Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25.

Abstract:

:Protein kinase A (PKA) is a key regulator of neurosecretion, but the molecular targets remain elusive. We combined pharmacological manipulations of kinase and phosphatase activities with mutational studies on the exocytotic machinery driving fusion of catecholamine-containing vesicles from chromaffin cells. We found that constitutive PKA activity was necessary to maintain a large number of vesicles in the release-ready, so-called primed, state, whereas calcineurin (protein phosphatase 2B) activity antagonized this effect. Overexpression of the SNARE protein SNAP-25a mutated in a PKA phosphorylation site (Thr-138) eliminated the effect of PKA inhibitors on the vesicle priming process. Another, unidentified, PKA target regulated the relative size of two different primed vesicle pools that are distinguished by their release kinetics. Overexpression of the SNAP-25b isoform increased the size of both primed vesicle pools by a factor of two, and mutations in the conserved Thr-138 site had similar effects as in the a isoform.

journal_name

Neuron

journal_title

Neuron

authors

Nagy G,Reim K,Matti U,Brose N,Binz T,Rettig J,Neher E,Sørensen JB

doi

10.1016/s0896-6273(04)00038-8

subject

Has Abstract

pub_date

2004-02-05 00:00:00

pages

417-29

issue

3

eissn

0896-6273

issn

1097-4199

pii

S0896627304000388

journal_volume

41

pub_type

杂志文章

相关文献

NEURON文献大全
  • Temporal and spatial modulation of a cytoskeletal antigen during peripheral axonal pathfinding.

    abstract::A neuron-specific cytoskeletal antigen (5E10), whose expression pattern during initial motoneuron outgrowth into the chick limb suggests that it is playing a role in axon guidance, is described. This antigen, which was shown to be a phosphorylated epitope, probably of the intermediate weight neurofilament protein (NF-...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(92)90296-p

    authors: Landmesser L,Swain S

    更新日期:1992-02-01 00:00:00

  • A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death.

    abstract::Sympathetic neurons depend on nerve growth factor (NGF) for survival and die by apoptosis in its absence. We have investigated the pattern of expression of the Jun and Fos family of transcription factors in dying sympathetic neurons using antibodies specific for each family member. When sympathetic neurons are deprive...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(95)90331-3

    authors: Ham J,Babij C,Whitfield J,Pfarr CM,Lallemand D,Yaniv M,Rubin LL

    更新日期:1995-05-01 00:00:00

  • Testosterone reduction prevents phenotypic expression in a transgenic mouse model of spinal and bulbar muscular atrophy.

    abstract::Spinal and bulbar muscular atrophy (SBMA) is a polyglutamine disease caused by the expansion of a CAG repeat in the androgen receptor (AR) gene. We generated a transgenic mouse model carrying a full-length AR containing 97 CAGs. Three of the five lines showed progressive muscular atrophy and weakness as well as diffus...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(02)00834-6

    authors: Katsuno M,Adachi H,Kume A,Li M,Nakagomi Y,Niwa H,Sang C,Kobayashi Y,Doyu M,Sobue G

    更新日期:2002-08-29 00:00:00

  • A subpopulation of cultured avian neural crest cells has transient neurogenic potential.

    abstract::Neural crest cells of vertebrate embryos produce neurons, glia, pigment cells, and connective tissue in vivo and in vitro. To test the developmental potential of apparently undifferentiated crest cells, we have used the monoclonal antibody A2B5, which recognizes a cell surface glycolipid characteristic of neurons, to ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(88)90106-7

    authors: Vogel KS,Weston JA

    更新日期:1988-09-01 00:00:00

  • Huntingtin-interacting protein HIP14 is a palmitoyl transferase involved in palmitoylation and trafficking of multiple neuronal proteins.

    abstract::In neurons, posttranslational modification by palmitate regulates the trafficking and function of signaling molecules, neurotransmitter receptors, and associated synaptic scaffolding proteins. However, the enzymatic machinery involved in protein palmitoylation has remained elusive. Here, using biochemical assays, we s...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2004.11.027

    authors: Huang K,Yanai A,Kang R,Arstikaitis P,Singaraja RR,Metzler M,Mullard A,Haigh B,Gauthier-Campbell C,Gutekunst CA,Hayden MR,El-Husseini A

