Abstract:
:Host-derived macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infection, especially in malarial anemia. Although two Plasmodium parasite-derived MIF orthologs, Plasmodium falciparum MIF and P. berghei MIF were identified recently, the crystal structure and the precise roles of Plasmodium-derived MIFs, particularly in combination with the host MIF, remain unknown. In this study, we identified another MIF ortholog from a rodent-specific P. yoelii (PyMIF). This molecule shares a conserved three-dimensional structure with murine MIF (MmMIF), but with a different substrate binding pattern and much lower tautomerase activity. It could activate host cells via several signaling pathways in vitro, and inhibiting macrophage apoptosis, also similarly to MmMIF. However, we found that PyMIF and MmMIF acted synergistically to activate the MAPK-ERK1/2 signaling pathway at very low concentration but acted antagonistically at higher concentration. Furthermore, we detected PyMIF in the sera of infected mice and found that injection of recombinant PyMIF (rPyMIF) during infection could up-regulate several pro-inflammatory cytokines in vivo and slightly delay the death of infected mice. These data suggest that PyMIF modulates host immune responses together with host MIF and has potential to prolong parasitemia or the chronicity of malaria infection.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Shao D,Zhong X,Zhou YF,Han Z,Lin Y,Wang Z,Bu L,Zhang L,Su XD,Wang Hdoi
10.1016/j.molimm.2009.10.037subject
Has Abstractpub_date
2010-01-01 00:00:00pages
726-37issue
4eissn
0161-5890issn
1872-9142pii
S0161-5890(09)00816-5journal_volume
47pub_type
杂志文章abstract::Immature dendritic cells (iDCs) are bone marrow-derived professional antigen-presenting cells, exhibit very low levels of the co-stimulatory molecules CD80 (B7-1), CD86 (B7-2), and CD40 and major histocompatibility complex (MHC) class II and play a critical role in triggering antigen-specific immunotolerance. The enzy...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.09.005
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:The complement system is well known for its role in innate immunity via the classical, the alternative and the lectin pathways, although recent investigations suggest expanding roles in adipose tissue. Properdin stabilizes C3 convertase following alternative complement activation. Properdin is also present i...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.02.110
更新日期:2012-05-01 00:00:00
abstract::The variables regions of three syngeneic anti-idiotypic antibodies (Ab2s) were cloned and sequenced. They are encoded by different VL genes, two are from different members of V kappa-Ox1 superfamily. The H chains are encoded by VH genes belonging to three different VH families, J558, Q52 and 7183. Together with a prev...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90045-y
更新日期:1992-09-01 00:00:00
abstract::Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.09.025
更新日期:2015-12-01 00:00:00
abstract::The diagnosis and therapy of allergic disorders are usually performed with crude extracts which are a heterogeneous mixture of proteins with different allergenic potency. The knowledge of the allergenic composition is a key step for diagnostic and therapeutic options. Parietaria judaica pollen represents one of the ma...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.009
更新日期:2008-05-01 00:00:00
abstract::Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90030-2
更新日期:1994-10-01 00:00:00
abstract::The high affinity receptor for IgE, which mediates allergic reactions, is found exclusively on mast cells, basophils and epidermal Langerhans cells. It is composed of an alpha, beta and two gamma-polypeptide chains, inserted as a complex into the plasma membrane. The alpha and beta-subunits are produced only by these ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(96)84614-1
更新日期:1996-07-01 00:00:00
abstract::Bradykinin (BK) is an inflammatory mediator, and shows elevated levels in regions of severe injury and inflammatory diseases. It has been shown to induce interleukin-6 (IL-6) expression in inflammatory responses in rheumatoid arthritis. We investigated the signaling pathway involved in IL-6 production caused by BK in ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.06.007
更新日期:2008-08-01 00:00:00
abstract::By binding to T cell Ig mucin-3 (Tim-3) expressed on different cells, galectin-9 (Gal-9) mediates two important functions, triggering T cell death and activating innate immune cells. The mechanisms by which ligation of the same molecule on different cell types mediates different effects are largely unclear. Gal-9 cont...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.11.011
更新日期:2011-01-01 00:00:00
abstract::The carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus) was investigated by quantitative precipitin analysis using blood group A, B, H, Le and I substances and by precipitation inhibition with various mono- and oligosaccharides. The lectin precipitated best with A1 subst...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90101-6
更新日期:1989-02-01 00:00:00
abstract::The three-dimensional structure of the Mcg lambda-type Bence-Jones dimer crystallized in ammonium sulfate is known at 2.3-A resolution. A series of nine other human lambda-chains and two kappa-chains did not crystallize under the same conditions. After these proteins were hybridized with the Mcg light chain by the met...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(85)80001-8
更新日期:1985-02-01 00:00:00
abstract::Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90118-0
