Abstract：
:We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signals on PCV2b infected swine lymphocytes by indirect immunofluorescence staining. The mAb 1H3 bound to three minimal linear epitopes (P62, DPPLNP; P67, DPPLNPK; P73, LKDPPLKP), which was located at Cterminus of the capsid protein of PCV2b-1A/1B, PCV2b-1C, and PCV2a-2A respectively. The mAbs 3B2 bound to only one minimal linear epitopes (P59, KDPPLNP). The mAbs 6B8 bound to two minimal linear epitopes (P59 and P67). Our data demonstrate the core motif (P62) within the P59 could be recognized by mAbs (3B2 and 6B8) in the free status by liquid phase blocking immunoassay (LPBI) but not be recognized by these mAbs in the fixed form on the plate by indirect ELISA (iELISA). However, the P73 could be recognized by mAb 1H3 by iELISA but no inhibition of the interactive binding of C3 and mAb 1H3 by LPBI. This study also indicated that IgM mAbs and defective Ig mAb have broad binding, moderate specificity and low affinity. This study confirm that mAbs have pluripotency of binding. It might be a phenomenon of antibody response to Cterminus of capsid protein of PCV2b.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Hung LC,Cheng ICdoi
10.1016/j.molimm.2017.02.001subject
Has Abstractpub_date
2017-05-01 00:00:00pages
100-110eissn
0161-5890issn
1872-9142pii
S0161-5890(17)30035-4journal_volume
85pub_type
杂志文章abstract：:We have previously shown that immunization with SIV-, SHIV-, or HA (influenza hemagglutinin)-virus-like particles (VLPs) elicits a strong humoral immune response in mice. However, little is known about the action VLPs exert on immune effector cells, including B cells. In this study, we found that all three types of VL...
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.010
更新日期：2010-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.031
更新日期：2008-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2003-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2017-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90113-0
更新日期：1985-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2014-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-07-01 00:00:00
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更新日期：2015-03-01 00:00:00
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更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
