Abstract:
:Mugwort (Artemisia vulgaris) belongs to the Compositae family, and is one of the main causes of allergy in late summer and autumn. The aim of the study was to characterize the allergen Art v 2 from mugwort pollen. Skin prick tests, performed in 19 patients allergic to mugwort and 10 control patients, showed an Art v 2 sensitization prevalence of 58%, whereas none false-positives were detected among control patients. Art v 2 was purified by standard chromatography and binding to Concanavalin A column and had an apparent molecular mass of 33 and 20 kDa, calculated by gel permeation and SDS-PAGE under denaturing conditions, respectively, showing that the allergen is composed of two identical subunits. Art v 2-encoding cDNA was amplified by PCR using degenerate primers based on reported partial amino acid sequences. Cloned cDNA encoding Art v 2 contains 140 bp that codify for a polypeptide of 15.8 kDa, with a predicted pI value of 5.2, and one potential N-glycosylation site. Protein homology search demonstrated that Art v 2 share 55-42% identical residues with pathogenesis-related protein PR-1 of tomato, potato, rape, wheat and rice. Homology was also found to Ves v 5 (41% identical residues). Bacterial-expressed recombinant Art v 2 was recognized only by 21% of mugwort-allergic patients. In conclusion, Art v 2 from mugwort is the first weed pollen allergen that belongs to the pathogenesis-related protein PR-1 and its recombinant form could help molecular diagnosis of mugwort associated allergy.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Arilla MC,Ibarrola I,Puente Y,Daza JC,Martínez A,Asturias JAdoi
10.1016/j.molimm.2007.04.022subject
Has Abstractpub_date
2007-07-01 00:00:00pages
3653-60issue
15eissn
0161-5890issn
1872-9142pii
S0161-5890(07)00162-9journal_volume
44pub_type
杂志文章abstract::In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.01.001
更新日期:2004-04-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) cells of a monocyte-macrophage lineage (rtMOCs) were used to characterize the ability of the trout innate immune system to recognize and respond to different pathogen associated molecular pattern (PAMP) bearing substances. Compared to what has been reported for mammalian macrophages...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.11.023
更新日期:2005-06-01 00:00:00
abstract::Forty-eight group A streptococcal strains of different M types were screened for binding of human radiolabeled IgG. Three of the strains bound more than 80% of the added radioactivity and one of them, an M protein type 1 strain designated AP1, was selected for further analysis. Attempts were made to solubilize the IgG...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90071-7
更新日期:1990-06-01 00:00:00
abstract::Protein kinase C-associated kinase (PKK; DIK/RIP4) is an ankyrin-repeat containing serine/threonine receptor-interacting protein (RIP)-family kinase that can activate NFkappaB, and is required for keratinocyte development. In earlier studies, the expression of a catalytically inactive mutant of PKK in the B cell linea...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.09.009
更新日期:2006-04-01 00:00:00
abstract::B cells play a critical role in immune responses both in physiological and pathological conditions, and microRNAs have been shown to play important roles in regulating B cell proliferation and function. MiR-146a has been shown to modulate T cell immunity, but its function in regulating B cell response remains partiall...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.10.023
更新日期:2017-12-01 00:00:00
abstract::In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90039-3
更新日期:1987-06-01 00:00:00
abstract::To test the fate of developing B cells with autoreactive receptor components, we studied mice homozygous for a knock-in transgene coding the VH domain of an IgM ssDNA-binding antibody. The transgene has unmutated C57 BL/6 V gene segments. Homozygous knock-in mice developed normal numbers of spleen and bone marrow B ce...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.01.010
更新日期:2005-08-01 00:00:00
abstract::Complement per se has been shown to play an important role in demyelinating disease but controversy remains regarding the role of C3 in the development and progression of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. In this study, we used C3(-/-) mice to confirm previous fi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.002
更新日期:2007-05-01 00:00:00
abstract::The Pms2 gene is involved in DNA mismatch repair in mammalian cells, and has recently been shown to affect hypermutation of mammalian immunoglobulin genes. We have studied hypermutation of a lambda1 transgene in chronically stimulated Peyer's patch B cells of both young and old mice deficient in function of Pms2. In y...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00027-9
更新日期:1999-02-01 00:00:00
abstract::The cell surface co-stimulatory protein CD154 (CD40L) is a target for monoclonal antibody (mAb) inhibitors of T-cell mediated immune diseases. This protein, like most other members of the TNF ligand family, forms homotrimeric complexes on the cell surface and in solution, with a three-fold axis of symmetry. We find th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00045-7
更新日期:2002-09-01 00:00:00
abstract::The accuracy of calibrated immunoassays for measuring antibody concentration was analysed from a theoretical perspective. The study shows that there are theoretical limits on the accuracy of antibody immunoassays, which are determined by the affinity of the standard and unknown antibodies and the conditions chosen for...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90115-z
更新日期:1991-07-01 00:00:00
abstract::The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 prove...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90124-g
更新日期:1992-07-01 00:00:00
