Abstract:
:To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with the myeloma cell line M45. In a solid-phase ELISA assay to measure anti-DNA activity, Cll showed preference for single stranded compared to double stranded DNA of animal origin. The Cll antibody also bound some deoxyribohomopolymers as well as ribohomopolymers, but failed to bind synthetic DNA duplexes. Defined size oligonucleotides with a size range of 2-(12-18) failed to inhibit the binding of Cll to single stranded DNA. This pattern of binding is consistent with the recognition of a unique structural determinant that can be represented by a variety of nucleic acids. The absence of antigenic activity among the oligonucleotides suggests that an extended polynucleotide structure is required for antibody binding, possibly because of a bivalent or 'monogamous' mode of interaction. The binding properties of Cll further suggest that its ability to participate in immune complex formation may be limited by the nature of the available DNA antigen.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Pisetsky DS,Caster SAdoi
10.1016/0161-5890(82)90364-9subject
Has Abstractpub_date
1982-05-01 00:00:00pages
645-50issue
5eissn
0161-5890issn
1872-9142journal_volume
19pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00078-x
更新日期:2000-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.10.005
更新日期:2014-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.017
更新日期:2017-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.10.018
更新日期:2016-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00059-7
更新日期:2002-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.04.023
更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.01.006
更新日期:2010-04-01 00:00:00
abstract::The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injec...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.12.005
更新日期:2006-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.030
更新日期:2006-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90038-y
更新日期:1992-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1982-08-01 00:00:00
abstract:UNLABELLED:C5L2, a G-protein-coupled receptor (GPCR), has been identified as an ASP (C3adesArg) and C5a receptor. Controversy exists regarding both ligand binding and functionality. ASP activation of C5L2 is proposed to regulate fat storage. C5L2 is also proposed as a decoy receptor for C5a, an inflammatory mediator, b...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.06.007
更新日期:2009-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.02.007
更新日期:2017-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.08.015
更新日期:2014-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00108-4
更新日期:1998-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.020
更新日期:2006-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.07.006
更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(84)90143-3
更新日期:1984-10-01 00:00:00