Abstract：
:Chicken and turkey beta 2-m were isolated from citrated plasma in sequential use of three chromatographic steps: affinity chromatography, gel filtration chromatography and anion-exchange chromatography. The purified protein was identified as beta 2-m by reaction with a beta 2-m specific monoclonal antibody and by the ability to recombine with the chicken MHC class I heavy chain. The purity was estimated by SDS-PAGE and IEF. The pI was between 5.1 and 5.3 for chicken beta 2-m and 4.7 and 4.8 for turkey beta 2-m, which fact is reflected in their different electrophoretic mobilities in agarose gel (turkey migrates in the alpha and chicken migrates in the beta region). The mol. wt of both chicken and turkey beta 2-m was 14,500 estimated by SDS-PAGE whereas calculations based on the amino acid compositions gave mol. wts of 11,000. EM280 was 15.9 for chicken beta 2-m and 16.4 for turkey beta 2-m. The amino acid compositions and sequences of the two avian beta 2-m molecules have been compared with earlier data from the literature. The sequence of the 23 N-terminal amino acids was found to be identical in our preparations from both chicken and turkey, namely DLTPKVQVYSRFPASAGTKNVLN, and is incompatible with a previously published sequence also thought to be from turkey beta 2-m. Reasons for our opinion that the molecules isolated and sequenced in this paper are the correct ones are given.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Skjødt K,Welinder KG,Crone M,Verland S,Salomonsen J,Simonsen Mdoi
10.1016/0161-5890(86)90015-5subject
Has Abstractpub_date
1986-12-01 00:00:00pages
1301-9issue
12eissn
0161-5890issn
1872-9142journal_volume
23pub_type
杂志文章abstract：:The paper evaluates the role of the concept of degeneracy in current immunology. The historical background of the notion is considered, as well as that of related concepts like 'cross-reactivity' and 'promiscuity'. A distinction is made between degeneracy, as a property of a system and promiscuity, as a property of an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2003.11.021
更新日期：2004-02-01 00:00:00
abstract：:A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one N-linked glyco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.04.012
更新日期：2008-07-01 00:00:00
abstract：:This study describes serological and biochemical properties of a novel MHC class I molecule. The mutant H-2Ksm1 molecule was discovered in a mouse because of loss of reactivity of its peripheral blood lymphocytes to monoclonal antibodies. This mutation in the H-2Ks molecule is the first in vivo mutation described that...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90223-k
更新日期：1992-12-01 00:00:00
abstract：:This study aimed to co-culture Jurkat T lymphocytes with inactivated Mycobacterium tuberculosis (Mtb H37Ra), explore whether T lymphocytes could phagocytose H37Ra cells, and determine the underlying mechanism. Jurkat T lymphocytes were co-cultured with H37Ra cells, and confocal laser scanning microscopy, electron micr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.04.018
更新日期：2015-08-01 00:00:00
abstract：:In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.01.001
更新日期：2004-04-01 00:00:00
abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90094-4
更新日期：1984-01-01 00:00:00
abstract：:Activation of Jurkat T cells with phytohemagglutinin, CD3 or CD2 mAbs results in a marked inhibition of phosphatidylserine (PS) synthesis. Monitoring PS synthesis in T cells shows that: (i) after modulation of CD3 molecules the cells become refractory to further treatment with CD3 mAbs as well as to a further challeng...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90072-2
更新日期：1989-11-01 00:00:00
abstract：:Cleavage by the proteasome is responsible for generating the C terminus of T-cell epitopes. Modeling the process of proteasome cleavage as part of a multi-step algorithm for T-cell epitope prediction will reduce the number of non-binders and increase the overall accuracy of the predictive algorithm. Quantitative matri...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.12.013
更新日期：2006-05-01 00:00:00
abstract：:Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90105-7
更新日期：1992-02-01 00:00:00
abstract：:To clarify the different anti-CD26 mAbs corresponding different functions of CD26, the correlation of the epitopes defined by anti-CD26 mAbs and the functions of CD26 have been studied. Using truncated, human-rat CD26 swap mutants and cross-blocking studies, 13 anti-CD26 mAbs were divided into 5 separate groups. These...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00015-7
更新日期：1998-01-01 00:00:00
abstract：:C-reactive protein (CRP) is a pattern recognition molecule that binds several microbial and host ligands. Ligand-bound CRP activates the complement system via the classical pathway. Previously, we identified human complement factor H-related protein 4 (CFHR4), a member of the factor H protein family, as a CRP binding ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.005
更新日期：2010-03-01 00:00:00
abstract：:Mutations in the RAS family of oncogenes are highly prevalent in human cancer and, amongst its manifold effects, oncogenic RAS impairs the expression of components of the antigen presentation pathway. This allows evasion of cytotoxic T lymphocytes (CTL). CTL and natural killer (NK) cells are reciprocally regulated by ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.11.020
更新日期：2014-04-01 00:00:00
abstract：:Recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) is important in innate immune signaling. Toll-like receptors (TLRs) are well-characterized PRRs and are pivotal in antiviral and antitumor host defense. TIR domain-containing adaptor molecule 1 (TICAM-1, also called T...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.03.010
更新日期：2016-05-01 00:00:00
abstract：:The specificities of the combining sites of 19 mouse monoclonal antibodies to dextran B1355S have been characterized immunochemically by quantitative precipitin and precipitin inhibition assays; association constants for B1355S were determined by affinity gel electrophoresis. Cross-reactive and individual idiotypes re...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90139-2
