Abstract：
:Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was collected from fish 6, 12, 24 and 48 h following infection or at the same time points from fish injected with PBS as non-infected control. PCR-select cDNA subtraction libraries were constructed from pooled 24 and 48 h post infection RNA to identify up-regulated mRNAs. One thousand four hundred and eighty six cDNA clones were sequenced from enriched cDNA libraries, of which 71% had significant homologies to known functional proteins. Many of these clones have previously been un-characterised in Atlantic salmon. A salmonid cDNA microarray was used to further analyse the gene expression profile as the library construction in itself does not answer the dynamics of the response. The greatest increase in expression identified in the array analysis was a liver antibacterial peptide, hepcidin that was increased 11-fold following the challenge. A panel of clones were chosen for semiquantitative reverse transcriptase PCR from all time points sampled. These results indicated there were both temporal differences and tissue differences in the transcriptional response to bacterial exposure, potentially of relevance to the establishment of protection.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Martin SA,Blaney SC,Houlihan DF,Secombes CJdoi
10.1016/j.molimm.2005.10.007subject
Has Abstractpub_date
2006-04-01 00:00:00pages
1900-11issue
11eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00366-4journal_volume
43pub_type
杂志文章abstract：:Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.04.009
更新日期：2016-06-01 00:00:00
abstract：:Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates hematopoiesis, inflammation, immune responses and bone homeostasis in mammals. Fish IL-6 has been cloned in recent years but to date no functional studies have been reported. Thus, in this paper we present for the first time in fish the functional character...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.05.027
更新日期：2011-09-01 00:00:00
abstract：:Macrophages play an important role both in innate and adaptive immune responses. Treatment with interferon (IFN) γ together with lipopolysaccharide (LPS) activates pro-inflammatory macrophages which secrete various pro-inflammatory cytokines including IL-12. IL-12 promotes a Th1 type immune response by directly contro...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.03.017
更新日期：2012-07-01 00:00:00
abstract：:A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90159-9
更新日期：1988-11-01 00:00:00
abstract：:Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was u...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.08.015
更新日期：2014-02-01 00:00:00
abstract：:The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.001
更新日期：2007-02-01 00:00:00
abstract：:Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.030
更新日期：2009-06-01 00:00:00
abstract：:Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90170-5
更新日期：1990-05-01 00:00:00
abstract：:In addition to the antigen receptor, resting T cells express a number of receptors that can be stimulated to generate proliferative signals. These "accessory" receptors require co-expression of the T cell receptor (TCR), suggesting that they channel their signals via secondary activation of the signal transduction fun...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00160-3
更新日期：1995-04-01 00:00:00
abstract：:A computerized method for predicting the locations of protein antigenic determinants is presented, which requires only the amino acid sequence of a protein, and no other information. This procedure has been used to predict the major antigenic determinant of the hepatitis B surface antigen, as well as antigenic sites o...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90029-9
更新日期：1983-04-01 00:00:00
abstract：:The peptide editor HLA-DM (DM) catalyzes the exchange of peptides bound to MHC class II molecules within antigen presenting cells by generating a "peptide-receptive" MHC class II conformation (MHC(receptive)) to which peptides readily bind and rapidly unbind. While recent work has uncovered the determinants of DM reco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.001
更新日期：2009-09-01 00:00:00
abstract：:Monoclonal antibodies were prepared against synthetic peptides synthetized on the basis of nucleotide sequence from cDNA and genomic clones encoding a class I antigen expressed by the rabbit RL-5 cell line. Using a peptide corresponding to positions 61-73 of the N domain, we were able to obtain an hybridoma producing ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90019-8
更新日期：1987-05-01 00:00:00
abstract：:Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.025
更新日期：2007-03-01 00:00:00
abstract：:T-cell receptor (TCR) repertoire diversity, thymic output, clonal size and peripheral T-lymphocyte numbers largely depend on intra-thymic and post-thymic T-lymphocyte proliferation. However, quantitative insight into thymocyte and T-lymphocyte proliferation is still lacking. We developed a new TCRG-based TCR excision ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.08.019
