Abstract:
:In the last 10 years, saliva has been increasingly used as a diagnostic fluid and in predictions of disease progression. Leptin and ghrelin are synthesized in several tissues including the salivary glands. The action of ghrelin is antagonistic to that of leptin. This study was undertaken to measure and compare the saliva ghrelin-leptin and plasma ghrelin-leptin levels in healthy young subjects. In 30 healthy subjects, after an overnight fast, saliva and plasma leptin levels were measured using the ELISA method while saliva and plasma immunoreactive ghrelin levels were measured using a commercial radioimmunoassay (RIA). The latter uses 125I-labeled bioactive ghrelin as a tracer and a rabbit polyclonal antibody raised against full-length octanoylated human ghrelin (Phoenix, Europe, Karlsruhe, Germany). The results of this investigation revealed that saliva leptin levels (6.19+/-2.10 microg/l) were lower than plasma levels (7.39+/-3.23 microg/l) while saliva ghrelin levels (188.5+/-84.7 pg/ml) were higher than plasma levels (126.4+/-38.5 pg/ml), when male and female subjects were considered together. Saliva leptin levels (5.93+/-1.94 microg/l) were lower than plasma levels (6.22+/-2.92 pg/ml) while saliva ghrelin levels (190.3+/-80.2 pg/ml) were higher than plasma levels (120.4+/-35.7 pg/ml) in young males. Saliva leptin levels (6.47+/-2.29 microg/l) were lower than plasma levels (8.73+/-3.14 microg/l) while saliva ghrelin levels (183.2+/-90.2 pg/ml) were higher than plasma levels (129.3+/-42.8 pg/ml) in young females, and both saliva and plasma leptin levels were slightly lower in male subjects in comparison with female subjects. Also, Immunohistochemistry study indicated that ghrelin positivity was found in ductus epithelium of salivary gland. We have demonstrated for the first time that saliva ghrelin levels were higher than in plasma while saliva leptin levels were almost the same as in plasma. Measurements of ghrelin and leptin in saliva is non-invasive, simple, and generally much preferred by patients and thus may be an acceptable alternative to plasma sampling.
journal_name
Peptidesjournal_title
Peptidesauthors
Aydin S,Halifeoglu I,Ozercan IH,Erman F,Kilic N,Aydin S,Ilhan N,Ilhan N,Ozkan Y,Akpolat N,Sert L,Caylak Edoi
10.1016/j.peptides.2004.11.008subject
Has Abstractpub_date
2005-04-01 00:00:00pages
647-52issue
4eissn
0196-9781issn
1873-5169pii
S0196-9781(04)00507-8journal_volume
26pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Nesfatin-1 is well established to reduce food intake upon brain injection in rats, while in mice its anorexigenic action and brain expression are largely unexplored. We characterized the influence of intracerebroventricular (icv) and peripheral (intraperitoneal, ip, subcutaneous, sc) injection of nesfatin-1 on dark ph...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.09.027
更新日期:2011-01-01 00:00:00
abstract::The effects of arginine vasotocin (AVT), 4-6 ng/kg, on renal function and plasma prolactin (PRL) were determined in conscious American foxhounds undergoing a water diuresis. Intravenous AVT produced an antidiuresis, kaliuresis and inconsistent natriuresis, independently of changes in GFR, blood pressure, heart rate an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90062-6
更新日期:1982-11-01 00:00:00
abstract::Kisspeptins, a family of peptide products derived from the KiSS-1 gene, activate their cognate receptor GPR54 in various target tissues to exert disparate functions, including inhibition of tumor metastasis and control of reproductive function. In contrast to the plethora of studies that have analyzed in recent years ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2008.07.025
更新日期:2009-01-01 00:00:00
abstract::GIP receptor knockout mice were shown to be protected from the development of obesity on a high fat diet, suggesting a role of GIP in the development of obesity. In our study we aimed to test the hypothesis if excess of GIP could accelerate development of obesity and to identify GIP gene targets in adipose tissue. The...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.02.011
更新日期:2011-05-01 00:00:00
abstract::Trefoil factors family 2 (TFF2), also known as spasmolytic polypeptide, is primarily expressed in the mucus neck cells of gastrointestinal tracts. It has been proposed that TFF2 plays an important physiological role in protection, repair, and healing of gastrointestinal mucosa. To investigate the cis-acting regulatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.11.022
更新日期:2004-05-01 00:00:00
abstract::Plant defensins are a prominent family of cationic peptides in the plant kingdom. They are structurally and functionally related to defensins that have been previously characterized in mammals and insects. They present molecular masses between 5 and 7kDa and possess a pattern of eight conserved Cys residues. The three...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2009.01.018
更新日期:2009-05-01 00:00:00
