Abstract:
:The Fragile X mental retardation-1 (Fmr1) gene encodes a multifunctional protein, FMRP, with intrinsic RNA binding activity. We have developed an approach, antibody-positioned RNA amplification (APRA), to identify the RNA cargoes associated with the in vivo configured FMRP messenger ribonucleoprotein (mRNP) complex. Using APRA as a primary screen, putative FMRP RNA cargoes were assayed for their ability to bind directly to FMRP using traditional methods of assessing RNA-protein interactions, including UV-crosslinking and filter binding assays. Approximately 60% of the APRA-defined mRNAs directly associate with FMRP. By examining a subset of these mRNAs and their encoded proteins in brain tissue from Fmr1 knockout mice, we have observed that some of these cargoes as well as the proteins they encode show discrete changes in abundance and/or differential subcellular distribution. These data are consistent with spatially selective regulation of multiple biological pathways by FMRP.
journal_name
Neuronjournal_title
Neuronauthors
Miyashiro KY,Beckel-Mitchener A,Purk TP,Becker KG,Barret T,Liu L,Carbonetto S,Weiler IJ,Greenough WT,Eberwine Jdoi
10.1016/s0896-6273(03)00034-5subject
Has Abstractpub_date
2003-02-06 00:00:00pages
417-31issue
3eissn
0896-6273issn
1097-4199pii
S0896627303000345journal_volume
37pub_type
杂志文章相关文献
NEURON文献大全abstract::Currents mediated by a glutamate transporter cloned from human motor cortex were measured in Xenopus oocytes. In the absence of glutamate, voltage jumps induced Na(+)-dependent capacitive currents that were blocked by kainate, a competitive transport antagonist. The pre-steady-state currents can be described by an ord...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(95)90340-2
更新日期:1995-05-01 00:00:00
abstract::Protrusive behavior of dendritic spines on developing neurons has been previously suggested to mediate the formation of new axodendritic synaptic contacts. A study by Zito et al. in this issue of Neuron links actin polymerization in dendritic spines with the motility that the spines exhibit and the synapses that they ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2004.10.003
更新日期:2004-10-14 00:00:00
abstract::Neuromuscular junction (NMJ) formation requires agrin, a factor released from motoneurons, and MuSK, a transmembrane tyrosine kinase that is activated by agrin. However, how signal is transduced from agrin to MuSK remains unclear. We report that LRP4, a low-density lipoprotein receptor (LDLR)-related protein, is expre...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.10.006
更新日期:2008-10-23 00:00:00
abstract::In this issue of Neuron, Oh and Gu (2013) present a model in which intimately related embryonic nerves and blood vessels are patterned independently in response to different guidance cues from a central organizer: the whisker. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2013.09.040
更新日期:2013-10-16 00:00:00
abstract::Brandon et al. (2014) show that the formation of place cell representations in new environments is preserved under septal inactivation, and is thus likely independent of the hippocampal theta rhythm and, by implication, the firing of entorhinal grid cells and the process of path integration. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2014.05.011
更新日期:2014-05-21 00:00:00
abstract::Functional neuroimaging uses activity-dependent changes in cerebral blood flow to map brain activity, but the contributions of presynaptic and postsynaptic activity are incompletely understood, as are the underlying cellular pathways. Using intravital multiphoton microscopy, we measured presynaptic activity, postsynap...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.04.029
更新日期:2008-06-26 00:00:00
abstract::Retinal specializations such as cone-photoreceptor opsin-expression gradients, as found in several vertebrate species, are intuitively considered detrimental to color vision. In mice, the majority of cones coexpress both "blue" and "green" opsin. The coexpression ratio changes along the dorsoventral axis, resulting in...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.12.012
更新日期:2013-02-06 00:00:00
abstract::Protein kinase A (PKA) is a key regulator of neurosecretion, but the molecular targets remain elusive. We combined pharmacological manipulations of kinase and phosphatase activities with mutational studies on the exocytotic machinery driving fusion of catecholamine-containing vesicles from chromaffin cells. We found t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(04)00038-8
更新日期:2004-02-05 00:00:00
abstract::The brain basis of language, music, and emotion can be studied from the perspective of the psychological and cognitive sciences. Does this approach link to concerns of the humanities meaningfully? We outline prospects of developing a genuine neurohumanities research program. ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2020.10.021
更新日期:2020-11-25 00:00:00
abstract::Genetic deletion of fragile X mental retardation protein (FMRP) has been shown to enhance mGluR-dependent long-term depression (LTD). Herein, we demonstrate that mGluR-LTD induces a transient, translation-dependent increase in FMRP that is rapidly degraded by the ubiquitin-proteasome pathway. Moreover, proteasome inhi...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.07.005
更新日期:2006-08-17 00:00:00
abstract::In this issue of Neuron, Ambrozkiewicz et al. (2018) define a new molecular circuit controlling neuronal polarization and migration through the transcription factor SOX9 to coordinate the production of regulators of both protein synthesis and degradation. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2018.11.022
更新日期:2018-12-05 00:00:00
abstract::Previous research suggests that synchronous neural activity underlies perceptual grouping of visual image features. The generality of this mechanism is unclear, however, as previous studies have focused on pairs of neurons with overlapping or collinear receptive fields. By sampling more broadly and employing stimuli t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2005.03.002
更新日期:2005-04-21 00:00:00
abstract::Postlingually deaf subjects learn the meaning of sounds after cochlear implantation by forming new associations between sounds and their sources. Implants generate coarse frequency responses, preventing place-coding fine enough to discriminate sounds with similar temporal characteristics, e.g., buck/duck. This limitat...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(01)00318-x
更新日期:2001-06-01 00:00:00
abstract::Today we are witnessing a new science of placebo, a complex discipline that encompasses several experimental approaches and translational implications. Modern neurobiological tools have been used to answer important questions in placebo research, such as the top-down modulation of sensory and motor systems as well as ...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2014.10.023
更新日期:2014-11-05 00:00:00
abstract::Mammalian neurogenesis is determined by an interplay between intrinsic genetic mechanisms and extrinsic cues such as growth factors. Here we have defined a signaling cascade, a MEK-C/EBP pathway, that is essential for cortical progenitor cells to become postmitotic neurons. Inhibition of MEK or of the C/EBP family of ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)01026-7
更新日期:2002-11-14 00:00:00
abstract::Recent studies indicate that long interspersed nuclear element-1 (L1) are mobilized in the genome of human neural progenitor cells and enhanced in Rett syndrome and ataxia telangiectasia. However, whether aberrant L1 retrotransposition occurs in mental disorders is unknown. Here, we report high L1 copy number in schiz...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.10.053
更新日期:2014-01-22 00:00:00
abstract::Two types of ryanodine receptors, channels for Ca2+ release from intracellular stores, are known. We detected the skeletal muscle type only in cerebellum by immunoblot analysis of microsomes and partially purified proteins. The cardiac muscle type was found in all parts of the mouse brain. Immunohistochemical study sh...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90071-k
更新日期:1992-12-01 00:00:00
abstract::Memory enables flexible use of past experience to inform new behaviors. Although leading theories hypothesize that this fundamental flexibility results from the formation of integrated memory networks relating multiple experiences, the neural mechanisms that support memory integration are not well understood. Here, we...