更新日期：2013-03-01 00:00:00
abstract：:The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magno...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.12.004
更新日期：2007-04-01 00:00:00
abstract：:The potency of a T cell is determined in large part by two interactions, binding of a cognate peptide to the MHC, and binding of the T cell receptor (TCR) to this pepMHC. Various studies have attempted to assess the relative importance of these interactions, and to correlate the corresponding binding parameters with t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.06.012
更新日期：2009-09-01 00:00:00
abstract：:Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.07.016
更新日期：2014-01-01 00:00:00
abstract：:The cell surface co-stimulatory protein CD154 (CD40L) is a target for monoclonal antibody (mAb) inhibitors of T-cell mediated immune diseases. This protein, like most other members of the TNF ligand family, forms homotrimeric complexes on the cell surface and in solution, with a three-fold axis of symmetry. We find th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00045-7
更新日期：2002-09-01 00:00:00
abstract：:An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90052-5
更新日期：1985-12-01 00:00:00
abstract：:Mycobacterium leprae, the causative agent of leprosy, challenges host defense mechanism by impairing the signal transduction of T cells which leads to downregulation of T cell proliferation, mainly as a consequence of interference with IL-2 production. In this study we sought to identify how soluble forms of M. leprae...
journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.molimm.2006.09.008
更新日期：2007-03-01 00:00:00
abstract：:Attempts to develop peptide vaccines, based on a limited number of peptides face two problems: HLA polymorphism and the high mutation rate of viral epitopes. We have developed a new genomic method that ensures maximal coverage and thus maximal applicability of the peptide vaccine. The same method also promises a large...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.003
更新日期：2007-02-01 00:00:00
abstract：:Mangiferin is the major bioactive ingredient in the leaves of Mangifera indica L., Aqueous extract of such leaves have been traditionally used as an indigenous remedy for respiratory diseases including cough and asthma in Traditional Chinese Medicine. Mangiferin was shown to exert its anti-asthmatic effect by modulati...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.025
更新日期：2019-10-01 00:00:00
abstract：:Osteopontin (OPN) is a cytokine with multiple functions, including the regulation of innate immune response. However, the detailed function and mechanism of OPN in host defense against invaded microorganisms remain unclear. In this report, we revealed that OPN could affect the clearance of Propionibacterium acnes in k...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.435
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Smalotrophomonas maltophilia(S. maltophilia) is common in nosocomial infections. However, few studies have revealed the effect of S. maltophilia on cellular immunity in the host's immune system up to now. In clinical work, we accidentally discovered that S. maltophilia directly stimulated T cells to secrete ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.12.019
更新日期：2021-02-01 00:00:00
abstract：:Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further charact...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90035-7
更新日期：1989-08-01 00:00:00
abstract：:The ability of bovine IgG preparations to bind to the various distinct human leukocyte Fc gamma receptors was studied. In experiments using intact cells and isolated Fc gamma receptors, it was demonstrated that bovine IgG can bind to Fc gamma receptors of four human cell types but not to Fc gamma receptors of human ne...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90144-1
更新日期：1987-03-01 00:00:00
abstract：:To clarify the different anti-CD26 mAbs corresponding different functions of CD26, the correlation of the epitopes defined by anti-CD26 mAbs and the functions of CD26 have been studied. Using truncated, human-rat CD26 swap mutants and cross-blocking studies, 13 anti-CD26 mAbs were divided into 5 separate groups. These...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00015-7
更新日期：1998-01-01 00:00:00
abstract：:It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.009
更新日期：2010-11-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.09.004
更新日期：2016-10-01 00:00:00
abstract：:Human promyelocytic leukemia (HL-60) cells were induced by 1,25-dihydroxyvitamin D3 (calcitriol) to differentiate and examined using a panel of monoclonal antibodies (MoAbs) and functional assays. Although morphologically and histochemically these cells appeared to be of the monocyte-macrophage phenotype, there was a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90180-4
更新日期：1985-05-01 00:00:00
