Abstract:
:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by intradermal injection of 0.1 ml complete Freund's adjuvant. Synovium APRIL expression was detected by immunohistochemical analysis. The level of APRIL and matrix metalloproteinase (MMP)-9 were assayed by enzyme-linked immunosorbent assay. The expression of APRIL and its receptors (TACI, BCMA and BAFF-R) were assessed by immunofluorescence staining, flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR. The effects of APRIL on the function of FLS were investigated by MTT, Quantibody Rat Inflammation Array 1 and transwell assays, respectively. A higher concentration of APRIL was detected in AA synovium homogenate compared with normal group. AA FLS expressed APRIL, TACI, BAFF-R and BCMA at the mRNA levels, whereas only APRIL and BCMA were confirmed to be expressed on membrane by flow cytometry. APRIL stimulated AA FLS proliferation, migration and invasion and the secretion of proinflammatory factors. In addition, FLS cocultured with APRIL-treated B cells or T cells had a significantly greater proliferation than FLS cultured alone. Neutralization of APRIL by the TACI-Ig fusion protein attenuated these stimulating effects of APRIL on FLS. Our data indicate that APRIL may act as an important mediator for facilitating the function of FLS. Blockade of APRIL thus may be a valuable adjunct in the treatment of RA.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Chang Y,Jia X,Sun X,Xu S,Wu Y,Zhang L,Wei Wdoi
10.1016/j.molimm.2014.11.002subject
Has Abstractpub_date
2015-03-01 00:00:00pages
90-8issue
1eissn
0161-5890issn
1872-9142pii
S0161-5890(14)00294-6journal_volume
64pub_type
杂志文章abstract::Keratin is a cytoskeletal protein that constitutes the intermediate filament. Its distribution is restricted to epithelial tissues in mammals, but is wider in fish. An interesting feature of fish keratin is that it is abundant in the cutaneous mucus. However, the biological function of keratin in the mucus has not bee...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.09.012
更新日期:2019-12-01 00:00:00
abstract::Mammalian cells produce inflammatory cytokines and chemokines in response to innate immune signals and their expression is tightly regulated. Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is an inflammatory chemokine belonging to the CXC chemokine family....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.007
更新日期:2017-01-01 00:00:00
abstract::A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal p...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90123-9
更新日期:1986-09-01 00:00:00
abstract::The binding specificity of rat alveolar macrophages (AM phi) for sheep erythrocytes (E) coated with gangliosides GM1, GM2, GM3, GD1a, GD1b or GT1b was analyzed in a rosette assay by studying the inhibitory effect of gangliosides, various carbohydrates, IgG, C3b-like C3, and fibronectin in this assay. The uptake of gan...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90163-x
更新日期:1986-11-01 00:00:00
abstract::Individually amplified kappa cDNA molecules from single B lymphocytes revealed sequence heterogeneity and aberrantly spliced products. The nature and frequency of the base changes and their absence from similarly amplified beta2 microglobulin transcripts indicate that they were not derived by Taq polymerase misincorpo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00055-9
更新日期:2000-04-01 00:00:00
abstract::alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90079-5
更新日期:1989-03-01 00:00:00
abstract::Type III interferons (IFN-λs) comprise a group of newly identified antiviral cytokines that are functionally similar to type I IFNs and elicit first-line antiviral responses. Recently, type III IFNs were identified in several species; however, little information is available about type III IFNs in ducks. We compared t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.10.013
更新日期:2015-12-01 00:00:00
abstract::MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracell...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.09.004
更新日期:2016-10-01 00:00:00
abstract::The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magno...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.12.004
更新日期:2007-04-01 00:00:00
abstract::Binding of the catecholamine beta-adrenergic antagonist, l-alprenolol, by the IgGl anti-alprenolol monoclonal antibody 37A4 was examined using the radioligand 3H-dihydroalprenolol as an extrinsic signal and the increase in antibody fluorescence upon l-alprenolol binding as intrinsic signal. Equilibrium binding studies...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90043-5
更新日期:1987-06-01 00:00:00
abstract::The isolation and characterization of two human serum proteins, RHP and N-RHP, are described. N-RHP appears to be the normal counterpart of RHP which is found at elevated levels in sera of patients with rheumatoid arthritis [Rosano et al. (1988b) Inflammation 12, 351 - 360]. Although both proteins crossreact with anti...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00068-2
更新日期:1995-11-01 00:00:00
abstract::Phosphocholine (PC)-specific antibodies that arise in the mouse in response to Proteus morganii (PM) and use V1-DFL16.1-JH1 are characterized by a number of recurring mutations. Most striking is an invariant A for G substitution in codon 95 of VH which results in an asparagine instead of aspartate at that position. Be...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90069-n
