Abstract:
:Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further characterized. Both reacted strongly with mouse and calf thymus histones H1, but showed limited (if any) binding to H1 from non-mammalian species such as duck, trout, or sea urchin sperm. Histone H1 is composed of three domains: N (N-terminal), G (globular) and C (C-terminal). The binding of MRA3 and MRA12 antibodies to the N, G and C domains of the histone H1 molecule was also investigated, using purified H1 fragments. Significant binding was observed only with the GC fragment but not with isolated NG, G or C fragments. Moreover, the integrity of most, if not all, of the G domain (residues 33-122) was necessary for antibody binding, since cleavage of the H1 molecule either at residue 72 or 106 abolished the binding to MRA3 and MRA12. Taken together, these results could indicate that MRA3 and MRA12 antibodies recognize a conformational determinant of the H1 molecule.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Monestier M,Fasy TM,Böhm Ldoi
10.1016/0161-5890(89)90035-7subject
Has Abstractpub_date
1989-08-01 00:00:00pages
749-58issue
8eissn
0161-5890issn
1872-9142journal_volume
26pub_type
杂志文章abstract::alpha 1-Microglobulin (alpha lm), a glycoprotein heterogeneous in charge, was reported to occur both as a 31-kilodalton (kd) monomer [low mol. wt alpha lm (LMW-alpha lm)] and as polymers or complexes formed with other plasma proteins including IgA [high mol. wt alpha lm (HMW-alpha lm)]. The present study was designed ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90096-3
更新日期:1985-06-01 00:00:00
abstract::Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.09.025
更新日期:2015-12-01 00:00:00
abstract::CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.005
更新日期:2006-04-01 00:00:00
abstract::Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.02.024
更新日期:2007-01-01 00:00:00
abstract::Human endothelial cells have been shown to be distinctive from other IL-1 responsive cells (e.g. murine thymocytes), in the equal units of murine and human IL-1 do not have the same endothelial cell stimulatory effect, as measured by increased lymphocyte adherence. In this paper, the cell surface IL-1 receptors of hum...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90078-3
更新日期:1989-03-01 00:00:00
abstract::Human beta defensin-1 (hBD1) is a component of the immune system which links the innate and adaptive immune responses. We have demonstrated that hBD1 induces rapid cytolysis of prostate cancer cells and that it may also possess tumor suppressive abilities. In addition, there is a high frequency of cancer-specific loss...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.11.004
更新日期:2009-03-01 00:00:00
abstract::Sialic acid - binding immunoglobulin - like lectins (Siglecs) are members of the largest superfamily of immune receptors; they recognize sialic acid and are mainly expressed in immune cells. Studies on mammals indicate that Streptococcus agalactiae (GBS) evade immune reactions by interacting with the host immune cells...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.01.005
更新日期:2016-05-01 00:00:00
abstract::In addition to classic Smad signaling pathways, the pleiotropic immunoregulatory cytokine TGF-beta1 can activate MAP kinases, but a role for TGF-beta1-MAP kinase pathways in T cells has not been defined heretofore. We have shown previously that TGF-beta1 inhibits Th1 development by inhibiting IFN-gamma's induction of ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.024
更新日期:2007-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.molimm.2015.06.003
更新日期:2015-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00051-0
更新日期:1998-04-01 00:00:00
abstract::The alternative pathway of complement is receiving increasing attention as a therapeutic target because of recent findings in several animal models that support its essential role in tissue injury and disease pathogenesis. Although the contribution of alternative pathway activation to serum complement activation in vi...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.03.012
更新日期:2004-06-01 00:00:00
abstract::Tumors exploit immunoregulatory checkpoints that serve to attenuate T cell responses as a means of circumventing immunologic rejection. Programmed death ligand 1 (PD-L1) is a negative regulator of T cell function and is frequently expressed by solid tumors. By engaging programmed death 1 (PD-1) on activated T cells, P...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.03.023
更新日期:2012-07-01 00:00:00
abstract::ANCA associated vasculitis is a serious, very often recurrent disease that despite the current standard treatment with high-dose glucocorticoids and either cyclophosphamide or rituximab, patients have a nine-fold increased mortality risk in the first year compared with healthy controls, attributed to infections, vascu...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2019.06.018
更新日期:2019-08-01 00:00:00
