Abstract:
:Mice were immunized with a purified preparation of human alpha-foetoprotein (AFP) and their spleen cells were hybridized with mouse myeloma cells. Eleven hybridoma cell lines secreting antibody to AFP were obtained. These antibodies were classified into two groups on the basis of different antigenic determinants they recognized. Seven cell lines produced antibodies directed to one determinant of AFP (determinant a), while the remaining 4 produced antibodies to another determinant (determinant b). A native AFP molecule bore one each of a and b determinants which were accessible by monoclonal antibodies; it was detected by a sandwich-type solid-phase radioimmunoassay only when antibody to a was used for coating wells of the microtitre plate and antibody to b as radiolabelled reagent, or vice versa. The presence of two different antigenic determinants on AFP was further confirmed by a chemical modification. When AFP was reduced in the presence of 2 mM dithiothreitol and then alkylated, determinant b was completely destroyed, but the determinant a remained unaffected. Furthermore, reduced and alkylated AFP was detected by the radioimmunoassay employing antibody to a both for coating wells and as radiolabelled reagent, thereby indicating that it bore two determinant as, one of which had been unaccessible in the native AFP.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Nomura M,Imai M,Nakamura T,Miyakawa Y,Mayumi Mdoi
10.1016/0161-5890(82)90281-4subject
Has Abstractpub_date
1982-12-01 00:00:00pages
1691-7issue
12eissn
0161-5890issn
1872-9142journal_volume
19pub_type
杂志文章abstract::Induction of the TCR signaling pathway terminates the expression of RAG genes, and a link between this pathway and their transcriptional control is evident from the recent demonstration of their re-expression if the TCR is subsequently lost or down-regulated. Since unstimulated T cells display a steady-state level of ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.02.015
更新日期:2011-07-01 00:00:00
abstract::Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90162-q
更新日期:1992-01-01 00:00:00
abstract::Inhibition of C5a by antibodies has been demonstrated to dramatically improve survival in various sepsis models in mice and rats. The structural basis of C5a mediated bioactivity and C5a antibody mediated neutralization are of interesting to be investigated. In the previous study, we obtained a novel functional mouse ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.03.013
更新日期:2011-07-01 00:00:00
abstract::Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.025
更新日期:2007-03-01 00:00:00
abstract::Hepatitis B virus (HBV) infection afflicts over 350 million people worldwide and is a leading cause of hepatitis, cirrhosis and hepatocellular carcinoma. HBV replicates noncytopathically in hepatocytes, and most of the hepatic injury is caused by the immune response to the virus. While most studies focused on the adap...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.022
更新日期:2009-12-01 00:00:00
abstract::Chlorophyllin (CHL) was earlier shown to reduce the level of intracellular ROS and apoptosis induced by ionizing radiation and 2,2'-azobis(2-propionimidinedihydrochloride) (AAPH). In the present studies, the effect of CHL on radiation-induced immunosuppression and modulation of immune responses in mice was examined. C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.02.031
更新日期:2007-01-01 00:00:00
abstract::Amyloid-beta (Aβ) plaque accumulation in the brain is one of the hallmarks of Alzheimer's disease (AD). Immunotherapy against Aβ was considered a potential strategy for reducing the Aβ load in the brain. However, none of the Aβ immunotherapies have produced clinically meaningful results to date, due to poor safety or ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.019
更新日期:2018-09-01 00:00:00
abstract::Mastocytosis is an incurable and sometimes fatal haematological disorder grossly described as the accumulation of abnormal mast cells in the bone marrow and other organs causing tissue and organ damage. The clinical manifestations of this disease are extremely variable; disease phenotypes range from indolent to aggres...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2014.03.013
更新日期:2015-01-01 00:00:00
abstract::V(D)J recombination is a mechanism peculiar to the somatic rearrangement of antigen receptor genes. It requires both expression of the RAG-1 and RAG-2 recombinases and accessibility of the substrate to its recombinase and post-cleavage/DNA repair stage. TCR revision is a genetic correction mechanism that changes T cel...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.06.153
更新日期:2008-01-01 00:00:00
abstract::Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90139-2
更新日期:1994-01-01 00:00:00
abstract::Interleukin 10 (IL-10)-producing regulatory B-cells (Bregs) suppress inflammatory responses that mediate autoimmune diseases. However, it is unknown whether Bregs derive from a pre-existing dedicated B-cell lineage or if any B-cell can differentiate into Bregs in response to BCR or TLR activation. GL7(+) B-cells are a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.01.011
更新日期:2016-03-01 00:00:00
abstract::Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90089-7
更新日期:1987-02-01 00:00:00
