Abstract:
:Continuous cellulase production by Trichoderma viride QM 9123, immobilized in 6 mm diameter, spherical, stainless steel biomass support particles, has been achieved using a medium containing glucose as the main carbon source. Experiments were carried out in a 10-L spouted bed fermentor. In this type of reactor-recycled broth is used to create a jet at the base of a bed of particles, causing the particles to spout and circulate. During the circulation, particles pass through a region of high shear near the jet inlet. This effectively prevents a buildup of excess biomass and thus enables steady-state conditions to be achieved during continuous operation. Continuous production of cellulase was achieved at significantly higher yield and productivity than in conventional systems. At a dilution rate of 0.15 h(-1) (nominal washout rate for freely suspended cells is 0.012 h(-1)), the yield of cellulase on glucose was 31% higher than that measured during batch operation, while the volumetric productivity (31.5 FPA U/L. h) was 53% greater than in the batch system. The specific cellulase productivity of the immobilized cells was more than 3 times that of freely suspended cells, showing that diffusional limitations can be beneficial. This offers significant opportunity for the further development of biomass support particles and associated bioreactors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Webb C,Fukuda H,Atkinson Bdoi
10.1002/bit.260280107subject
Has Abstractpub_date
1986-01-01 00:00:00pages
41-50issue
1eissn
0006-3592issn
1097-0290journal_volume
28pub_type
杂志文章abstract::Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20853
更新日期:2006-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10184
更新日期:2002-05-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2002-03-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400704
更新日期:1992-10-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1002/bit.10428
更新日期:2002-12-30 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250219
更新日期:1983-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22354
更新日期:2009-08-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1998-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1998-04-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/bit.23106
更新日期:2011-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260170905
更新日期:1975-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1994-08-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330706
更新日期:1989-02-20 00:00:00
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pub_type: 杂志文章
doi:10.1002/bit.22119
更新日期:2009-02-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21535
更新日期:2008-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-05-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290213
更新日期:1987-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26976
更新日期:2019-07-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1988-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
abstract::Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390312
更新日期:1992-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251113
更新日期:1983-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390103
更新日期:1992-01-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2008-04-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1998-08-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10915
更新日期:2004-03-05 00:00:00