    更新日期:2004-12-16 00:00:00

  • mTOR'ing across the Cortex by Chopping the Cilia.

    abstract::Somatic mutation of the MTOR gene is a genetic etiology of focal malformations of cortical development. In this issue of Neuron, Park et al. (2018) identify defective autophagy-dependent ciliogenesis/Wnt signaling as an underlying mechanism affecting neuronal migration and cortical lamination. ...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2018.06.042

    authors: Di Nardo A,Sahin M

    更新日期:2018-07-11 00:00:00

  • Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel.

    abstract::The intracellular C-terminal domain structure of a six-transmembrane K+ channel from Escherichia coli has been solved by X-ray crystallography at 2.4 A resolution. The structure is representative of a broad class of domains/proteins that regulate the conductance of K+ (here referred to as RCK domains) in prokaryotic K...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(01)00236-7

    authors: Jiang Y,Pico A,Cadene M,Chait BT,MacKinnon R

    更新日期:2001-03-01 00:00:00

  • Cellular clocks in AVP neurons of the SCN are critical for interneuronal coupling regulating circadian behavior rhythm.

    abstract::The suprachiasmatic nucleus (SCN), the primary circadian pacemaker in mammals, is a network structure composed of multiple types of neurons. Here, we report that mice with a Bmal1 deletion specific to arginine vasopressin (AVP)-producing neurons showed marked lengthening in the free-running period and activity time of...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2015.02.005

    authors: Mieda M,Ono D,Hasegawa E,Okamoto H,Honma K,Honma S,Sakurai T

    更新日期:2015-03-04 00:00:00

  • Transglutaminase and polyamination of tubulin: posttranslational modification for stabilizing axonal microtubules.

    abstract::Neuronal microtubules support intracellular transport, facilitate axon growth, and form a basis for neuronal morphology. While microtubules in nonneuronal cells are depolymerized by cold, Ca(2+), or antimitotic drugs, neuronal microtubules are unusually stable. Such stability is important for normal axon growth and ma...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2013.01.036

    authors: Song Y,Kirkpatrick LL,Schilling AB,Helseth DL,Chabot N,Keillor JW,Johnson GV,Brady ST

    更新日期:2013-04-10 00:00:00

  • Beyond the Stimulus: A Neurohumanities Approach to Language, Music, and Emotion.

    abstract::The brain basis of language, music, and emotion can be studied from the perspective of the psychological and cognitive sciences. Does this approach link to concerns of the humanities meaningfully? We outline prospects of developing a genuine neurohumanities research program. ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2020.10.021

    authors: Hartley CA,Poeppel D

    更新日期:2020-11-25 00:00:00

  • Seeing is believing: GFP transgenics illuminate synapse elimination.

    abstract::Synapses are lost during developmental synapse elimination. Until now, it has been impossible to follow elimination in the entirety of any neuron's branches and synapses. Using transgenic mice in which one or two of a muscle's motoneurons express variants of GFP, Keller-Peck et al. show in this issue of Neuron that el...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/s0896-6273(01)00380-4

    authors: Thompson WJ

    更新日期:2001-08-16 00:00:00

  • TRPV1 channels are intrinsically heat sensitive and negatively regulated by phosphoinositide lipids.

    abstract::The capsaicin receptor, TRPV1, is regulated by phosphatidylinositol-4,5-bisphosphate (PIP(2)), although the precise nature of this effect (i.e., positive or negative) remains controversial. Here, we reconstitute purified TRPV1 into artificial liposomes, where it is gated robustly by capsaicin, protons, spider toxins, ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2012.12.016

    authors: Cao E,Cordero-Morales JF,Liu B,Qin F,Julius D

    更新日期:2013-02-20 00:00:00

  • Temporal integration of olfactory perceptual evidence in human orbitofrontal cortex.

    abstract::Given a noisy sensory world, the nervous system integrates perceptual evidence over time to optimize decision-making. Neurophysiological accumulation of sensory information is well-documented in the animal visual system, but how such mechanisms are instantiated in the human brain remains poorly understood. Here we com...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2012.06.035

    authors: Bowman NE,Kording KP,Gottfried JA

    更新日期:2012-09-06 00:00:00

  • Dendritic h channelopathy in epileptogenesis.

