Abstract:
:The time course and functional significance of the structural changes associated with long-term facilitation of Aplysia sensory to motor neuron synaptic connections in culture were examined by time-lapse confocal imaging of individual sensory neuron varicosities labeled with three different fluorescent markers: the whole-cell marker Alexa-594 and two presynaptic marker proteins-synaptophysin-eGFP to monitor changes in synaptic vesicle distribution and synapto-PHluorin to monitor active transmitter release sites. Repeated pulses of serotonin induce two temporally, morphologically, and molecularly distinct presynaptic changes: (1) a rapid activation of silent presynaptic terminals by filling of preexisting empty varicosities with synaptic vesicles, which parallels intermediate-term facilitation, is completed within 3-6 hr and requires translation but not transcription and (2) a slower generation of new functional varicosities which occurs between 12-18 hr and requires transcription and translation. Enrichment of empty varicosities with synaptophysin accounts for 32% of the newly activated synapses at 24 hr, whereas newly formed varicosities account for 68%.
journal_name
Neuronjournal_title
Neuronauthors
Kim JH,Udo H,Li HL,Youn TY,Chen M,Kandel ER,Bailey CHdoi
10.1016/s0896-6273(03)00595-6subject
Has Abstractpub_date
2003-09-25 00:00:00pages
151-65issue
1eissn
0896-6273issn
1097-4199pii
S0896627303005956journal_volume
40pub_type
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