abstract::In extension of the hypothesis that an amphipathic alpha helix of Ii (Phe146-Val164) bound to the foreign antigen-presenting site (desetope) of class II MHC molecules through hydrophobic amino acid residues (Phe146, Leu150, Leu153, Met157, Ile160, Val164) which were present in an axial strip along one side of the Ii h...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90068-x
更新日期:1987-10-01 00:00:00
abstract::A turbidimetric method for measuring the diameters of insoluble immune complexes, based on the wavelength dependence of their ability to scatter light, was developed. The method was validated by demonstrating that it gave experimental values for the diameters of polystyrene microspheres which were in good agreement wi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90074-l
更新日期:1993-05-01 00:00:00
abstract::Major histocompatibility complex (MHC) molecules present peptide antigens to T lymphocytes and initiate immune responses. The peptides loaded onto MHC class I or MHC class II molecules can be derived from cytosolic proteins, both self and foreign. A variety of cellular processes, including endocytosis, vesicle traffic...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.05.009
更新日期:2018-07-01 00:00:00
abstract::Heparin and two dextran sulphate preparations with a low or high average molecular mass (M(r) 5000 and 5 x 10(5), respectively) enhanced binding of radioactively labelled complement factor H to the complement protein C3b, coupled to Sepharose 4B, maximally 2.5-4-fold within a polyanion concn range of 12.5-400 microgra...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90082-m
更新日期:1993-02-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) in patients and certain mouse models exhibits a strong sex bias. Additionally, in most patients, increased serum levels of type I interferon (IFN-α) are associated with severity of the disease. Because increased levels of B cell activating factor (BAFF) in SLE patients and mouse mode...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.06.013
更新日期:2013-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.02.021
更新日期:2019-05-01 00:00:00
abstract::In the present study, we show that the treatment of Epstein-Barr virus (EBV) latently infected Raji cells with TPA/SB caused the cell growth arrest. The Zta-positive cells were predominantly enriched in G0/G1 phase of cell cycle. When Zta expression reached a maximal level, a fraction of Zta expressing cell population...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.017
更新日期:2010-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90263-2
更新日期:1982-12-01 00:00:00
abstract::The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.10.020
更新日期:2009-01-01 00:00:00
abstract::Reduced expression of Erythrocyte Complement Receptor 1 (E-CR1) is envisaged to contribute significantly to the pathophysiology of systemic lupus erythematosus (SLE). We determined the levels of CR1 transcript in the neutrophils from 25 untreated patients with active SLE and 25 normal healthy individuals and, studied ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.07.300
更新日期:2007-03-01 00:00:00
abstract::Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.04.023
更新日期:2008-01-01 00:00:00
abstract::T-cell receptor (TCR) repertoire diversity, thymic output, clonal size and peripheral T-lymphocyte numbers largely depend on intra-thymic and post-thymic T-lymphocyte proliferation. However, quantitative insight into thymocyte and T-lymphocyte proliferation is still lacking. We developed a new TCRG-based TCR excision ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.08.019
更新日期:2013-03-01 00:00:00
abstract::T cells play a crucial role in the pathogenesis of most autoimmune disorders. However, target antigens and pathomechanisms leading to human autoimmune diseases are still largely unknown. Cross-recognition of T cells between self and foreign antigens has been considered as a driving force in generating autoimmunity. He...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2003.11.012
更新日期:2004-02-01 00:00:00
abstract::Increasing evidence suggests that inflammation and apoptosis are involved in the development of acute myocardial ischemia (AMI). Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) have recently been identified as key mediators of inflammatory responses. The aim of this study was to explore the speci...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.06.013
更新日期:2016-08-01 00:00:00
abstract::C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.06.015
更新日期:2007-02-01 00:00:00
abstract::The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90181-i
更新日期:1991-09-01 00:00:00
abstract::While the antigenic variability is the major obstacle for developing vaccines against antigenically variable pathogens (AVPs) and cancer, this issue is not addressed adequately in current vaccine efforts. We developed a novel variable epitope library (VEL)-based vaccine strategy using immunogens carrying a mixture of ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.024
更新日期:2009-12-01 00:00:00