Abstract：
:Inflammation probably plays a significant role in perinatal brain injury. To study the contribution of locally produced cytokines, the effect on cell death of addition of IL-8 and MCP-1 or antibodies to these, and the impact of acidosis, human postmitotic NT2-N neurons were exposed to 3 h of hypoxia and glucose deprivation and reoxygenated for 21 h. After 3 h of hypoxia with neutral medium, IL-8 was significantly increased compared to controls (150 (100-250)% vs. 100 (85-115)%, p=0.023). After 21 h of neutral reoxygenation, both IL-8 (380 (110-710)% vs. 150 (85-260)%, p=0.041) and monocyte chemoattractant protein-1 (MCP-1) (650 (440-2000)% vs. 310 (230-340)%, p=0.007) were significantly increased compared to controls. After 3 h of hypoxia, both IL-8 (p=0.002) and MCP-1 (p=0.008) were significantly lower in cells with acidotic compared with cells with neutral medium. Acidosis during reoxygenation, however, significantly increased IL-8 release, whereas MCP-1 release was diminished. Similar effects of acidosis were seen in normoxic controls. The cells also secreted RANTES and IP-10, but not 8 other cytokines tested. We found no effect on cell death, measured by MTT assay, of addition of IL-8, MCP-1 or antibodies to these. We conclude that human NT2-N neurons release IL-8 and MCP-1 during 21 h of reoxygenation after 3 h of hypoxia. Acidosis led to a differential effect on IL-8 and MCP-1, with increased IL-8 and decreased MCP-1, both during reoxygenation and in normoxic controls. IL-8 and MCP-1 had no effect on cell death.
journal_name
Brain Resjournal_title
Brain researchauthors
Frøyland E,Pedersen ED,Kvissel AK,Almaas R,Pharo A,Skålhegg BS,Mollnes TE,Rootwelt Tdoi
10.1016/j.brainres.2006.07.051subject
Has Abstractpub_date
2006-10-03 00:00:00pages
64-73issue
1eissn
0006-8993issn
1872-6240pii
S0006-8993(06)02124-Xjournal_volume
1113pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:In recent years, we have carried out several studies on brainstem mechanisms associated with pain modulation. Of particular interest has been the region of the nucleus tractus solitarius (NTS) at the level of the area postrema (AP) in the dorsomedial medulla. This region has been found to be rich in opiate receptors a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91362-8
更新日期：1983-07-18 00:00:00
abstract：:Excessive stimulation of neurons by glutamic acid initiates a destructive cascade of ion fluxes, cellular swelling, and death. Homeostatic mechanisms which rectify these disturbances depend largely upon transmembrane ion gradients maintained by Na+,K(+)-ATPase (NaP). We proposed that the neurotoxicity of glutamate is ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90982-f
更新日期：1992-09-18 00:00:00
abstract：:Estrogen effects on tyrosine hydroxylase (TH), monoamine oxidase types A and B (MAO), and dopamine (DA) in microdissected regions of the hypothalamus, preoptic area and substantia nigra (SNR) of the female rat brain were investigated. Ovariectomized (OVX) young adult female rats were implanted with single silastic cap...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90730-y
更新日期：1990-02-26 00:00:00
abstract：:We examined the effects of nicotinic drugs on the metabolites of [3H]dopamine (DA) released from rat striatal synaptosomes in comparison with the effects of high K+ and methamphetamine stimulation. The pattern of outflow of [3H]DA and its metabolites induced by nicotine stimulants, acetylcholine (ACh), nicotine and lo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90204-4
更新日期：1983-11-21 00:00:00
abstract：:Large neurons in the neostriatum of patients with Alzheimer's disease (AD) and progressive supranuclear palsy (PSP) were investigated topographically, histologically and ultrastructurally. The number of large neurons whose nuclear area is greater than 101 microns2 was uniformly decreased in the neostriatum in PSP, but...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90057-3
更新日期：1991-03-29 00:00:00
abstract：:Axotomy of cholinergic neurons in the medial septum-diagonal band and degeneration of their terminals in the hippocampus resulting from fornix-fimbria lesions induce elevation of NGF content in these two brain regions. Postlesion levels of cholinergic neuron-specific ChAT activity in the septum suggest that endogenous...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90198-8
更新日期：1986-06-25 00:00:00
abstract：:Previous research has suggested the potential involvement of the hypothalamic-pituitary-adrenal (HPA) axis in psychostimulant reinforcement. In particular, we have found significant correlations between electric footshock-induced increases in plasma corticosterone and the acquisition, or lack thereof, of intravenous c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00206-5