更新日期：1986-04-01 00:00:00
abstract：:A panel of human T cell clones specific for measles virus was characterized and among them fusion protein-specific, DR1-and DP-restricted T cell clones were selected to study the processing and presentation of determinants borne by a viral membrane protein. Using two independent methods to assess the activation of T c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00148-4
更新日期：1996-03-01 00:00:00
abstract：:Bothropasin is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca venom, the snake responsible for most bites in Southeastern Brazil. SVMPs, such as bothropasin, are involved in the main bothropic envenoming symptoms, which include hemorrhage, inflammation, necrosis and blood coagulation deficie...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.10.019
更新日期：2018-12-01 00:00:00
abstract：:Mammalian cells produce inflammatory cytokines and chemokines in response to innate immune signals and their expression is tightly regulated. Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is an inflammatory chemokine belonging to the CXC chemokine family....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.11.007
更新日期：2017-01-01 00:00:00
abstract：:An antigen-coated plate radioimmunoassay was used to detect antibodies against homologous glutaraldehyde-modified albumin (in monomeric or polymeric form) in normal mouse sera. Mouse antibodies reacted also with heterologous glutaraldehyde-treated albumin; for instance human albumin. Immunoelectrophoresis of purified ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90310-8
更新日期：1982-08-01 00:00:00
abstract：:ANCA associated vasculitis is a serious, very often recurrent disease that despite the current standard treatment with high-dose glucocorticoids and either cyclophosphamide or rituximab, patients have a nine-fold increased mortality risk in the first year compared with healthy controls, attributed to infections, vascu...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2019.06.018
更新日期：2019-08-01 00:00:00
abstract：:alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90079-5
更新日期：1989-03-01 00:00:00
abstract：:The protective antigen (PA(83)) of Bacillus anthracis is the dominant antigen in natural and vaccine-induced immunity to anthrax infection. Three human single-chain variable fragments (scFvs) against cell bound PA were isolated from an antibody phage display library. Specifically, the antibodies were evaluated for the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.11.011
更新日期：2007-04-01 00:00:00
abstract：:The kinetics of the cryoprecipitation of two monoclonal IgG and two monoclonal IgM cryoimmunoglobulins, two IgM/IgG mixed cryoglobulins and a series of cold soluble monoclonal IgG and IgM immunoglobulins in the presence of polyethylene glycol have been compared by time dependent turbidity measurements. The effects of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90001-0
更新日期：1987-09-01 00:00:00
abstract：:Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of TBK1 and interferon pathway and the expression of SOCS3 is closely correlated with symptoms of influenza patients. However, whether deletion of Socs3 in the lung epithelial cells would affect influenza lung replication and inflammation in vivo is un...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.022
更新日期：2020-09-01 00:00:00
abstract：:A monoclonal antibody (vpg15) has been described which can block infection with feline immunodeficiency virus (FIV) and which recognizes the feline homologue of CD9. In order to study the role of feline CD9 in infection with FIV we have molecularly cloned a cDNA encoding feline CD9 by R.A.C.E (rapid amplification of c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00008-3
更新日期：1995-04-01 00:00:00
abstract：:N-substituted benzamides are compounds that have recently been reported to inhibit nuclear factor-kappaB (NF-kappaB) activity and induce apoptosis in a pre-B cell line. In this study, we focused on the effects of N-substituted benzamides on transcriptional regulation in Jurkat T cells. We used a model system where the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00060-8
更新日期：2001-08-01 00:00:00
abstract：:Dendritic cells (DC) are comprised of several subsets with distinct functions, differing in their capacity to respond to pathogen products. To gain novel insights into their pathogen specificity, we compared the protein composition of the plasma membrane of CD8+ and CD8- DC, directly isolated from mouse spleens. Diffe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.028
更新日期：2010-05-01 00:00:00
abstract：:The complement system is very tightly regulated by fluid-phase and membrane-bound factors that prevent injury to self-tissues. The study of genetically engineered animals with targeted deletion or gain of function mutations has highlighted the important role that many of the complement inhibitors play in vivo. The adv...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(03)00110-x
更新日期：2003-09-01 00:00:00
abstract：:Sepsis is a life-threatening disease characterized by uncontrolled inflammatory responses upon pathogen infections, especially for the antibiotic-resistant strains, such as Methicillin-resistant S. aureus (MRSA). Here we demonstrated that a Mitochondria-derived peptide (MOTS-c) could significantly improve the survival...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.10.017
更新日期：2017-12-01 00:00:00
abstract：:Conjugates of monoclonal antibodies and Pseudomonas exotoxin A (PE) were formed with disulfide or thioether bonds. Thioether conjugates which formed with succinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (SMCC) modified PE and reduced antibody formed with an 80% yield of equimolar conjugate within 30 min wi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90140-u
更新日期：1990-03-01 00:00:00
abstract：:In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00079-0
更新日期：1998-10-01 00:00:00
