Abstract：
:Interleukin 10 (IL-10)-producing regulatory B-cells (Bregs) suppress inflammatory responses that mediate autoimmune diseases. However, it is unknown whether Bregs derive from a pre-existing dedicated B-cell lineage or if any B-cell can differentiate into Bregs in response to BCR or TLR activation. GL7(+) B-cells are antigen-experienced differentiated B-cells while GL7(-/lo) are at an early stage of B-cell differentiation. While both GL7(-/lo) and GL7(+) B cells can produce IL-10, differentiation of GL7(-) B-cells into Bregs does not require CD19- or Bcl6-induced signals, suggesting that BCR-induced proliferation or Ig class-switching is not necessary for generation of Breg cells. Of particular importance, we show that GL7(-) Breg cells are dramatically expanded in lupus-like mice and GL7(-) Bregs suppressed inflammatory responses in lupus-like mice by inducing expansion of Foxp3(+)Treg cells. Taken together, these results suggest that pre-existing GL7(-)IL-10(+) cells are expanded during inflammation, differentiate into GL7(+) Bregs and contribute to immune-regulation in lupus-like mice.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Wang X,Wei Y,Xiao H,Liu X,Zhang Y,Han G,Chen G,Hou C,Zhang L,Ma N,Shen B,Li Y,Egwuagu CE,Wang Rdoi
10.1016/j.molimm.2016.01.011subject
Has Abstractpub_date
2016-03-01 00:00:00pages
54-63eissn
0161-5890issn
1872-9142pii
S0161-5890(16)30012-8journal_volume
71pub_type
杂志文章abstract：:Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90030-2
更新日期：1994-10-01 00:00:00
abstract：:The p38 mitogen-activated protein kinases (MAPKs) are essential cytoplasmic signal molecules of innate immune pathways that play a vital role in host immune defense responses to pathogenic challenges. In this study, two fish p38 genes (Cip38α and Cip38β) were characterized for the first time from the grass carp Ctenop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.12.007
更新日期：2020-02-01 00:00:00
abstract：:TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.011
更新日期：2010-11-01 00:00:00
abstract：:Low molecular weight hemorrhagins were purified from crude Bothrops atrox snake venom by gel filtration followed by ionic strength chromatography. The protein fractions obtained, designated HI-1 to HI-8, contained proteins with molecular masses lower than 30 kDa. HI-5, the most representative among of these fractions,...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.11.032
更新日期：2004-03-01 00:00:00
abstract：:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90055-5
更新日期：1990-01-01 00:00:00
abstract：:Human complement components C6 and C7 have been purified and characterized. Component C6 is a single-chain plasma protein of mol. wt 104,800 containing 3.8% carbohydrate and it has alanine as the amino terminal residue. Component C7 is a single-chain plasma protein of mol. wt 92,400 containing 6.4% carbohydrate and it...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90189-4
更新日期：1982-11-01 00:00:00
abstract：:The allergenic cross-reactivity of cytochromes c isolated from Kentucky bluegrass (KBG) and ryegrass (RG) pollens was demonstrated by the finding that both cytochromes elicited PCA reactions of comparable titers in rats sensitized with murine reaginic antiserum to either KBG or RG cytochrome c. However, allergenic dif...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90263-2
更新日期：1982-12-01 00:00:00
abstract：:This study analyzes the gene repertoire coding for antibodies to an evolutionary novel immunogenic carbohydrate antigen in mice. The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) is an autoantigen, abundantly expressed in wild type mice, but absent in alpha 1,3galactosyltransferase knock-out (KO) mice, where i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00064-x
更新日期：2000-06-01 00:00:00
abstract：:SHP-1 plays an important role for the regulation of signaling from various hematopoietic cell receptors. In this study, we examined IL-3-induced cell proliferation and IL-3 depletion-induced apoptosis in bone marrow-derived mast cells (BMMC) established from motheaten (me) that lack SHP-1 expression, viable motheaten ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.10.001
更新日期：2011-01-01 00:00:00
abstract：:A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.07.282
更新日期：2007-02-01 00:00:00
abstract：:In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00079-0
更新日期：1998-10-01 00:00:00
abstract：:To investigate the IL-6/IL-6 receptor system, we obtained five murine monoclonal antibodies against human IL-6, which neutralize its biological activity. We classified them into two groups (Type I mAb and Type II mAb) according to the epitopes they recognized. These two types of antibodies showed no difference in the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90001-z
更新日期：1991-11-01 00:00:00
abstract：:Recombinant IL-2 (rIL-2) produced in prokaryotes, is widely used in lieu of native IL-2, which is secreted by T cells, to assess the functional profile of this cytokine. We provide evidence that a naturally occurring species of post-translationally modified IL-2 (moIL-2) exhibits enhanced bioactivity compared to rIL-2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00102-0
