PD-L1+ regulatory B cells act as a T cell suppressor in a PD-L1-dependent manner in melanoma patients with bone metastasis.


:The five-year survival rate of melanoma worsens significantly with advancing tumor stage. We hypothesized that regulatory B cells (Breg) might have participated in the pathogenesis of melanoma. In this study, the PD-L1+ Breg cells were investigated. The expression of PD-L1 by circulating B cells was very low in healthy controls. In melanoma patients, on the other hand, the expression of PD-L1 by circulating B cells was significantly elevated in a manner that was positively associated with tumor stage, with the highest level in stage IV bone metastasis patients. Compared to total B cells, PD-L1+ B cells presented higher IgM and higher IgD expression, and were almost exclusively CD20+CD27-, suggesting that the PD-L1+ B cells exhibited a naive B cell-like phenotype. Healthy naive B cells, which presented little PD-L1, and stage I and stage II melanoma patient naive B cells, which presented detectable but low PD-L1, were unable to suppress T cell response. However, stage III and stage IV naive B cells, which presented moderate PD-L1, could significantly suppress T cell response in a PD-L1-dependent manner. We further found that the level of PD-L1+ B cells was significantly higher in bone metastasis than in the primary tumors. Overall, we demonstrated that PD-L1+ B cells were upregulated in advanced melanoma and were enriched in metastasis compared to primary tumors. Furthermore, PD-L1+ naive B cells could act as a T cell suppressor in a PD-L1-dependent manner.


Mol Immunol


Molecular immunology


Wu H,Xia L,Jia D,Zou H,Jin G,Qian W,Xu H,Li T




Has Abstract


2020-03-01 00:00:00












  • Serologic and molecular characterization of the T15 idiotype--II. Structural basis of independent idiotope expression on phosphorylcholine-specific monoclonal antibodies.

    abstract::We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Strickland FM,Gleason JT,Cerny J

    更新日期:1987-06-01 00:00:00

  • MKK6 from pacific white shrimp Litopenaeus vannamei is responsive to bacterial and WSSV infection.

    abstract::p38 mitogen-actived protein kinases (MAPKs) broadly exist from yeast to mammals and participate in diverse cellular responses to various stimuli, whose activation can be induced by the MAPK kinase 6 (MKK6). In this study, a novel MKK6 homolog from Litopenaeus vannamei (LvMKK6) was cloned and characterized. The transcr...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li H,Wang S,Qian Z,Wu Z,Lǚ K,Weng S,He J,Li C

    更新日期:2016-02-01 00:00:00

  • Variants in the promoter region of IKBL/NFKBIL1 gene may mark susceptibility to the development of chronic Chagas' cardiomyopathy among Trypanosoma cruzi-infected individuals.

    abstract::Chagas' disease, caused by Trypanosoma cruzi, is an inflammatory disorder leading to chronic Chagas cardiomyopathy (CCC). Only one third of T. cruzi-infected individuals progress to CCC while the others are considered asymptomatic (ASY). The human inhibitory kappaB-like gene (IKBL/NFKBIL1), homologous to the IkappaB f...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Ramasawmy R,Faé KC,Cunha-Neto E,Borba SC,Ianni B,Mady C,Goldberg AC,Kalil J

    更新日期:2008-01-01 00:00:00

  • Comparative analysis of human and mouse 3' Igh regulatory regions identifies distinctive structural features.

    abstract::Immunoglobulin heavy chain (Igh) locus rearrangements are controlled in part by an approximately 30 b complex 3' regulatory region located 3' of C alpha: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3' regulatory region and further downstream sequences from mous...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sepulveda MA,Garrett FE,Price-Whelan A,Birshtein BK

    更新日期:2005-03-01 00:00:00

  • Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries.

    abstract::Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Goodchild SA,Dooley H,Schoepp RJ,Flajnik M,Lonsdale SG

    更新日期:2011-09-01 00:00:00

  • The heterogeneity of bovine IgG2--IV. Structural differences between IgG2a molecules of the A1 and A2 allotypes.

    abstract::The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement o...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Heyermann H,Butler JE

    更新日期:1987-12-01 00:00:00

  • Circular Dichroism reveals evidence of coupling between immunoglobulin constant and variable region secondary structure.

    abstract::Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Janda A,Casadevall A

    更新日期:2010-04-01 00:00:00

  • Elicitation of distinct populations of monoclonal antibodies specific for the variable domains of monoclonal anti-fluorescein antibody 4-4-20.

    abstract::Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...

    journal_title:Molecular immunology

    pub_type: 杂志文章


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    更新日期:1993-08-01 00:00:00

  • Atypical aHUS: State of the art.

    abstract::Tremendous advances in our understanding of the thrombotic microangiopathies (TMAs) have revealed distinct disease mechanisms within this heterogeneous group of diseases. As a direct result of this knowledge, both children and adults with complement-mediated TMA now enjoy higher expectations for long-term health. In t...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Nester CM,Barbour T,de Cordoba SR,Dragon-Durey MA,Fremeaux-Bacchi V,Goodship TH,Kavanagh D,Noris M,Pickering M,Sanchez-Corral P,Skerka C,Zipfel P,Smith RJ