journal_title:Neuron
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.neuron.2012.05.010
更新日期:2012-07-12 00:00:00
abstract::During the metamorphosis of the Xenopus laevis retina, thyroid hormone (TH) preferentially induces ventral ciliary marginal zone (CMZ) cells to both increase their proliferation and give rise to ipsilaterally projecting ganglion cells. Here we show that dorsal CMZ cells express type III deiodinase (D3), an enzyme that...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)81034-x
更新日期:1999-12-01 00:00:00
abstract::In daylight, the input to the retinal circuit is provided primarily by cone photoreceptors acting as band-pass filters, but the retinal output also contains neuronal populations transmitting sustained signals. Using in vivo imaging of genetically encoded calcium reporters, we investigated the circuits that generate th...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2016.03.015
更新日期:2016-04-20 00:00:00
abstract::The replay of previously stored memories during hippocampal sharp wave ripples (SWRs) is thought to support both memory retrieval and consolidation in distributed hippocampal-neocortical circuits. Replay events consist of precisely timed sequences of spikes from CA3 and CA1 neurons that are coordinated both within and...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.06.014
更新日期:2012-08-23 00:00:00
abstract::Critical periods are developmental time windows during which neuronal connections are shaped by experience. In this issue of Neuron, Davis et al. (2015) show that transplantation of embryonic inhibitory interneurons can reactivate critical period plasticity and reverse amblyopia in the visual cortex of adult mice. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2015.05.003
更新日期:2015-05-20 00:00:00
abstract::The biologically active photoproduct of rhodopsin, metarhodopsin II (M II), exists in a pH-sensitive equilibrium with its precursor, metarhodopsin I (M I). Increasing acidity favors M II, with the midpoint of the pH titration curve at pH 6.4. To test the long-standing proposal that histidine protonation regulates this...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90274-h
更新日期:1992-03-01 00:00:00
abstract::Neuronal motor commands, whether generating real or neuroprosthetic movements, are shaped by ongoing sensory feedback from the displacement being produced. Here we asked if cortical stimulation could provide artificial feedback during operant conditioning of cortical neurons. Simultaneous two-photon imaging and real-t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.01.023
更新日期:2017-02-22 00:00:00
abstract::The posterior septum consisting of the triangular septum (TS) and the bed nucleus of the anterior commissure (BAC) is predominantly linked with the medial habenula (MHb) and has been implicated in the control of anxiety and fear responses. However, its anatomical and functional linkage has largely remained elusive. We...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.02.035
更新日期:2013-05-08 00:00:00
abstract::The role of early visual experience in the establishment of human high-order visual areas is poorly understood. Here we investigated this issue using human amblyopia--a developmental visual disorder, which manifests a central vision (acuity) deficit. Previous fMRI studies of amblyopes have described abnormal functiona...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00720-7
更新日期:2003-12-04 00:00:00
abstract::The histone deacetylase HDAC5 has been shown to regulate behavioral adaptations to cocaine. In this issue of Neuron, Taniguchi et al. (2012) describe a cAMP-dependent signaling pathway that regulates nuclear accumulation of HDAC5, suggesting a mechanism to couple cocaine with changes in HDAC5 function. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2011.12.016
更新日期:2012-01-12 00:00:00
abstract::Astrocytes release glutamate, which can modulate nearby synapses, but how this release is accomplished remains debated. In this issue of Neuron, Yang et al. (2019) show that the volume-regulated anion channel Swell1 in astrocytes mediates the release of glutamate and impacts synaptic transmission, learning and memory,...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2019.05.005
更新日期:2019-05-22 00:00:00
abstract::Striatal dopamine plays key roles in our normal and pathological goal-directed actions. To understand dopamine function, much attention has focused on how midbrain dopamine neurons modulate their firing patterns. However, we identify a presynaptic mechanism that triggers dopamine release directly, bypassing activity i...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.04.038
更新日期:2012-07-12 00:00:00
abstract::Mapping of the chromosomal location of genes essential for sweet and bitter taste and identification of the relevant G protein-coupled receptors reveals unanticipated complexity in taste signaling pathways. The distribution of sweet and bitter receptors suggests complete cellular segregation of these taste modalities....
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/s0896-6273(01)00404-4
更新日期:2001-08-30 00:00:00