abstract::Malaria lactate dehydrogenase, a glycolytic enzyme, is a malaria diagnostic target in lateral flow immunochromatographic rapid diagnostic tests. Recombinant Plasmodium yoelii LDH was cloned into the pET-28a vector, expressed and the expressed protein purified from a Ni-NTA affinity matrix. A pan-malarial LDH antibody ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.01.002
更新日期:2010-04-01 00:00:00
abstract::Apelin, a newly identified bioactive adipokine, has been found to play important roles in multiple diseases, including diabetes, hypertension and cardiovascular diseases with unclear molecular mechanisms. The present study aimed to investigate the effects of apelin on endoplasmic reticulum (ER) stress in the pancreas ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.06.025
更新日期:2011-08-01 00:00:00
abstract::LL-37 is the single cathelicidin host defense peptide in humans with direct antimicrobial and immunomodulatory activities. Specific regulation of LL-37 synthesis has emerged as a novel non-antibiotic approach to disease control and prevention. Short-chain fatty acids, and butyrate in particular, were found recently to...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.10.008
更新日期:2013-12-01 00:00:00
abstract::Venoms from cone snails (genus Conus) can be seen as an untapped cocktail of biologically active compounds, being increasingly recognized as an emerging source of peptide-based therapeutics. Cone snails are considered to be specialized predators that have evolved the most sophisticated peptide chemistry and neuropharm...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.07.002
更新日期:2013-03-01 00:00:00
abstract::Nervous, endocrine, and immune systems share a large number of regulatory molecules including hormones and neuropeptides. Vasoactive intestinal peptide (VIP) plays an important role in a variety of immunological functions. In the present report, we sorted purified thymocytes of the four major thymic subsets defined by...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00070-8
更新日期:1996-01-01 00:00:00
abstract::Humanin (HN) and its analogues have been shown to protect cells against death induced by various Alzheimer's disease (AD) genes and amyloid-beta-peptides in vitro; the analogues [Gly(14)]-HN and colivelin have also been shown to be potent in reversing learning and memory impairment induced by scopolamine or quinuclidi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.06.019
更新日期:2008-11-01 00:00:00
abstract::Rhesus monkey MCH-R1 and MCH-R2 receptors were cloned. Amino acid homology is 98.8% between monkey and human MCH-R1, while monkey and human MCH-R2 are 98% homologous. Binding and intracellular signaling characteristics of the monkey receptors were compared with the human homologues. The results demonstrate that MCH bi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00077-3
更新日期:2002-08-01 00:00:00
abstract::The neuropeptide Y system is known to have expanded in early vertebrate evolution. Three neuropeptide Y receptors have been proposed to have existed before the two basal vertebrate tetraploidizations, namely a Y1-like, a Y2-like, and a Y5-like receptor, with their genes in the same chromosomal region. Previously we ha...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.11.007
更新日期:2013-01-01 00:00:00
abstract::Receptors for neuropeptide Y (NPY) and peptide YY (PYY) have been extensively characterized in the brain. Less is known about NPY receptor subtypes in the spleen, though it is well established that NPY produces vascular contraction in this tissue. In the present study, we found an unusually high density of Y1 receptor...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(96)00228-8
更新日期:1996-01-01 00:00:00
abstract::Venom small peptides that target neurotrophin receptors might be beneficial in neurodegeneration, including Parkinsońs disease (PD). Their small size, ease of synthesis, structural stability and target selectivity make them important tools to overcome the limitations of endogenous neurotrophins as therapeutic agents. ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.04.012
更新日期:2018-06-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP) is a neuropeptide present in high concentrations in the hippocampus. The studies reported here demonstrate that VIP administered into the third ventricle of the brain caused amnesia in mice trained on a left-right footshock avoidance task in a T-maze. VIP resulted in amnesia when ad...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90012-t
更新日期:1990-09-01 00:00:00
abstract::Both diapause hormone (DH) and ecdysone (E) are capable of terminating pupal diapause in members of the Helicoverpa/Heliothis complex. In this study we examine how the transcript encoding prothoracicotropic hormone (PTTH), the neuropeptide that stimulates the prothoracic gland to produce E, and the transcript encoding...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.05.019
更新日期:2012-03-01 00:00:00