    更新日期:2015-09-01 00:00:00

  • NF-kappaB family of transcription factor facilitates gene conversion in chicken B cells.

    abstract::Activation-induced cytidine deaminase (AID) is critical for immunoglobulin (Ig) diversification in B cells. The majority of evidence supports the model that AID modifies Ig genes at the DNA level by deaminating cytosines into uracils. The mutagenic activity is largely restricted to Ig genes to avoid genomic damage in ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kim Y,Tian M

    更新日期:2009-10-01 00:00:00

  • Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).

    abstract::An initial bioinformatics investigation followed by cloning and sequencing analysis, has led to the identification of three novel members (omDB-2, omDB-3, omBD-4) of the beta-defensin family in rainbow trout (Oncorhynchus mykiss). The contiguous sequences could be translated to give predicted peptides of 62 (omDB-2), ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Casadei E,Wang T,Zou J,González Vecino JL,Wadsworth S,Secombes CJ

    更新日期:2009-10-01 00:00:00

  • Comparative research on nucleocapsid and spike glycoprotein as the rapid immunodetection targets of COVID-19 and establishment of immunoassay strips.

    abstract::The SARS-CoV-2 virus responsible for coronavirus 2019 (COVID-19) poses a significant challenge to healthcare systems worldwide. According to the World Health Organization (WHO), the outbreak of COVID-19 has been a pandemic that infected more than 25.32 million people and caused more than 848.25 thousand deaths worldwi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Liu D,Wu F,Cen Y,Ye L,Shi X,Huang Y,Fang S,Ma L

    更新日期:2021-01-09 00:00:00

  • Unique biochemical properties of a mutant MHC class I molecule, H-2Ksm1.

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    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Hasenkrug KJ,Kesari KV,Nathenson SG

    更新日期:1992-12-01 00:00:00

  • Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis.

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    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chen Y,Duan R,Li X,Li K,Liang J,Liu C,Qiu H,Xiao Y,Jing H,Wang X

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  • Are the decrease in circulating anti-α1,3-Gal IgG and the lower content of galactosyl transferase A1 in the microbiota of patients with multiple sclerosis a novel environmental risk factor for the disease?

    abstract::The etiology of multiple sclerosis (MS), particularly the environmental component of the disease, remains speculative. Recent reports have suggested that alterations in the gut microbiota of MS patients could contribute to the etiology or pathophysiology of the disease. In this Viewpoint, using PICRUSt (Phylogenetic I...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Montassier E,Berthelot L,Soulillou JP

    更新日期:2018-01-01 00:00:00

  • Major mountain cedar allergen, Jun a 1, contains conformational as well as linear IgE epitopes.

    abstract::We have previously identified four linear IgE epitopes on Jun a 1, the dominant allergen in mountain cedar pollen, and mapped these to the surfaces of a molecular model and to the crystal structure of this glycoprotein. The aim of the present study was to determine if Jun a 1 also displays conformational IgE epitopes....

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Varshney S,Goldblum RM,Kearney C,Watanabe M,Midoro-Horiuti T

    更新日期:2007-04-01 00:00:00

  • Preparation and characterization of a disulfide-stabilized Fv fragment of the anti-Tac antibody: comparison with its single-chain analog.

    abstract::Recombinant DNA techniques now allow the production of "mini-antibodies" called Fv fragments. These have been produced either as the independent variable domains of the heavy and light chains non-covalently associated in one-to-one stoichiometry or as single-chain gene products with the two domains linked by an interv...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Webber KO,Reiter Y,Brinkmann U,Kreitman R,Pastan I

    更新日期:1995-03-01 00:00:00

  • Receptors for complement C3 on T-lymphocytes: relics of evolution or functional molecules?

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    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Wagner C,Hänsch GM

    更新日期:2006-01-01 00:00:00

  • Characterization of human complement components C6 and C7.

    abstract::Human complement components C6 and C7 have been purified and characterized. Component C6 is a single-chain plasma protein of mol. wt 104,800 containing 3.8% carbohydrate and it has alanine as the amino terminal residue. Component C7 is a single-chain plasma protein of mol. wt 92,400 containing 6.4% carbohydrate and it...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Discipio RG,Gagnon J

    更新日期:1982-11-01 00:00:00

  • Definition of the peptide binding motif within DRB1*1401 restricted epitopes by peptide competition and structural modeling.

    abstract::This study identified the peptide-binding motif of HLA-DRA/DRB1*1401 (DR1401). First, peptides containing DR1401 restricted epitopes were identified using tetramer-guided epitope mapping. Among these, an influenza B peptide was selected for the motif study. After confirming the binding register for this peptide using ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: James EA,Moustakas AK,Berger D,Huston L,Papadopoulos GK,Kwok WW

    更新日期:2008-05-01 00:00:00

  • Concanavalin A induces a cytoskeletal association of T200 molecules in T lymphocytes.

