Abstract:
:Triptolide is an active component of extracts derived from the medicinal vine Tripterygium Wilfordii Hook. f. (TWHF) whose extracts have been used to treat inflammatory bowel disease (IBD). We have reported that triptolide showed therapeutic activity in a murine IBD model, but the potential mechanism of action of this agent in IBD remains elusive. Accumulated data showed that both T-helper (Th) 1 and Th17 response may contribute to pathogenesis of human IBD and animal colitis. Interleukin (IL)-6/signal transducer and activator of transcription-3 (STAT3) signaling pathway play an important role in Th17 response as well as pathophysiology of IBD. We hypothesized that triptolide would attenuate the experimental colitis by repressing IL-17 and that this would involve down-regulation of IL-6/STAT3 signaling pathway. Histological examination demonstrated that triptolide significantly reduced the severity of colitis in C3H/HeJBir.IL-10-deficeint mice. Triptolide suppressed the IL-6/STAT3 signaling pathway, as well as repressed gene expression of IL-17 in vivo. In addition, triptolide (20ng/ml) in vitro was able to down-regulate the IL-6/STAT3 pathway and reduce IL-17 expression in cultured colonic explants from patients with Crohn's disease (CD).
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Li Y,Yu C,Zhu WM,Xie Y,Qi X,Li N,Li JSdoi
10.1016/j.molimm.2010.06.007subject
Has Abstractpub_date
2010-09-01 00:00:00pages
2467-74issue
15eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00476-1journal_volume
47pub_type
杂志文章abstract::Clonal idiotypic immunoglobulins of follicular lymphomas can be isolated by somatic fusion procedures. Idiotypic IgMs (Id-IgM) were isolated from two patients and used to immunise a strain of mice, deficient in mouse antibody production and engineered with yeast artificial chromosomes (YAC) containing fragments of the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.03.035
更新日期:2004-07-01 00:00:00
abstract::Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90089-7
更新日期:1987-02-01 00:00:00
abstract::Many T cell responses are dominated by restricted TCR expression and can range from repeated usage of particular TCR Vbeta- and/or Valpha-elements, to the preferential usage of both V- and J-elements, often in conjunction with conserved V-D-J or V-J junctional sequences. Cytotoxic T lymphocytes specific for a Kb-restr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00051-0
更新日期:1998-04-01 00:00:00
abstract::Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.018
更新日期:2010-04-01 00:00:00
abstract::We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signal...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.02.001
更新日期:2017-05-01 00:00:00
abstract::We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.10.005
更新日期:2014-02-01 00:00:00
abstract::Prolactin has several immune functions in fish however, the effects on innate and specific components of rainbow trout immunity are currently unknown. Therefore in this study, prolactin peptide (pPRL) injection in rainbow trout generated anti-PRL antibodies that were confirmed through Western blot assays of fish brain...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.020
更新日期:2020-11-01 00:00:00
abstract::Two monoclonal antibodies, HH8 and HH9, have been established after immunization of mice with galactosyl-A glycolipid antigen having the terminal structure, Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----R, which is the precursor for type 3 chain A (repetitive A) and type 3 chain H (A-associated H)....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90068-5
更新日期:1988-02-01 00:00:00
abstract::T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.002
更新日期:2020-11-01 00:00:00
abstract::GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the mechanisms underlying the activation of these antigen-presenting cells (APCs) by GK-1. In ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.09.015
更新日期:2020-12-01 00:00:00
abstract::The cell-surface receptor ST2L triggers cytokine release by immune cells upon exposure to its ligand IL-33. To study the effect of ST2L-dependent signaling in different cell types, we generated antagonist antibodies that bind different receptor domains. We sought to characterize their activities in vitro using both tr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.05.019
更新日期:2016-07-01 00:00:00
abstract::Sepsis-induced inflammatory damage is a crucial cause of acute kidney injury (AKI), and AKI is an ecumenical fearful complication in approximately half of patients with sepsis. CCAAT/enhancer-binding protein β (C/EBPβ) plays roles in regulating acute phase responses and inflammation. However, the role and mechanism of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.023
更新日期:2020-11-01 00:00:00
abstract::Ligation of the B cell Ag receptor (BCR) activates a protein-tyrosine kinase (PTK) and CD45 protein-tyrosine phosphatase (PTPase)-dependent signaling cascade that results in the activation of Ras. This pathway of Ras activation can operate independently of protein kinase C (PKC) activity. Activation of Ras may lead to...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00134-4
更新日期:1996-02-01 00:00:00