更新日期：1996-05-25 00:00:00
abstract：:Long-term potentiation (LTP) consists of an enhanced response to released transmitter by the quisqualate/AMPA subclass of glutamate receptors with little change in the slower currents generated by the NMDA receptor subclass. Recent computer simulations suggest that a decrease in the resistance of dendritic spines woul...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90454-4
更新日期：1991-01-11 00:00:00
abstract：:Previous studies have reported that high concentrations of homocysteine and lower concentrations of vitamins B6, B12, and folate increase the risk for cognitive decline and pathology in aging populations. In this cross-sectional study, high resolution magnetic resonance imaging (MRI) scans and a 3-day food diary were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.01.030
更新日期：2008-03-14 00:00:00
abstract：:The neuroprotective effect of cilostazol, an antiplatelet drug, was examined after 24 h permanent middle cerebral artery (MCA) occlusion in mice, and explored the possible underlying mechanism by examining metallothionein (MT)-1 and -2 induction in vivo. Cilostazol (30 mg/kg) was intraperitoneally administered at 12 h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.07.125
更新日期：2006-10-20 00:00:00
abstract：:Recent studies have demonstrated that gonadectomy of adult male rats induces dendritic growth of neuroendocrine neurons in the arcuate nucleus. We have hypothesized that these changes are secondary to the loss of testosterone negative feedback. In the present study, we examined the effects of testosterone replacement ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03083-3
更新日期：2001-01-26 00:00:00
abstract：:The effects of propylthiouracil (PTU)-induced thyroid deficiency on [14C]acetylcholine synthesis and 14CO2 production from [U-14C]glucose in vitro, by fine prisms of the corpus striatum were investigated in developing rats. Consistent with deficits in choline uptake and choline acetyltransferase activity (Kalaria et a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(85)90114-2
更新日期：1985-06-01 00:00:00
abstract：:To assess the role of androgen-dependent brain vasopressinergic transmission in the modulation of social recognition, castrated male rats which are deficient in vasopressin were implanted intracerebroventricularly with an Accurel collodion mini-device containing 10 micrograms vasopressin (AVP) whereas intact male rats...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90480-w
更新日期：1992-02-14 00:00:00
abstract：:Rats were anesthetized with a continuous infusion of pentobarbital. First we showed that electroacupuncture (EA) suppressed the tail flick nociceptive reflex. Naltrexone (a long acting opiate antagonist) was given either intravenously or intrathecally to attempt to block this EA effect. The naltrexone prevented this E...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90027-3
更新日期：1988-06-14 00:00:00
abstract：:The neurotoxic effects produced by ibotenic acid (IA) induced chemical lesions of the central nervous system (CNS) cholinergic system were examined on the opioid peptidergic system in adult rats. Forebrain cholinergic systems were bilaterally lesioned by the infusion of IA (1 or 5 micrograms/site) into the nucleus bas...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90668-y
更新日期：1992-02-07 00:00:00
abstract：:Neurotrophins play a crucial role in the regulation of survival and the maintenance of specific functions for various populations of neurons. Neurotrophin-4 (NT-4) is most abundant in skeletal muscle, and is thought to promote sciatic nerve sprouting, inhibit agrin-induced acetylcholine receptor (AChR) clustering, evo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02288-0
更新日期：2001-07-13 00:00:00
abstract：:The effects of retinoic acid (RA), a naturally occurring metabolite of vitamin A, on the growth, morphology and neurochemical differentiation of the PC12 clone of rat pheochromocytoma cells were investigated. RA added to the medium inhibited the growth of PC12 cells in a dose-dependent manner up to 10 microM without a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90460-5
更新日期：1989-11-13 00:00:00
abstract：:Available evidence suggests that regular, moderate-intensity exercise has beneficial effects on neural health, perhaps including neuroprotection. To evaluate this idea further, we compared the severity of kainate-induced neuronal loss in exercised versus sedentary female rats. Stereological estimations of neuron numbe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02365-5
更新日期：2003-05-09 00:00:00