    abstract::Recent work has suggested a link between h channels and epilepsy. In this issue of Neuron, Shah et al. demonstrate that a robust, postseizure decrease in h channels during a critical phase of epileptogenesis mechanistically underlies dendritic hyperexcitability in entorhinal-hippocampal pyramidal cells. ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2004.10.018

    authors: Dyhrfjeld-Johnsen J,Soltesz I

    更新日期:2004-10-28 00:00:00

  • LRP4 serves as a coreceptor of agrin.

    abstract::Neuromuscular junction (NMJ) formation requires agrin, a factor released from motoneurons, and MuSK, a transmembrane tyrosine kinase that is activated by agrin. However, how signal is transduced from agrin to MuSK remains unclear. We report that LRP4, a low-density lipoprotein receptor (LDLR)-related protein, is expre...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2008.10.006

    authors: Zhang B,Luo S,Wang Q,Suzuki T,Xiong WC,Mei L

    更新日期:2008-10-23 00:00:00

  • Oscillatory substrates of fear and safety.

    abstract::Oscillatory activity in the basolateral amygdala (BLA) is critical for emotional behavior. In this issue of Neuron, Stujenske et al. (2014) describe novel dynamics of BLA theta-gamma-coupled neuronal oscillations associated with conditioned and innate fear. ...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2014.08.014

    authors: Bocchio M,Capogna M

    更新日期:2014-08-20 00:00:00

  • NMDA receptors, glial cells, and clinical medicine.

    abstract::Recent reports have overturned a series of dogmas that have been well entrenched in the neuroscience literature concerning NMDA-type glutamate receptors (NMDARs). The new data show that NMDARs exist on the myelin sheath formed by oligodendrocytes, that an uncompetitive NMDAR antagonist has successfully passed human cl...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2006.03.026

    authors: Lipton SA

    更新日期:2006-04-06 00:00:00

  • A modeling framework for deriving the structural and functional architecture of a short-term memory microcircuit.

    abstract::Although many studies have identified neural correlates of memory, relatively little is known about the circuit properties connecting single-neuron physiology to behavior. Here we developed a modeling framework to bridge this gap and identify circuit interactions capable of maintaining short-term memory. Unlike typica...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2013.06.041

    authors: Fisher D,Olasagasti I,Tank DW,Aksay ER,Goldman MS

    更新日期:2013-09-04 00:00:00

  • Synaptotagmins: That's Why So Many.

    abstract::Synaptotagmin 7 (Syt7) was originally identified as a slow Ca2+ sensor for lysosome fusion, but its function at fast synapses is controversial. The paper by Luo and Südhof (2017) in this issue of Neuron shows that at the calyx of Held in the auditory brainstem Syt7 triggers asynchronous release during stimulus trains,...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2017.05.011

    authors: Chen C,Jonas P

    更新日期:2017-05-17 00:00:00

  • An Optical Neuron-Astrocyte Proximity Assay at Synaptic Distance Scales.

    abstract::Astrocytes are complex bushy cells that serve important functions through close contacts between their processes and synapses. However, the spatial interactions and dynamics of astrocyte processes relative to synapses have proven problematic to study in adult living brain tissue. Here, we report a genetically targeted...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2018.03.003

    authors: Octeau JC,Chai H,Jiang R,Bonanno SL,Martin KC,Khakh BS

    更新日期:2018-04-04 00:00:00

  • Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

    abstract::The establishment of spinal motor neuron subclass diversity is achieved through developmental programs that are aligned with the organization of muscle targets in the limb. The evolutionary emergence of digits represents a specialized adaptation of limb morphology, yet it remains unclear how the specification of digit...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2017.01.017

    authors: Mendelsohn AI,Dasen JS,Jessell TM

    更新日期:2017-02-22 00:00:00

  • Epitranscriptomes in the Adult Mammalian Brain: Dynamic Changes Regulate Behavior.

    abstract::Epitranscriptomic modification of mRNA affects its metabolism and has recently been shown to regulate brain development. Two studies in this issue of Neuron, Koranda et al. (2018) and Engel et al. (2018), uncover dynamic and critical roles of m6A/m RNA modifications in the adult mammalian brain in regulating physiolog...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2018.07.019

    authors: Yoon KJ,Ming GL,Song H

    更新日期:2018-07-25 00:00:00

  • Rapid actin-based plasticity in dendritic spines.