更新日期:1987-12-01 00:00:00
abstract::CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00055-8
更新日期:1998-06-01 00:00:00
abstract::To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90364-9
更新日期:1982-05-01 00:00:00
abstract::The role of glycosylation in membrane expression and secretion of IgM and IgA was investigated in murine lymphoma and hybridoma cell lines, derived from I.29 tumor, which synthesize IgM or IgA with identical variable regions. Tunicamycin, a selective inhibitor of N-linked glycosylation, prevented the membrane expressi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90023-3
更新日期:1984-08-01 00:00:00
abstract::SCID can be caused by various genetic mutations leading to distinctive phenotypes according to the presence of T, B and NK cells. Artemis is a gene encoded on chromosome 10p. The deficiency of this molecule causes an inability to repair DNA double strand breaks and is one of the causes of radiosensitive T-B-NK+ SCID. ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.05.004
更新日期:2013-12-01 00:00:00
abstract::Human neutrophil peptides (HNPs) are cysteine-rich antimicrobial peptides stored in neutrophils. The similar structure of HNPs -1, -2, and -3 renders them impossible to study individually in biological samples. For the first time, we describe a method of individually identifying the HNPs -1-3 from exudative neutrophil...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.08.001
更新日期:2006-03-01 00:00:00
abstract::Anaerobic reduction of purified rabbit IgG antibody (Ab) with 1.5 moles of dithiothreitol per mole of Ab at pH 8.0, followed by alkylation, cleaves 39% of the inter-heavy-chain (H-H) disulfide (SS) bonds. This treatment has the following effects on the ability of the Ab to activate the classical pathway of complement....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90092-0
更新日期:1984-01-01 00:00:00
abstract::Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90057-6
更新日期:1989-12-01 00:00:00
abstract::A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.10.018
更新日期:2016-01-01 00:00:00
abstract::The common variant in the human complement Factor H gene (CFH), with Tyr402His, is linked to age-related macular degeneration (AMD), a prevalent disorder leading to visual impairment and irreversible blindness in elderly patients. Here we show that the risk variant CFH 402His displays reduced binding to C reactive pro...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.012
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggeste...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.04.002
更新日期:2012-07-01 00:00:00
abstract::The alternative pathway of complement is receiving increasing attention as a therapeutic target because of recent findings in several animal models that support its essential role in tissue injury and disease pathogenesis. Although the contribution of alternative pathway activation to serum complement activation in vi...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.03.012
更新日期:2004-06-01 00:00:00
abstract::During the last years, intensive research has shed light in the transcriptional networks that shape the invariant NKT (iNKT) cell lineage and guide the choices towards functionally distinct iNKT cell subsets (Constantinides and Bendelac, 2013; Engel and Kronenberg, 2014; Gapin, 2016; Kim et al., 2015). However, the ep...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.08.020
更新日期:2018-09-01 00:00:00
abstract::A computerized method for predicting the locations of protein antigenic determinants is presented, which requires only the amino acid sequence of a protein, and no other information. This procedure has been used to predict the major antigenic determinant of the hepatitis B surface antigen, as well as antigenic sites o...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90029-9
更新日期:1983-04-01 00:00:00
abstract::Purified mRNA's encoding the HLA-A and -B antigen heavy chains or beta 2-microglobulin were prepared from human B lymphoid cells by positive hybridization selection procedures. The role of chain association in the biosynthesis and intracellular transport of HLA-A and -B antigens was investigated by injecting these mRN...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90115-x
更新日期:1986-05-01 00:00:00
abstract::Tehranolide named as new type of a sesquiterpene lactone with an endoperoxide group which purified from Artemisia diffusa and having similar structure to Artemisinin. Tehranolide is hypothesized to possess effects akin to Artemisinin, which is active against cancer cells. In the present research we emphasized on the d...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.01.007
更新日期:2010-04-01 00:00:00
abstract::BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2012.11.008
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The complement system plays an important role in the development of acute coronary syndrome (ACS). Complement C1q is an important initial component of the classical complement pathway and closely related to many chronic inflammatory diseases, including atherosclerosis (AS). We aimed to determi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.02.012
更新日期:2020-04-01 00:00:00
abstract::mRNA encoding secreted immunoglobulin is synthesized either by termination of transcription 3' to secreted terminus sequences and 5' to the membrane terminus sequences or by cleavage of a pre-mRNA transcript containing both secreted and membrane sequences at the appropriate polyadenylation site 5' to the membrane sequ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90110-6
更新日期:1991-07-01 00:00:00