The production of cellulase in a spouted bed fermentor using cells immobilized in biomass support particles.

Abstract:

:Continuous cellulase production by Trichoderma viride QM 9123, immobilized in 6 mm diameter, spherical, stainless steel biomass support particles, has been achieved using a medium containing glucose as the main carbon source. Experiments were carried out in a 10-L spouted bed fermentor. In this type of reactor-recycled broth is used to create a jet at the base of a bed of particles, causing the particles to spout and circulate. During the circulation, particles pass through a region of high shear near the jet inlet. This effectively prevents a buildup of excess biomass and thus enables steady-state conditions to be achieved during continuous operation. Continuous production of cellulase was achieved at significantly higher yield and productivity than in conventional systems. At a dilution rate of 0.15 h(-1) (nominal washout rate for freely suspended cells is 0.012 h(-1)), the yield of cellulase on glucose was 31% higher than that measured during batch operation, while the volumetric productivity (31.5 FPA U/L. h) was 53% greater than in the batch system. The specific cellulase productivity of the immobilized cells was more than 3 times that of freely suspended cells, showing that diffusional limitations can be beneficial. This offers significant opportunity for the further development of biomass support particles and associated bioreactors.

journal_name

Biotechnol Bioeng

authors

Webb C,Fukuda H,Atkinson B

doi

10.1002/bit.260280107

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

41-50

issue

1

eissn

0006-3592

issn

1097-0290

journal_volume

28

pub_type

杂志文章
  • Increased recombinant protein production in Escherichia coli strains with overexpressed water-forming NADH oxidase and a deleted ArcA regulatory protein.

    abstract::Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20853

    authors: Vemuri GN,Eiteman MA,Altman E

    更新日期:2006-06-20 00:00:00

  • Control of the redox potential by oxygen limitation improves bacterial leaching of chalcopyrite.

    abstract::Shake flask and stirred tank bioleaching experiments showed that the dissolution of chalcopyrite is inhibited by ferric ion concentrations as low as 200 mg L(-1) and redox potentials >420 mV (vs. Ag/AgCl). Chemical leaching of chalcopyrite (4% suspension, surface area 2.3 m2 g(-1)) was enhanced fourfold in the presenc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10184

    authors: Third KA,Cord-Ruwisch R,Watling HR

    更新日期:2002-05-20 00:00:00

  • Bioaffinity purification of NADP(+)-dependent dehydrogenases: studies with alcohol dehydrogenase from Thermoanaerobacter brockii.

    abstract::This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10079

    authors: McMahon M,Mulcahy P

    更新日期:2002-03-05 00:00:00

  • Influence of mass transfer limitations on determination of the half saturation constant for hydrogen uptake in a mixed-culture CH(4)-producing enrichment.

    abstract::There is strong evidence in the literature supporting the existence of significant mass transfer limitations on the kinetics of exogenous H(2) consumption by methanogens. The half saturation constant for H (2) uptake by a mixed-culture, CH(4) producing enrichment was measured using an experimental protocol that avoide...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260400704

    authors: Giraldo-Gomez E,Goodwin S,Switzenbaum MS

    更新日期:1992-10-05 00:00:00

  • Detection and reduction of microaggregates in insulin preparations.

    abstract::Insulin is an important biotherapeutic protein, and it is also a model protein used to study amyloid diseases, such as Alzheimer's and Parkinson's. The preparation of the protein can lead to small amounts of aggregate in the solution, which in turn may lead to irreproducible in vitro results. Using several pre-treatme...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22902

    authors: Heldt CL,Sorci M,Posada D,Hirsa A,Belfort G

    更新日期:2011-01-01 00:00:00

  • Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains.

    abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10428

    authors: Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

    更新日期:2002-12-30 00:00:00

  • Anodic reactions in microbial fuel cells.

    abstract::Potentiometric and amperometric measurements were made with microbial fuel cells containing E. coli or yeast as the anodic reducing agent and glucose as the oxidizable substrate. The catalytic effects of thionine and resorufin on the anode reaction were investigated. Results on the potentiometry, polarization, and cou...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260250219

    authors: Bennetto HP,Stirling JL,Tanaka K,Vega CA

    更新日期:1983-02-01 00:00:00

  • The effect of packing hydrophilization on bacterial attachment and the relationship with the performance of biotrickling filters.