    abstract::Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with g...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80467-5

    authors: Fischer M,Kaech S,Knutti D,Matus A

    更新日期:1998-05-01 00:00:00

  • Spike-time-dependent plasticity and heterosynaptic competition organize networks to produce long scale-free sequences of neural activity.

    abstract::Sequential neural activity patterns are as ubiquitous as the outputs they drive, which include motor gestures and sequential cognitive processes. Neural sequences are long, compared to the activation durations of participating neurons, and sequence coding is sparse. Numerous studies demonstrate that spike-time-depende...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2010.02.003

    authors: Fiete IR,Senn W,Wang CZ,Hahnloser RH

    更新日期:2010-02-25 00:00:00

  • BDNF mRNA is decreased in the hippocampus of individuals with Alzheimer's disease.

    abstract::In recent years, nerve growth factor (NGF) has gained attention as a potential therapeutic agent for Alzheimer's disease (AD). To study the expression of NGF and its homologs, brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3), postmortem samples of hippocampus from AD and control donors were examined ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(91)90273-3

    authors: Phillips HS,Hains JM,Armanini M,Laramee GR,Johnson SA,Winslow JW

    更新日期:1991-11-01 00:00:00

  • Inhibitory Basal Ganglia Inputs Induce Excitatory Motor Signals in the Thalamus.

    abstract::Basal ganglia (BG) circuits orchestrate complex motor behaviors predominantly via inhibitory synaptic outputs. Although these inhibitory BG outputs are known to reduce the excitability of postsynaptic target neurons, precisely how this change impairs motor performance remains poorly understood. Here, we show that opto...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2017.08.028

    authors: Kim J,Kim Y,Nakajima R,Shin A,Jeong M,Park AH,Jeong Y,Jo S,Yang S,Park H,Cho SH,Cho KH,Shim I,Chung JH,Paik SB,Augustine GJ,Kim D

    更新日期:2017-08-30 00:00:00

  • Response and habituation of the human amygdala during visual processing of facial expression.

    abstract::We measured amygdala activity in human volunteers during rapid visual presentations of fearful, happy, and neutral faces using functional magnetic resonance imaging (fMRI). The first experiment involved a fixed order of conditions both within and across runs, while the second one used a fully counterbalanced order in ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80219-6

    authors: Breiter HC,Etcoff NL,Whalen PJ,Kennedy WA,Rauch SL,Buckner RL,Strauss MM,Hyman SE,Rosen BR

    更新日期:1996-11-01 00:00:00

  • Rapid remodeling of retinal arbors in the tectum with and without blockade of synaptic transmission.

    abstract::Dynamic rearrangements of axon terminal arbors may be critical for establishing appropriate connections in the developing nervous system. Here, the changes in complex retinal axon arbors in the tecta of live Xenopus larvae were followed during the formation of the topographic retinotectal projection. Three-dimensional...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(94)90343-3

    authors: O'Rourke NA,Cline HT,Fraser SE

    更新日期:1994-04-01 00:00:00

  • Disruption of RNA Metabolism in Neurological Diseases and Emerging Therapeutic Interventions.

    abstract::RNA binding proteins are critical to the maintenance of the transcriptome via controlled regulation of RNA processing and transport. Alterations of these proteins impact multiple steps of the RNA life cycle resulting in various molecular phenotypes such as aberrant RNA splicing, transport, and stability. Disruption of...

    journal_title:Neuron

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuron.2019.03.014

    authors: Nussbacher JK,Tabet R,Yeo GW,Lagier-Tourenne C

    更新日期:2019-04-17 00:00:00

  • Waking State: Rapid Variations Modulate Neural and Behavioral Responses.

    abstract::The state of the brain and body constantly varies on rapid and slow timescales. These variations contribute to the apparent noisiness of sensory responses at both the neural and the behavioral level. Recent investigations of rapid state changes in awake, behaving animals have provided insight into the mechanisms by wh...

    journal_title:Neuron

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuron.2015.09.012

    authors: McGinley MJ,Vinck M,Reimer J,Batista-Brito R,Zagha E,Cadwell CR,Tolias AS,Cardin JA,McCormick DA

    更新日期:2015-09-23 00:00:00