更新日期:1993-03-01 00:00:00
abstract::Pathogenic micro-organisms employ a broad range of strategies to survive in and to persistently infect the human host. Far from being completely understood by which highly sophisticated means invading pathogens overcome the host's destructive immune defence, there is a growing body of evidence on particular mechanisms...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2005.06.016
更新日期:2006-01-01 00:00:00
abstract::Dendritic cells (DCs) are the professional antigen-presenting cells (APC) which efficiently prime the immune response or induce tolerance. We recently identified Dendritic Cell Specific TrAnsMembrane Protein (DC-STAMP), a novel 470 amino acid protein preferentially expressed by dendritic cells. Previously we demonstra...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.04.019
更新日期:2010-07-01 00:00:00
abstract::Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was coll...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.007
更新日期:2006-04-01 00:00:00
abstract::REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversifica...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.09.017
更新日期:2006-04-01 00:00:00
abstract::A Brucella abortus protein with a molecular weight of 50 kDa has been shown to bind bovine immunoglobulin G from healthy, brucellosis-free animals. The Brucella immunoglobulin G binding molecule appears to be a protein, since it is susceptible to proteolysis. The protein is presumed to be located on the cell surface, ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90084-w
更新日期:1991-01-01 00:00:00
abstract::Complement plays a dual role in the progression of systemic lupus erythematosus since it has important protective functions, such as the clearance of immune complexes and apoptotic cells, but is also a mediator of renal inflammation. To investigate this balance in a clinically relevant setting, we investigated how tar...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.09.015
更新日期:2011-10-01 00:00:00
abstract::The testicular cystic structure and the abrupt morphological changes that the fish testis undergoes during the reproductive cycle (RC) make it an interesting model for studying the regulation of spermatogenesis, in particular the role of matrix metalloproteinases (Mmps). The gilthead seabream is a seasonal breeding te...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.031
更新日期:2008-05-01 00:00:00
abstract::Hyperimmune absorbed rabbit antisera which were reactive with epitopes specific for individual variants of human placental alkaline phosphatase were tested for their reactivity with primate placental alkaline phosphatases. Using the three epitope-specific reactivities defined previously, we found that: epitope I is pr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90074-9
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND:Complement component C5-derived C5a locally generated in the brain has been shown to protect against glutamate-induced neuronal apoptosis and beta-amyloid (Aβ) toxicity, but the mechanism is not clear. In this study, we tested the hypothesis that C5a influences upstream signal transduction pathways associate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.06.016
更新日期:2013-12-01 00:00:00
abstract::Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.12.004
更新日期:2010-03-01 00:00:00
abstract::The mechanisms of each subset of immune cells contributing to the pathogenesis of viral hepatitis remain incompletely understood. In this study, we examined the role of liver CD4(-) CD8(-) (double negative, DN) T cells during murine hepatitis virus strain 3 (MHV-3)-induced hepatitis in C3H/HeJ mice. We demonstrate tha...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.05.017
更新日期:2012-10-01 00:00:00
abstract::An antigen-coated plate radioimmunoassay was used to detect antibodies against homologous glutaraldehyde-modified albumin (in monomeric or polymeric form) in normal mouse sera. Mouse antibodies reacted also with heterologous glutaraldehyde-treated albumin; for instance human albumin. Immunoelectrophoresis of purified ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90310-8
更新日期:1982-08-01 00:00:00
abstract::Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.025
更新日期:2007-03-01 00:00:00
abstract::In immune cells, Src-family kinases have been implicated as critical regulators of a large number of intracellular signaling pathways. Studies of gene knockout mice lacking various family members have both confirmed the requirement for these kinases in some pathways and provided surprising insight into their roles in ...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.04.010
更新日期:2004-07-01 00:00:00
abstract::Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90169-6
更新日期:1987-11-01 00:00:00
abstract::Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00032-4
更新日期:2003-06-01 00:00:00
abstract::Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.028
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND:Bladder cancer (BC) can be successfully treated by manipulating immune responses with intravesical Bacillus Calmette-Guerin instillation or targeting the PD-1/PD-L signaling pathway. In the present study we investigated the prognostic significance of the immune checkpoint inhibitor PD-1 and its ligands PD-L1...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.05.010
更新日期:2020-08-01 00:00:00