    abstract::Many bioprocesses depend on the effective formation of a biofilm on a solid support. In the present study, three different surface treatments (sandblasting, pure-O(2) plasma, and He-O(2) plasma treatments) were conducted on polypropylene (PP) Pall rings used as a support in biotrickling filters for air pollution contr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22354

    authors: Prado OJ,Popat SC,Chen G,Walker SL,Lafuente J,Gabriel D,Deshusses MA

    更新日期:2009-08-15 00:00:00

  • Overexpression of a cytosolic chaperone to improve solubility and secretion of a recombinant IgG protein in insect cells.

    abstract::The secretion of heterologous IgG proteins in the baculovirus-insect cell expression system is accompanied by substantial insoluble immunoglobulin in the infected cells. The accumulation of these insoluble forms suggests a limitation in the processing and secretory pathway of the infected cells. As a result, cytosolic...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Ailor E,Betenbaugh MJ

    更新日期:1998-04-20 00:00:00

  • Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

    abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Byk G,Soto J,Mattler C,Frederic M,Scherman D

    更新日期:1998-04-01 00:00:00

  • Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines.

    abstract::Mammalian cell lines for recombinant protein production need to maintain productivity over extended cultivation times. Long-term stability studies are time and resource intensive, but are widely performed to identify and eliminate unstable candidates during cell line development. Production instability of manufacturin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.23216

    authors: Osterlehner A,Simmeth S,Göpfert U

    更新日期:2011-11-01 00:00:00

  • Design and construction of improved new vectors for Zymomonas mobilis recombinants.

    abstract::Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.23106

    authors: Dong HW,Bao J,Ryu DD,Zhong JJ

    更新日期:2011-07-01 00:00:00

  • A model for diauxic growth.

    abstract::A model is developed to predict batch and continuous culture behavior of fermentations on two different carbon and energy sources. The basic assumption of the model is that the permease for the favored substrate is constitutive, whereas the premease for the second substrate is subject to induction and catabolite repre...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260170905

    authors: Van Dedem G,Moo-Young M

    更新日期:1975-09-01 00:00:00

  • Effects of oxygen and nutrients limitation on ajmalicine production and related enzyme activities in high density cultures of Catharanthus roseus.

    abstract::Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260440409

    authors: Schlatmann JE,Moreno PR,Vinke JL,Ten Hoopen HJ,Verpoorte R,Heijnen JJ

    更新日期:1994-08-05 00:00:00

  • Destruction and formation of toxins by one bacterial species affect biodegradation by a second species.

    abstract::Two strains of Pseudomonas able to grow on phenol or p-nitrophenol (PNP) were isolated from sewage. Pseudomonas sp. PN101 mineralized and formed nitrite from PNP but did not mineralize phenol, and Pseudomonas sp. PH111 mineralized phenol but not PNP. Phenol increased the lag period before Pseudomonas sp. PN101 grew on...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260330706

    authors: Murakami Y,Alexander M

    更新日期:1989-02-20 00:00:00

  • Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis.

    abstract::Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.22119

    authors: Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

    更新日期:2009-02-15 00:00:00

  • Surface display of MPH on Pseudomonas putida JS444 using ice nucleation protein and its application in detoxification of organophosphates.

    abstract::Methyl parathion hydrolase (MPH) has been displayed on the surface of microorganisms for the first time using only N- and C-terminal domains of the ice nucleation protein (INPNC) from Pseudomonas syringae INA5 as an anchoring motif. A shuttle vector pINCM coding for INPNC-MPH was constructed and used to target MPH ont...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21535

    authors: Yang C,Cai N,Dong M,Jiang H,Li J,Qiao C,Mulchandani A,Chen W

    更新日期:2008-01-01 00:00:00

  • Concentration-dependent internalization of a cytokine/cytokine receptor complex in human hematopoietic cells.

    abstract::Soluble steel factor (SF) is a potent stimulator of hematopoietic progenitor cell proliferation in vitro, and cytokine combinations that include SF can support extensive expansions of hematopoietic cells. Recently, we showed that very primitive progenitor cells from normal human bone marrow require exposure to very hi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:

    authors: Zandstra PW,Jervis E,Haynes CA,Kilburn DG,Eaves CJ,Piret JM

    更新日期:1999-05-20 00:00:00

  • Steam pretreatment of lignocellulosic material for enhanced enzymatic hydrolysis.

