Abstract:
:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the known C region gene as well as with different oligonucleotides specific for JH gene segments. Southern blot hybridization analysis identified a cluster of JH gene segments which are closely linked to the CH gene. Sequence analysis of the CH-proximal JH element, located about 1.9 kb upstream from the CH1 domain, showed that this element contains 5'-recombination signals typical of JH elements defined in higher vertebrates, i.e. a nonamer, a 24 bp spacer, and a heptamer. The coding region of this JH element was identical to that contained in the variable region sequence of a cDNA clone previously reported. Sequence analysis of the catfish JH-CH intron suggests that several sequences are present which appear similar to important transcriptional regulatory elements found within JH-CH introns of higher vertebrates. These features include sequences similar to higher vertebrate enhancer elements and regulatory octamers. An additional feature reminiscent of some higher vertebrate heavy chain switch regions is a repetitive sequence area composed of tandemly repeated simple sequences. Lastly, several restriction length polymorphisms were identified and mapped within a 1 kb region located immediately upstream from the JH cluster. This finding suggests that polymorphisms within the IgH locus should be useful in the analyses of channel catfish populations. These combined studies provide further evidence that the genomic organization of heavy chain genes in bony fish shares common organizational features with those known from higher vertebrates.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Ghaffari SH,Lobb CJdoi
10.1016/0161-5890(92)90096-gsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
151-9issue
2eissn
0161-5890issn
1872-9142journal_volume
29pub_type
杂志文章abstract::We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90167-8
更新日期:1994-06-01 00:00:00
abstract::The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90126-k
更新日期:1990-10-01 00:00:00
abstract::Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of I...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90062-7
更新日期:1983-03-01 00:00:00
abstract::The KH type splicing regulatory protein (KSRP) is a nucleic acid binding protein, which negatively regulates the stability and/or translatability of many mRNA species encoding immune-relevant proteins. As KSRP is expressed in immune cells including T and B cells, neutrophils, macrophages and dendritic cells, we wanted...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.05.003
更新日期:2017-07-01 00:00:00
abstract::The transglutaminase 2 (TG2) is one of the enigmatic enzymes with important functional diversity. It plays an important role in several pathologies such as celiac disease (CD). In patients with active CD, the abnormal retrotranscytosis of IgA/gliadin complexes is mediated by Transferrin Receptor 1 (TfR1). This triad a...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2020.02.019
更新日期:2020-05-01 00:00:00
abstract::Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. MAb COS3A could inhibit CD3-mediated T-c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.09.005
更新日期:2019-10-01 00:00:00
abstract::Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90088-2
更新日期:1991-01-01 00:00:00
abstract::Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.11.015
更新日期:2005-05-01 00:00:00
abstract::A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90159-9
更新日期:1988-11-01 00:00:00
abstract::We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid se...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.09.028
更新日期:2008-03-01 00:00:00
abstract::Host-derived macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infection, especially in malarial anemia. Although two Plasmodium parasite-derived MIF orthologs, Plasmodium falciparum MIF and P. berghei MIF were identified recently, the crystal structure and the precise rol...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.10.037
更新日期:2010-01-01 00:00:00
abstract::A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal p...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90123-9
更新日期:1986-09-01 00:00:00
abstract::We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90047-2
更新日期:1992-09-01 00:00:00
abstract::Recently, basophils have been suggested to produce inflammatory mediators in response to IgE in the absence of allergens. Monocyte chemoattractant protein-1 (MCP-1) plays an important role in the initiation of inflammatory responses by recruiting various immune cells to the site of allergic inflammation. In the presen...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.06.008
更新日期:2014-11-01 00:00:00
abstract::During the last years, intensive research has shed light in the transcriptional networks that shape the invariant NKT (iNKT) cell lineage and guide the choices towards functionally distinct iNKT cell subsets (Constantinides and Bendelac, 2013; Engel and Kronenberg, 2014; Gapin, 2016; Kim et al., 2015). However, the ep...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.08.020
更新日期:2018-09-01 00:00:00
abstract::Integrin-mediated adhesion of human monocytes to fibrinogen regulated by CD11b/CD18 and the closely related integrin CD11c/CD18, play a key role in inflammation. Peripheral blood monocytes isolated from human donors despite expressing CD11c primarily utilized CD11b to mediate adhesion to fibrinogen upon stimulation wi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.04.021
更新日期:2008-08-01 00:00:00
abstract::The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined B...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.02.021
更新日期:2019-05-01 00:00:00
abstract::The carbohydrate binding properties of a novel member of the subfamily of galactose-specific jacalin-related lectin isolated from the bark of black mulberry (Morus nigra) (Morniga G) was studied in detail by enzyme-linked lectinosorbent and inhibition assays using panels of monomeric saccharides, mammalian polyvalent ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.02.017
更新日期:2007-01-01 00:00:00
abstract::Reduced expression of Erythrocyte Complement Receptor 1 (E-CR1) is envisaged to contribute significantly to the pathophysiology of systemic lupus erythematosus (SLE). We determined the levels of CR1 transcript in the neutrophils from 25 untreated patients with active SLE and 25 normal healthy individuals and, studied ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.07.300
更新日期:2007-03-01 00:00:00
abstract::The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.07.010
更新日期:2010-11-01 00:00:00
abstract::Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four d...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90032-1
更新日期:1989-08-01 00:00:00
abstract::Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90089-7
更新日期:1987-02-01 00:00:00
abstract::Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90057-6
更新日期:1989-12-01 00:00:00
abstract::Major histocompatibility complex class I proteins (MHC-I) load short peptides derived from proteolytic cleavage of endogenous proteins in any cell of the body, in a process termed antigen processing and presentation. When the source proteins are altered self or encoded by a pathogen, recognition of peptide/MHC-I compl...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2015.04.014
更新日期:2015-12-01 00:00:00
abstract::Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.04.023
更新日期:2008-01-01 00:00:00
abstract::Antipeptide antisera were raised to the second and third complementarity-determining regions of the light chain derived from a human monoclonal IgM (Sie) which had antigammaglobulin activity and belonged to the Wa cross-reactive idiotypic group of human rheumatoid factors, two of whose members (Sie, Wo1) had been prev...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90049-0
更新日期:1986-03-01 00:00:00
abstract::The two N-linked oligosaccharides in native human C9 were deleted by site-specific mutagenesis. This aglycosyl-C9 did not differ from its native form in hemolytic and bactericidal activity. A new N-glycosylation site (K311N/E313T) was introduced into the turn of a helix-turn-helix [HTH] fold that had been postulated t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.01.013
更新日期:2010-04-01 00:00:00
abstract::The binding of CD40 ligand on activated T cells to CD40 on resting B cells induces the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The induction of B7 molecules by CD40 ligand-CD40 interaction represents a critical step in rendering B cells competent for antigen presentation. The CBA/N mouse has...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(96)00005-3
更新日期:1996-04-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) cells of a monocyte-macrophage lineage (rtMOCs) were used to characterize the ability of the trout innate immune system to recognize and respond to different pathogen associated molecular pattern (PAMP) bearing substances. Compared to what has been reported for mammalian macrophages...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.11.023
更新日期:2005-06-01 00:00:00
abstract::Intercellular adhesion molecule-1 (ICAM-1) functions in leukocyte trafficking, activation, and the formation of the immunological synapse. ICAM-1 is a member of the immunoglobulin superfamily of adhesion proteins, which share a similar structure of repeating Ig-like domains. Many genes in this family, including ICAM-1...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.03.005
更新日期:2010-05-01 00:00:00