更新日期：2003-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) are now recognized as important regulators of all cellular processes, including immune function and cancer survival. These evolutionary preserved, single-stranded, non-coding RNA molecules mediate important functional effects primarily through post-transcriptional regulation of protein expression. M...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.07.022
更新日期：2019-11-01 00:00:00
abstract：AIM:To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient (CR1D) patient and healthy controls with low, medium and high CR1 numbers. METHODS:Alexa-labell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.014
更新日期：2019-10-01 00:00:00
abstract：:A computerized method for predicting the locations of protein antigenic determinants is presented, which requires only the amino acid sequence of a protein, and no other information. This procedure has been used to predict the major antigenic determinant of the hepatitis B surface antigen, as well as antigenic sites o...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90029-9
更新日期：1983-04-01 00:00:00
abstract：:The NLRP3 inflammasome constitutes a major antiviral host defense mechanism during influenza virus infection. Inflammasome assembly in virus-infected cells facilitates autocatalytic processing of pro-caspase-1 and subsequent cleavage and secretion of proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.023
更新日期：2017-06-01 00:00:00
abstract：:Triptolide is an active component of extracts derived from the medicinal vine Tripterygium Wilfordii Hook. f. (TWHF) whose extracts have been used to treat inflammatory bowel disease (IBD). We have reported that triptolide showed therapeutic activity in a murine IBD model, but the potential mechanism of action of this...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.06.007
更新日期：2010-09-01 00:00:00
abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90139-6
更新日期：1983-11-01 00:00:00
abstract：:Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.11.020
更新日期：2019-01-01 00:00:00
abstract：:Chicken and turkey beta 2-m were isolated from citrated plasma in sequential use of three chromatographic steps: affinity chromatography, gel filtration chromatography and anion-exchange chromatography. The purified protein was identified as beta 2-m by reaction with a beta 2-m specific monoclonal antibody and by the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90015-5
更新日期：1986-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The complement system plays an important role in the development of acute coronary syndrome (ACS). Complement C1q is an important initial component of the classical complement pathway and closely related to many chronic inflammatory diseases, including atherosclerosis (AS). We aimed to determi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.02.012
更新日期：2020-04-01 00:00:00
abstract：:Superantigens, like staphylococcal enterotoxin A (SEA), induce a strong proliferative response followed by clonal deletion of a substantial portion of defined V(beta) T-cells. The remaining cells display in vitro anergy. Anergy is a major mechanism to ensure antigen-specific tolerance in T-lymphocyte in the adult. Co-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.001
更新日期：2004-05-01 00:00:00
abstract：:CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.030
更新日期：2019-09-01 00:00:00
abstract：:The role of secondary structures and base mutability at different levels of transcription and supercoiling is analyzed in variable region antibody genes VH5, VH94 and VH186.2. The data are consistent with a model of somatic hypermutation in which increasing levels of transcription and secondary structure stability cor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.05.012
更新日期：2008-08-01 00:00:00
abstract：:Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.09.025
更新日期：2015-12-01 00:00:00
abstract：:Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.11.002
更新日期：2010-02-01 00:00:00
abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90216-4
更新日期：1982-03-01 00:00:00
abstract：:Host-derived macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infection, especially in malarial anemia. Although two Plasmodium parasite-derived MIF orthologs, Plasmodium falciparum MIF and P. berghei MIF were identified recently, the crystal structure and the precise rol...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.10.037
更新日期：2010-01-01 00:00:00
abstract：:The complete cDNA sequences of two bovine IgGs were obtained by RT-PCR cloning. The first-strand cDNA was prepared from an animal homozygous (A2/A2) for IgG2a; the resulting sequences of the two bovine IgGs reported here were identified as IgG2a(A2) and IgG1. These sequences, and their deduced amino acid sequences, ar...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00107-7
更新日期：1996-02-01 00:00:00
