk T,Langer R,Freed LE,Vunjak-Novakovic G

更新日期：2001-02-20 00:00:00

abstract：:The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glyc...

journal_title：Biotechnology and bioengineering

authors： Gawlitzek M,Valley U,Wagner R

更新日期：1998-03-05 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Syn...

journal_title：Biotechnology and bioengineering

authors： Agostoni M,Waters CM,Montgomery BL

更新日期：2016-02-01 00:00:00

abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

journal_title：Biotechnology and bioengineering

authors： Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

更新日期：2009-02-15 00:00:00

abstract：:Air emissions from the pulp and paper industry frequently contain reduced sulfur compounds (RSC), such as dimethyl sulfide (DMS) mixed with volatile organic compounds (VOC) (e.g., methanol, MeOH) and it is desirable to treat either one or both of these groups of compounds. The objective of this study was to assess the...

journal_title：Biotechnology and bioengineering

authors： Zhang Y,Liss SN,Allen DG

更新日期：2006-11-05 00:00:00

abstract：:Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates deri...

journal_title：Biotechnology and bioengineering

authors： Beauchesne PR,Bruce KJ,Bowen BD,Piret JM

更新日期：2010-04-15 00:00:00

abstract：:A model previously developed to characterize enzymatic in activation behavior was used to explain the non-first-order biphasic and grace period phenomena that are often observed with oligomeric enzymes. Luciferase and urease were used as model enzyme such as luciferase, the oligomer initially dissociates reversibly in...

journal_title：Biotechnology and bioengineering

authors： Lencki RW,Arul J,Neufeld RJ

更新日期：1992-12-20 00:00:00

abstract：:The microbial degradation of quinoline by Comamonas acidovorans was studied in a laboratory scale stirred tank reactor. In continuous culture experiments using quinoline as a sole source of carbon and nitrogen, it was shown by means of mass balances that quinoline was converted completely to biomass, carbon dioxide, a...

journal_title：Biotechnology and bioengineering

authors： Miethling R,Hecht V,Deckwer WD

更新日期：1993-08-20 00:00:00

abstract：:The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) of transf...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Bae SW,Jeong Hong H,Min Lee G

更新日期：1996-08-20 00:00:00

abstract：:The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...

journal_title：Biotechnology and bioengineering

authors： Janseen AE,Van der Padt A,Van Sonsbeek HM,Van't Riet K

更新日期：1993-01-05 00:00:00

abstract：:The selectivity of protein extraction by reversed micellar solutions can be improved by the addition of affinity cosurfactants bearing ligands which bind strongly to the target protein. The interactions between cosurfactant and protein, as well as the interfacial activity of both the free cosurfactant and the protein-...

journal_title：Biotechnology and bioengineering

authors： Kelley BD,Wang DI,Hatton TA

更新日期：1993-11-20 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in pr...

journal_title：Biotechnology and bioengineering

authors： Wu FJ,Friend JR,Lazar A,Mann HJ,Remmel RP,Cerra FB,Hu WS

更新日期：1996-10-05 00:00:00

abstract：:Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...

journal_title：Biotechnology and bioengineering

authors： Davidson AJ,Ellis MJ,Chaudhuri JB

更新日期：2010-08-15 00:00:00

abstract：:The accumulation of inorganic phosphate inhibits protein synthesis in cell-free protein synthesis reactions that are energized by high-energy-phosphate-containing compounds. This study developed a new scheme for supplying energy using dual energy sources to enhance the regeneration of ATP and lower the rate of phospha...

journal_title：Biotechnology and bioengineering

authors： Kim TW,Oh IS,Keum JW,Kwon YC,Byun JY,Lee KH,Choi CY,Kim DM

更新日期：2007-08-15 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:In this work the question was addressed if in nitrite-oxidizing activated sludge systems the environmental competition between Nitrobacter spp. and Nitrospira spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculate...

journal_title：Biotechnology and bioengineering

authors： Nogueira R,Melo LF

更新日期：2006-09-05 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:UV-visible spectroscopy (UV-Vis) is routinely used in microbiology as a tool to check the optical density (OD) pertaining to the growth stages of microbial cultures at the single wavelength of 600 nm, better known as the OD600 . Typically, modern UV-Vis spectrophotometers can scan in the region of approximately 200-10...

journal_title：Biotechnology and bioengineering

authors： Chapman J,Orrell-Trigg R,Kwoon KY,Truong VK,Cozzolino D

更新日期：2021-01-05 00:00:00

abstract：:PER.C6 cells were cultivated for propagation of a replication-defective adenovirus vector in serum-free suspension bioreactors. Cellular metabolism during cell growth and adenovirus propagation was fully characterized using on-line and off-line methods. The energy metabolism was found to accelerate transiently after a...

journal_title：Biotechnology and bioengineering

authors： Maranga L,Auniņs JG,Zhou W

更新日期：2005-06-05 00:00:00

abstract：:Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by t...

journal_title：Biotechnology and bioengineering

authors： Watson VJ,Logan BE

更新日期：2010-02-15 00:00:00

abstract：:The ferrous-iron oxidation kinetics of a bacterial culture consisting predominantly of Leptospirillum ferrooxidans were studied in continuous-flow bioreactors. The bacterial culture was fed with a salts solution containing 12 g/L ferrous-iron, at dilution rates ranging from 0.01 to 0.06 l/h, and temperatures ranging f...

journal_title：Biotechnology and bioengineering

authors： Breed AW,Dempers CJ,Searby GE,Gardner MN,Rawlings DE,Hansford GS

更新日期：1999-10-05 00:00:00

abstract：:Use of cellulase for denim washing is a standard eco-friendly technique to achieve desirable appearance and softness for cotton fabrics and denims. But enzymatic washing of denim till date involved acid cellulase (Trichoderma reesei) and neutral cellulase (Humicola isolens) the use of which has a drawback of backstain...

journal_title：Biotechnology and bioengineering

authors： Anish R,Rahman MS,Rao M

更新日期：2007-01-01 00:00:00

abstract：:Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and di...

journal_title：Biotechnology and bioengineering

authors： Chia SM,Lin PC,Yu H

更新日期：2005-03-05 00:00:00

abstract：:For direct and on-line study of the physiological states of cell cultures, a robust flow injection system has been designed and interfaced with flow cytometry (FI-FCM). The core of the flow injection system includes a microchamber designed for sample processing. The design of this microchamber allows not only an accur...

journal_title：Biotechnology and bioengineering

authors： Zhao R,Natarajan A,Srienc F

更新日期：1999-03-05 00:00:00

abstract：:The application of rational design in reallocating metabolic flux to accumulate desired chemicals is always restricted by the native regulatory network. In this study, recombinant Pichia pastoris was constructed for malic acid production from sole methanol through rational redistribution of metabolic flux. Different m...

journal_title：Biotechnology and bioengineering

authors： Guo F,Dai Z,Peng W,Zhang S,Zhou J,Ma J,Dong W,Xin F,Zhang W,Jiang M

更新日期：2021-01-01 00:00:00