Abstract:
:Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, acetaldehyde and iron. The conversion of C5 to a functionally active species was iron-dependent and inhibited by hydroxyl radical scavengers such as DMSO. The findings suggest that OH. is the active oxygen species that converts C5. The conversion product of C5, termed C5(H2O2), is C5b-like due to its ability to bind C6 and cause reactive lysis. C5(H2O2) is much more stable than C5b obtained by complement convertases. Although C5(H2O2) has lost the binding site of native C5 for C3b it can be cleaved by complement-derived convertases; the cleavage is, however, less efficient than in the case of native C5. The resulting cleavage product, which is C5a-like, is chemotactic although C5(H2O2) is not chemotactic. C5(H2O2) serves as a better substrate for plasma kallikrein than native C5, resulting in the generation of a C5a-like chemotactic product. These data indicate that oxygen radicals can bring about a conformational change in C5, causing it to behave as a functionally activated molecule of the complement system. This may have implications for the role of complement and its activation in the inflammatory response.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Vogt W,Damerau B,von Zabern I,Nolte R,Brunahl Ddoi
10.1016/0161-5890(89)90057-6subject
Has Abstractpub_date
1989-12-01 00:00:00pages
1133-42issue
12eissn
0161-5890issn
1872-9142journal_volume
26pub_type
杂志文章abstract::Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(96)00068-5
更新日期:1996-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90038-x
更新日期:1983-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2014.03.013
更新日期:2015-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00107-7
更新日期:1996-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90029-9
更新日期:1983-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1988-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.11.007
更新日期:2021-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00134-4
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/0161-5890(92)90001-e
更新日期:1992-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.09.005
更新日期:2016-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00022-1
更新日期:2003-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.10.009
更新日期:2015-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00090-1
更新日期:2002-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90144-1
更新日期:1987-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.04.010
更新日期:2004-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.11.015
更新日期:2005-05-01 00:00:00
abstract::Immunoglobulin heavy chain (Igh) locus rearrangements are controlled in part by an approximately 30 b complex 3' regulatory region located 3' of C alpha: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3' regulatory region and further downstream sequences from mous...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.09.006
更新日期:2005-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90001-0
更新日期:1987-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90126-0
更新日期:1989-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90081-2
更新日期:1987-02-01 00:00:00
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journal_title:Molecular immunology
pub_type:
doi:10.1016/s0161-5890(02)00203-1
更新日期:2003-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.06.013
更新日期:2013-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.05.007
更新日期:2012-10-01 00:00:00