abstract::We have examined the regulation of the AP-1 DNA transcription complex during T cell activation in response to interleukin 1 (IL-1) and phorbol ester (TPA) treatment of the IL-1 responsive murine thymoma T cell line, EL4 6.1 C 10. IL-1 synergistically enhances the stimulatory effect of TPA on AP-1-mediated gene express...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00055-j
更新日期:1995-08-01 00:00:00
abstract::Renal transplantation is the treatment of choice for patients with end-stage renal disease. The vital role of the complement system in renal transplantation is widely recognized. This review discusses the role of complement in the different phases of renal transplantation: in the donor, during preservation, in reperfu...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.05.014
更新日期:2017-09-01 00:00:00
abstract::NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.007
更新日期:2008-03-01 00:00:00
abstract::Subunits of the mitochondrial ATP synthase complex are expressed on the surface of tumors, bind the TCR of human Vgamma9/Vdelta2 lymphocytes and promote their cytotoxicity. Present experiments show that detection of the complex (called ecto-F1-ATPase) at the cell surface by immunofluorescence correlates with low MHC-c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.05.026
更新日期:2008-01-01 00:00:00
abstract::Mouse monoclonal antibodies (MAbs) have been prepared against rat T cell blasts. One MAb called MRC OX-40 recognized an antigen that differed from any previously described in that its expression was detected only on T blasts that also expressed the CD4 antigen. The OX-40 MAb did not detect an activation determinant of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90122-2
更新日期:1987-12-01 00:00:00
abstract::Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90086-7
更新日期:1988-01-01 00:00:00
abstract::Immune-mediated glomerular diseases (glomerulonephritis) encompass a heterogeneous collection of diseases that cause inflammation within the glomerulus and other renal compartments with significant morbidity and mortality. In general, CD4+ T cells orchestrate the immune response and play a unique role in autoimmune an...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.09.005
更新日期:2018-12-01 00:00:00
abstract::The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation ha...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.01.018
更新日期:2019-03-01 00:00:00
abstract::DC-SIGN (dendritic cell-specific ICAM-3-grabbing non-integrin) is a myeloid pathogen-attachment factor C-type lectin which recognizes mannose- and fucose-containing oligosaccharide ligands on clinically relevant pathogens. Intracellular signaling initiated upon ligand engagement of DC-SIGN interferes with TLR-initiate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.036
更新日期:2010-01-01 00:00:00
abstract::Polymorphonuclear cells (PMN) are widely recognized as sophisticated killers during microbial infections. In recent years, PMN have been shown to interact and functionally interfere with other cells of the immune system. In this study, we investigated PMN-T cell interactions in an in vitro co-culture system. A relativ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.07.006
更新日期:2011-09-01 00:00:00
abstract::Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90169-4
更新日期:1983-12-01 00:00:00
abstract::Protein kinases have been implicated in a number of regulatory mechanisms including signal transduction in many cells. To address the possibility that the large granular lymphocyte (LGL) also uses one or more unique protein kinases for LGL functions, an efficient method was developed to obtain partial cDNA clones for ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90153-b
更新日期:1991-04-01 00:00:00
abstract::Stimulation of cells of the rat basophilic leukemia line RBL-2H3, which are used as a model in biochemical studies of mast cells, by antigen or by the calcium ionophore ionomycin, are known to cause secretion of mediators of inflammation. These stimuli have now been found to cause a decrease in the cells' cytosolic pH...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90142-3
更新日期:1988-11-01 00:00:00
abstract::The kinetics of the cryoprecipitation of two monoclonal IgG and two monoclonal IgM cryoimmunoglobulins, two IgM/IgG mixed cryoglobulins and a series of cold soluble monoclonal IgG and IgM immunoglobulins in the presence of polyethylene glycol have been compared by time dependent turbidity measurements. The effects of ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90001-0
更新日期:1987-09-01 00:00:00
abstract::Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90195-x
更新日期:1982-11-01 00:00:00
abstract::The carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus) was investigated by quantitative precipitin analysis using blood group A, B, H, Le and I substances and by precipitation inhibition with various mono- and oligosaccharides. The lectin precipitated best with A1 subst...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90101-6
更新日期:1989-02-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium (S. Typhimurium) changes the structure of its lipopolysaccharide (LPS) in response to the environment. The two main LPS variants found in S. Typhimurium correspond to LPS with a hepta-acylated lipid A (LPS 430) and LPS with modified phosphate groups on its lipid A (LPS 435). We ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.03.003
更新日期:2019-07-01 00:00:00