abstract::Placental growth factor (PlGF), a member of vascular endothelial growth factors (VEGF) family, is considered as an important antigen associated with pathological conditions such as cancer cell growth, and metastasis. PlGF-targeting via nanobody (Nb) therefore could be beneficial to modulate these pathologies. In this ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.09.012
更新日期:2016-10-01 00:00:00
abstract::HLA-B27 (B27) is strongly associated with the spondyloarthritides. B27 is expressed at the cell surface of antigen presenting cells (APC) both as canonical β2m-associated and non-canonical β2m-free heavy chain (FHC) forms which include B27 dimers (termed B272). B27 FHC forms arise in an endosomal compartment from recy...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.05.243
更新日期:2014-01-01 00:00:00
abstract::With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.004
更新日期:2006-04-01 00:00:00
abstract::The NLRP3 inflammasome constitutes a major antiviral host defense mechanism during influenza virus infection. Inflammasome assembly in virus-infected cells facilitates autocatalytic processing of pro-caspase-1 and subsequent cleavage and secretion of proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.01.023
更新日期:2017-06-01 00:00:00
abstract::Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.006
更新日期:2017-11-01 00:00:00
abstract::In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.01.001
更新日期:2004-04-01 00:00:00
abstract::Asthma is a complex condition in which exposure to environmental antigens induces inflammatory reactions in the airway characterized by activation of mast cells and eosinophils. Mast cells are known to be the main effector cells in eliciting IgE-mediated allergic response. These cells secrete various substances that p...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00070-6
更新日期:2002-09-01 00:00:00
abstract::The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containin...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.12.007
更新日期:2010-03-01 00:00:00
abstract::Signal transduction by the B cell receptor (BCR) is an absolute requirement for the selection and development of B lymphocytes at multiple checkpoints. Binding to antigen via the BCR is complemented by a co-stimulus delivered through accessory and co-stimulatory cell surface molecules that regulate the signalling thre...
journal_title:Molecular immunology
pub_type:
doi:10.1016/s0161-5890(02)00203-1
更新日期:2003-01-01 00:00:00
abstract::In this study we demonstrate that histone deacetylase (HDAC)-inhibitor mediated cell surface expression of the structural different NKG2D-ligands MICA/B and ULBP2 is calcium-dependent. Treatment with the calcium chelator EGTA inhibited constitutive as well as HDAC-inhibitor induced MICA/B and ULBP2 cell surface expres...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.08.011
更新日期:2013-03-01 00:00:00
abstract::Scygonadin is a novel antimicrobial peptide, which was originally isolated from the seminal plasma of the mud crab, Scylla serrata. Based on the partial 20-residue NH(2)-terminal sequence of the peptide, H-Gly-Gln-Ala-Leu-Asn-Lys-Leu-Met-Pro-Lys-Ile-Val-Ser-Ala-Ile-Ile-Tyr-Met-Val-Gly-OH, scygonadin was cloned from th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.036
更新日期:2007-03-01 00:00:00
abstract::Malignant transformation of hepatocytes is frequently associated with upregulation of HLA-A expression. Currently there is no information available regarding the mechanisms underlying this phenotypic change. We investigated HLA-A expression in 165 paraffin embedded tissues and 21 fresh tissues from liver cancer patien...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.001
更新日期:2009-06-01 00:00:00
abstract::The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.07.010
更新日期:2010-11-01 00:00:00
abstract::Noxiustoxin (NTX) is a short-chain toxin from the venom of the scorpion Centruroides noxius Hoffmann, whose molecular structure and physiological effects have been characterized in detail, whereas the antigenic properties of this and other K(+) channel-blocking toxins are poorly studied. A monoclonal antibody against ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00091-2
更新日期:2000-08-01 00:00:00
abstract::Optineurin (Optn) is an adaptor protein with homology to NF-κB essential modulator (NEMO), the regulatory subunit of the IκB kinase (IKK) complex. Dysregulation of Optn has been linked to neurodegenerative, autoimmune and bone diseases. Optn shares a high degree of homology with NEMO, but is not part of the same high-...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.01.027
更新日期:2017-05-01 00:00:00
abstract::Genetic and functional studies suggest that polymorphism in cytotoxic T lymphocyte-associated antigen-4 (CTLA4) and inducible costimulator (ICOS) genes, both reported to harbour autoimmune susceptibility loci, could regulate the immune activation through affecting their expression and splicing of CTLA4. To address thi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.010
更新日期:2007-03-01 00:00:00
abstract::Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90086-7
更新日期:1988-01-01 00:00:00
abstract::The xenotransplantation research is driven by the shortage of allografts for transplantation of patients with end-stage organ failure and by lack of success in developing suitable artificial organs. Pigs are now generally accepted to be the donor species of choice, although a possible risk for transmission of xenozoon...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(99)00050-4
更新日期:1999-03-01 00:00:00