    abstract::A recent report indicated that T200 molecules interact with elements of the cytoskeleton in BW5147 T lymphoma cells. We have confirmed the cytoskeletal association of T200 by examining nonionic detergent-soluble and detergent-insoluble fractions of murine T cell tumor cell lines, cloned cytotoxic T lymphocyte lines, a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Taffs RE,Ewald SJ

    更新日期:1989-10-01 00:00:00

  • The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways.

    abstract::The T cell receptor (TCR) detects the presence of infectious pathogens and activates numerous intracellular signaling pathways. Protein tyrosine phosphorylation and ubiquitination serve as key regulatory mechanisms downstream of the TCR. Negative regulation of TCR signaling pathways is important in controlling the imm...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Carpino N,Chen Y,Nassar N,Oh HW

    更新日期:2009-10-01 00:00:00

  • Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene.

    abstract::In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10(-6) M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Unterberger C,Staples KJ,Smallie T,Williams L,Foxwell B,Schaefer A,Kempkes B,Hofer TP,Koeppel M,Lohrum M,Stunnenberg H,Frankenberger M,Ziegler-Heitbrock L

    更新日期:2008-06-01 00:00:00

  • Studies of the antigenic structure of two cross-reacting proteins, pertussis and cholera toxins, using synthetic peptides.

    abstract::Peptide fragments of pertussis toxin subunit 1 (PT-S1) have been synthesized in order to investigate their antigenic and immunogenic activity, and to evaluate their possible use as components of a new vaccine. Two peptides (sequence 73-82, EAERAGRGTG and sequence 107-116, YVDTYGDNAG) were selected for their predictabl...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Presentini R,Perin F,Ancilli G,Nucci D,Bartoloni A,Rappuoli R,Antoni G

    更新日期:1989-01-01 00:00:00

  • H-2Ld-alloreactive T cell hybridomas utilize diverse V alpha and V beta T cell receptor chains.

    abstract::We have sequenced the TCRs from Ld-specific alloreactive T cell hybridomas, whose reactivities we have found to be quite representative of those of a primary dm2 anti-BALB/cJ mixed lymphocyte reaction. We find V beta 6, V beta 7, V beta 8 and V beta 10 gene segments. V alpha usage is diverse, although closely related ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Louie KA,Ochoa-Garay J,Chen PJ,McKinney D,Groshen S,McMillan M

    更新日期:1996-06-01 00:00:00

  • IVIG immunotherapy protects against synaptic dysfunction in Alzheimer's disease through complement anaphylatoxin C5a-mediated AMPA-CREB-C/EBP signaling pathway.

    abstract:BACKGROUND:Complement component C5-derived C5a locally generated in the brain has been shown to protect against glutamate-induced neuronal apoptosis and beta-amyloid (Aβ) toxicity, but the mechanism is not clear. In this study, we tested the hypothesis that C5a influences upstream signal transduction pathways associate...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gong B,Pan Y,Zhao W,Knable L,Vempati P,Begum S,Ho L,Wang J,Yemul S,Barnum S,Bilski A,Gong BY,Pasinetti GM

    更新日期:2013-12-01 00:00:00

  • Germline transcripts of immunoglobulin light chain variable regions are structurally diverse and differentially expressed.

    abstract::The murine pre-B cell line R2-bfl, which can be induced to differentiate in vitro, was used to study germline transcription of variable regions of the light chain loci. RNA from these cells was subjected to a 3'-RACE and germline transcripts from 17 individual Vkappa gene segments belonging to 12 Vkappa families were ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Düber S,Engel H,Rolink A,Kretschmer K,Weiss S

    更新日期:2003-12-01 00:00:00

  • Functional analysis of naturally occurring amino acid substitutions in human IFN-gammaR1.

    abstract::IFN-gamma plays an essential role in the IL-12/IL-23/IFN-gamma pathway that is required for the defense against intracellular pathogens. In the IFN-gammaR1 several amino acid substitutions have been reported that abrogate IFN-gamma signaling. These substitutions can lead to a null phenotype and enhanced susceptibility...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: van de Wetering D,de Paus RA,van Dissel JT,van de Vosse E

    更新日期:2010-02-01 00:00:00

  • Pre-thymic transcription of TCR genes by adult murine bone marrow cells.

    abstract::In the adult mouse, the earliest thymocytes are derived from bone marrow-resident T lymphocyte precursor (pre-T) cells that immigrate to the thymus. There they undergo maturation through a series of developmental steps that include rearrangement and expression of the TCR genes, positive and negative selection, and fun...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wang TG,Lybarger L,Soloff R,Dempsey D,Chervenak R

    更新日期:1996-08-01 00:00:00

  • Antibody repertoire development in fetal and neonatal piglets. V. VDJ gene chimeras resembling gene conversion products are generated at high frequency by PCR in vitro.

    abstract::The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Sinkora M,Sun J,Butler JE

    更新日期:2000-12-01 00:00:00