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Comparison of the glycosylation of in vitro generated polyclonal human IgG and therapeutic immunoglobulins.

Abstract：

:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in this model. Switched-memory B cells were cultured for 38 days and, using liquid chromatography-mass spectrometry, we analyzed IgGs' glycosylation profiles which were then compared to the glycosylation patterns of commercial intravenous immunoglobulin (IVIG). We observed a reproducible proliferation rate, high viability through the cultures as well as a good maintenance of the switched-memory B cells repertoire. The glycosylation pattern analyses revealed a variety of the typical biantennary N-glycan structures with diverse terminal monosaccharides. While many similarities were detected in comparison to the glycosylation profile of IVIG, in vitro-produced polyclonal IgGs were bearing higher levels of bisecting GlcNAc known to affect the effector functions of therapeutic antibodies. This data highlights the need for monitoring of the glycoform distribution in antibodies produced in vitro.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Ritamo I,Cloutier M,Valmu L,Néron S,Räbinä J

doi

10.1016/j.molimm.2013.10.005

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

255-62

issue

2

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(13)00531-2

journal_volume

57

pub_type

杂志文章

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