    abstract::Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism and rate of heat transfer, pentosan solu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260290213

    authors: Brownell HH,Saddler JN

    更新日期:1987-02-01 00:00:00

  • Synthesis of polyketides from low cost substrates by the thermotolerant yeast Kluyveromyces marxianus.

    abstract::Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.26976

    authors: McTaggart TL,Bever D,Bassett S,Da Silva NA

    更新日期:2019-07-01 00:00:00

  • Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.

    abstract::Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审

    doi:10.1002/bit.27269

    authors: Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

    更新日期:2020-04-01 00:00:00

  • Gelatin interpenetration in poly N-isopropylacrylamide network reduces the compressive modulus of the scaffold: A property employed to mimic hepatic matrix stiffness.

    abstract::The progression of liver disease from normal to cirrhotic state is characterized by modulation of the stiffness of the extracellular matrix (ECM). Mimicking this modulation in vitro scaffold could provide a better insight into hepatic cell behavior. In this study, interpenetrating poly(N-isopropylacrylamide-co-gelatin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.27218

    authors: Sarkar J,Kamble SC,Patil R,Kumar A,Gosavi SW

    更新日期:2020-02-01 00:00:00

  • Enzyme hydrolysis of plasma proteins in a CSTR ultrafiltration reactor: Performances and modeling.

    abstract::By investigating the effects of four operating variables-volume (V), Ultrafiltration flux (J), enzyme concentration (E), and substrate concentration (S)-on capacity (K) and conversion rate (epsilon) of a hollow fiber CSTR, the performances of the CSTR and the kinetic constants of the reaction were determined. A model ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260310704

    authors: Bressollier P,Petit JM,Julien R

    更新日期:1988-05-01 00:00:00

  • Co-immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto-oligosaccharides: Preparation, characterization and modeling.

    abstract::Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21810

    authors: Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

    更新日期:2008-07-01 00:00:00

  • Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase.

    abstract::Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260390312

    authors: Kim DM,Kim HS

    更新日期:1992-02-05 00:00:00

  • Hollow-fiber membrane bioreactors using immobilized E. coli for protein synthesis.

    abstract::Actively growing Escherichia coli C600(pBR322), immobilized within the macroporous matrix of asymmetric-wall hollow-fiber membranes, has been propagated to extremely high densities, typically more than 10(12) cells/mL of accessible void volume, in some regions cells accounting for nearly 100% of the available macrovoi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260251113

    authors: Inloes DS,Smith WJ,Taylor DP,Cohen SN,Michaels AS,Robertson CR

    更新日期:1983-11-01 00:00:00

  • On the enthalpy of formation of Escherichia coli K-12 cells.

    abstract::An examination is made of five methods for obtaining values of the enthalpy of formation of a unit mass of living Escherichia coli K-12 cells. The values obtained by these methods ranged from -88.95 kJ to -99.55 kJ, the gross average being 96.01 kJ, per unit carbon formula weight equivalent of living, hydrated cells. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260390103

    authors: Battley EH

    更新日期:1992-01-05 00:00:00

  • Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates.

    abstract::Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of serum. Existing protocols desig...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21707

    authors: Jaluria P,Konstantopoulos K,Betenbaugh M,Shiloach J

    更新日期:2008-04-15 00:00:00

  • Contribution of protein charge to partitioning in aqueous two-phase systems.

    abstract::Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating electrochemical eff...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19980820)59:4<461::aid-bit

    authors: Fan W,Bakir U,Glatz CE

    更新日期:1998-08-20 00:00:00

  • Serial flux mapping of Corynebacterium glutamicum during fed-batch L-lysine production using the sensor reactor approach.

    abstract::Using our recently developed sensor reactor approach, lysine-producing, nongrowing Corynebacterium glutamicum MH20-22B cells were subjected to serial (13)C-labeling experiments for flux analysis during the leucine-limited fed-batch production phase in a 300-L bioreactor. Based on two-dimensional (2D) nuclear magnetic ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10915

    authors: Drysch A,El Massaoudi M,Wiechert W,de Graaf AA,Takors R

    更新日期:2004-03-05 00:00:00