Recovery of insect cells using hollow fiber microfiltration.

Abstract:

:An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were obtained using fibers with internal diameter of 0.75 mm, 0.45 microm pore size, and a cell suspension flow at a shear rate of 14000 s(-1) (0.032 L/min per fiber). A flux rate of 500 L/m(2) h can be obtained by maintaining the surface area/cell ratio at 0.05 m(2)/10 L of cells at a concentration of 2.5 x 10(6) cells/mL. Forty liters of infected insect cells can be concentrated 10 times in 20 min without affecting cell viability.

journal_name

Biotechnol Bioeng

authors

Trinh L,Shiloach J

doi

10.1002/bit.260480412

subject

Has Abstract

pub_date

1995-11-20 00:00:00

pages

401-5

issue

4

eissn

0006-3592

issn

1097-0290

journal_volume

48

pub_type

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