abstract::The anti-inflammatory cytokine IL-10 is a key modulator of immune responses. A better understanding of the regulation of this cytokine offers the possibility of tipping the balance of the immune response toward either tolerance, or enhanced immune responses. Histone deacetylases (HDACs) have been widely described as n...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.02.019
更新日期:2014-07-01 00:00:00
abstract::Fcα/μR (CD351) is an Fc receptor for both IgA and IgM and forms an atypical dimer that is resistant to reduction by 2-mercaptoethanol or boiling. We previously demonstrated that the cytoplasmic portion of Fcα/μR is required for dimer formation and for its efficient cell-surface expression. However, the biochemical nat...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.04.003
更新日期:2013-11-01 00:00:00
abstract::In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90026-0
更新日期:1988-04-01 00:00:00
abstract::Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.09.017
更新日期:2019-12-01 00:00:00
abstract::Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients immunoreactive with topoisomerase I. All sera reacted selectively with ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90145-a
更新日期:1991-04-01 00:00:00
abstract::Hepatitis B virus (HBV) infection afflicts over 350 million people worldwide and is a leading cause of hepatitis, cirrhosis and hepatocellular carcinoma. HBV replicates noncytopathically in hepatocytes, and most of the hepatic injury is caused by the immune response to the virus. While most studies focused on the adap...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.022
更新日期:2009-12-01 00:00:00
abstract::Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.03.006
更新日期:2006-02-01 00:00:00
abstract::Comparative interactions of purified rabbit C1q with 18-2-3, a high affinity (2-3 X 10(10) M-1) anti-fluorescein (anti-F1) murine monoclonal IgM antibody (pentamer) and constitutive monomeric subunits (IgMs) were studied. Using a solid phase radioimmunoassay (SPRIA), based on immobilized polyvalent antigen, it was sho...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90076-4
更新日期:1988-06-01 00:00:00
abstract::Tumor-associated macrophages (TAMs) are preferentially M2 skewed and promote tumor growth, angiogenesis, invasion, and/or metastasis. In this study, we have analyzed the in vitro immunomodulatory potential of a non-toxic neem leaf glycoprotein (NLGP) in reprogramming Stage III supraglottic laryngeal tumor cell lysate ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.01.015
更新日期:2014-06-01 00:00:00
abstract::Increasing lines of evidence indicate that arsenic may be associated with immune related problems, but its detailed effects on immune organs are poorly understood. The objective of this study was to explore inflammatory responses and T cell differentiation of arsenic exposure in spleen and thymus. Female C57BL/6 mice ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.12.005
更新日期:2017-01-01 00:00:00
abstract::Lipopolysaccharide (LPS) present on the outer membrane of Gram-negative bacteria is one of the most important pathogen-associated molecular patterns and a potent elicitor in innate immunity. In human, TLR4 (Toll-like receptor 4) and MD-2 (myeloid differiation-2) form a receptor complex to transduce the LPS signal into...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.02.006
更新日期:2008-05-01 00:00:00
abstract::The functional importance of each amino acid residue making up the antigenic determinants of three different peptide-mAb interactions was determined using complete series of substitution analogs of the three immunizing synthetic peptides. Fingerprint substitution profiles for the three different antigenic determinants...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90032-7
更新日期:1993-04-01 00:00:00
abstract::Recent studies demonstrate that tissue microenvironments may regulate development and functions of immune cells including dendritic cells, monocytes and T cells. However, there is no report about functional regulation of innate NK cells by tissue microenvironment. The liver is an organ abundant in NK cells and suscept...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.035
更新日期:2008-05-01 00:00:00
abstract::The immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO) suppresses T-cell responses and promotes immune tolerance in tumor resistance. A previous study determined that IDO inhibits Vav1 mRNA expression and the activation of Vav1 and its downstream targets in T cells in the guanine exchange factor (GEF)-independe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.018
更新日期:2017-01-01 00:00:00
abstract::Type 2 Diabetes (T2D) is a disease of increasing importance and represents a growing burden on global healthcare and human health. In T2D, loss of effectiveness of insulin signaling in peripheral tissues cannot be compensated for by adequate insulin secretion, leading to hyperglycemia and resultant complications. In r...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2016.12.009
更新日期:2017-04-01 00:00:00
abstract::Dendritic cells (DCs) are recruited to inflammatory sites where they phagocytose and process antigens for subsequent presentation to the T lymphocytes in the lymphoid tissue. Several leukocyte chemoattractants and their specific receptors have been shown to induce the migration of DC. The complement protein C1q has mu...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.030
更新日期:2006-03-01 00:00:00
abstract::Surfactant protein D (SP-D) interacts with various different microorganisms and plays an important role in pulmonary innate immunity. SP-D expression has also been detected in extrapulmonary tissues, including the gastro-intestinal tract. However, its function in the intestine is unknown and may differ considerably fr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.03.013
更新日期:2007-07-01 00:00:00
abstract::The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined B...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.02.021
更新日期:2019-05-01 00:00:00