abstract::Cone snails comprise approximately 700 species of venomous molluscs which have evolved the ability to generate multiple toxins with varied and exquisite selectivity. alpha-Conotoxin is a powerful tool for defining the composition and function of nicotinic acetylcholine receptors which play a crucial role in excitatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.05.028
更新日期:2008-10-01 00:00:00
abstract::The amino acid sequence of the vasoactive intestinal polypeptide (VIP) is well conserved between species. Thus, all mammalian VIPs isolated so far, except that of the guinea pig, have the same amino acid sequence. This study describes the isolation and primary structure of sheep brain VIP. The purification was followe...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90184-7
更新日期:1990-07-01 00:00:00
abstract::Gastrin/CCK peptides in extracts from bovine and porcine pituitary have been characterized by Sephadex gel filtration, reverse phase liquid chromatography and a CCK radioimmunoassay (RIA) which detects both CCK and gastrin [4]. Porcine pituitary extracts contain a small amount of two peptides with chromatographic beha...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90121-8
更新日期:1982-05-01 00:00:00
abstract::Members of the neprilysin family of neutral endopeptidases (M13) are typically membrane-bound enzymes known to be involved in the extra-cellular metabolism of signalling peptides and have important roles during mammalian embryogenesis. In this study we show that membranes prepared from embryos of Drosophila melanogast...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.032
更新日期:2007-01-01 00:00:00
abstract::Japonicin-1 (FFPIGVFCKIFKTC) and japonicin-2 (FGLPMLSILPKALCILLKRKC), two peptides with differential growth-inhibitory activity against the Gram-negative bacterium, Escherichia coli and the Gram-positive bacterium Staphylococcus aureus, were isolated from an extract of the skin of the Japanese brown frog Rana japonica...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00634-9
更新日期:2002-03-01 00:00:00
abstract::The purpose of this study was to investigate in the aged animal the functional interaction between cholecystokinin (CCK) and amino acid neurotransmitter activity in the frontal cortex, a structure of importance in age-related disabilities. Guide cannula for repeated push-pull perfusion were implanted bilaterally in th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90175-1
更新日期:1988-05-01 00:00:00
abstract::Central nervous systems of the pond snail Lymnaea stagnalis were incubated in vitro in different concentrations of ORG 2766 (10(-9)-2.5 x 10(-4) M) for 10 and 20 h. Quantitative ultrastructural study of cross sections of the cerebral commissure showed that the number of microtubules in large axons had increased after ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90185-6
更新日期:1992-07-01 00:00:00
abstract::An important question in contemporary sensory neuroscience is how animals perceive their environment and make appropriate behavioral choices based on chemical perceptions. The fruit fly Drosophila melanogaster exhibits robust tastant and odor-evoked behaviors. Understanding how the gustatory and olfactory systems supp...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2012.09.019
更新日期:2012-12-01 00:00:00
abstract::Intracerebroventricular administration of NPY suppresses ethanol intake in selectively bred alcohol-preferring rat lines, but not in rats selectively bred for low ethanol drinking or in unselected Wistar rats, when access to ethanol is limited to 2h/day. However, when rats undergo chronic (24h/day) ethanol drinking (o...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2006.07.028
更新日期:2007-02-01 00:00:00
abstract::The presence of alpha-hANP immunoreactive material in human heart and plasma was investigated with a specific and sensitive radioimmunoassay and immunohistochemical method. It was found that alpha-hANP immunoreactive staining of specific atrial granules was located around the nucleus of atrial cardiocytes. No immunore...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90057-4
更新日期:1986-01-01 00:00:00
abstract::Sex pheromone biosynthesis in many moth species is controlled by a cerebral neuropeptide, termed pheromone biosynthesis activating neuropeptide (PBAN). PBAN is a 33 amino acid C-terminally amidated neuropeptide that is produced by neuroendocrine cells of the subesophageal ganglion (SEG). Studies of the regulation of s...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2004.06.020
更新日期:2004-09-01 00:00:00
abstract::Many neuroendocrine peptides that are distributed in amphibian gastrointestinal tract and central nervous system are also found in amphibian skins, and these peptides are classified into skin-gut-brain triangle peptides, such as bombesins, gastrin-releasing peptides. Cholecystokinins (CCKs) are neuroendocrine peptides...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.07.006
更新日期:2007-08-01 00:00:00