abstract：:In chloralose-urethane anaesthetized cats and in one unanesthetized decerebrate cat, the possibility of a baroreceptor pathway exerting an inhibitory effect directly on the sympathetic preganglionic neurone (SPN) was examined. Stimulation of excitatory points in the brain stem and spinal cord evoked responses in T3 wh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90212-2
更新日期：1981-09-07 00:00:00
abstract：:Wild red foxes, proverbially cunning carnivores, are investigated for adult hippocampal neurogenesis and morphological characteristics of the dentate gyrus. Adult red foxes harbor almost 15-times more young, doublecortin-positive neurons in their dentate gyrus than domesticated dogs. The number of doublecortin-positiv...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.02.075
更新日期：2010-04-30 00:00:00
abstract：:We sought to determine whether the global increase in regional cerebral blood flow (rCBF) produced by electrical stimulation of the rostral cerebellar fastigial nucleus (FN) is a consequence of excitation of intrinsic neurons of the FN or of axons of fibers passing through or projecting into it. Studies were conducted...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90312-0
更新日期：1989-10-23 00:00:00
abstract：:We report here that following partial spinal transections in adult lampreys, the fibers of the spinal cords can regenerate and restore some intersegmental coordination to the central pattern generator for locomotion, as tested in the isolated cord preparation. However, the regeneration by this test is not successful i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91090-1
更新日期：1989-09-04 00:00:00
abstract：:An involvement of serotonin (5-HT) 1A receptors in the etiology of psychiatric disorders has been suggested. Hypo-responsiveness of the 5-HT1A receptor is linked to anxiety and constitutive deletion of the 5-HT1A receptor produces anxiety-like behaviors in the mouse. Evidence that 5-HT1A receptor inactivation increase...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02271-5
更新日期：2001-05-25 00:00:00
abstract：:We have examined the question of whether alpha-tubulin is detyrosylated during axonal transport in retinal ganglion cell axons and axons of spinal motor neurons. The degree of tyrosylation of alpha-tubulin was estimated from immunocytochemistry and immunoblotting with two anti-alpha-tubulin monoclonals, one of which (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90697-9
更新日期：1986-08-27 00:00:00
abstract：:The ultrastructural localizations of rat corticoliberin (rCRF) and methionine-enkephalin octapeptide (Met-Enk-8) in the external layer of the rat median eminence were examined by double-immunogold labeling with anti-rCRF and anti-Met-Enk-8 sera labeled with small-sized (Gs) and medium-sized (Gm) gold particles, respec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90487-7
更新日期：1986-04-09 00:00:00
abstract：:The excitability of the axonal terminals of medial septal neurons projecting to the dentate gyrus has been studied in the anesthetized rat under various experimental conditions: spontaneous or drug-induced variations in neuronal soma discharge rate, conditioning stimulation of afferent pathways (perforant path, commis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90966-8
更新日期：1987-08-18 00:00:00
abstract：:After treatment with pentylenetetrazol (PTZ), cholecystokinin (CCK) mRNA and CCK-like immunoreactivity (CCK-LI) levels were determined in rat hippocampus and cortex at different time points. In the temporal cortex treatment with 60 mg/kg PTZ, i.p., induced increases of CCK mRNA and CCK-LI levels at 2 days after the in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01179-7
更新日期：1998-01-01 00:00:00
abstract：:The monoclonal antibody E/C8 was originally generated against chicken dorsal root ganglia. The antigen detected by E/C8, NAPA-73, is an intermediate filament-associated protein. NAPA-73 was shown to be one of the earliest neuronal markers to appear during development since it appears in some neuronal precursor cells o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91289-3
更新日期：1987-09-22 00:00:00
abstract：:Despite a blood-brain barrier for norepinephrine, the concentration of norepinephrine in plasma and cerebrospinal fluid has been observed to be similar. This relationship between plasma and cerebrospinal fluid norepinephrine levels suggest that peripheral sympathetic neurons innervating blood vessels to brain and spin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91600-8
更新日期：1986-03-05 00:00:00
abstract：:Two different ligands, tritiated oxytocin and a newly synthesized and monoiodinated oxytocin antagonist, were used to reveal sites which bind oxytocin in the brainstem and upper spinal cord of 12 human subjects. Tissue sections were incubated with either ligand at a concentration close to their respective dissociation...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90317-x
更新日期：1989-10-23 00:00:00
