Abstract:
:Lyophilized proteins exhibit "pH memory," i.e., their behavior in the solid form corresponds to the pH of the aqueous solution from which they were freeze dried. Herein, we directly tested whether the ionization state is "remembered" by model organic compounds containing various protein functional groups (amino, carboxyl, and phenolic). The fraction of ionized species was quantitated from the infrared spectra of both the aqueous and lyophilized states. The pK(a) values in the aqueous and lyophilized forms for each compound were found to be quite similar, within 0.3 units from each other.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Costantino HR,Griebenow K,Langer R,Klibanov AMdoi
10.1002/(SICI)1097-0290(19970205)53:3<345::AID-BITsubject
Has Abstractpub_date
1997-02-05 00:00:00pages
345-8issue
3eissn
0006-3592issn
1097-0290journal_volume
53pub_type
杂志文章abstract::This paper describes batch and semicontinuous acetic acid fermentations for wine vinegar production carried out with Acetobacter pasteurianus, and an industrial strain using a noncommercial 100-L bubble column reactor equipped with a novel type of gas-liquid dynamic sparger. Results showed acetification rates with thi...
journal_title:Biotechnology and bioengineering
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更新日期:1999-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330105
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280213
更新日期:1986-02-01 00:00:00
abstract::Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260380110
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journal_title:Biotechnology and bioengineering
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更新日期:2008-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270112
更新日期:1985-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420207
更新日期:1993-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260381016
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abstract::Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961105)52:3<404::AID-BIT
更新日期:1996-11-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260200709
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1992-10-05 00:00:00
abstract::A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
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更新日期:2002-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
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abstract::One of the major bottlenecks in the bioconversion of lignocelluosic feedstocks to liquid ethanol is the recalcitrance of residue following pretreatment, specifically softwood derived residues. Peroxide delignification has previously been shown to effectively aid in the removal of condensed lignaceous moieties from sub...
journal_title:Biotechnology and bioengineering
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更新日期:2010-08-15 00:00:00