Abstract:
:Human promyelocytic leukemia (HL-60) cells were induced by 1,25-dihydroxyvitamin D3 (calcitriol) to differentiate and examined using a panel of monoclonal antibodies (MoAbs) and functional assays. Although morphologically and histochemically these cells appeared to be of the monocyte-macrophage phenotype, there was a decline in Fc receptors for IgGl and no induction of class II HLA antigens. There was, however, dramatic induction of the antigen detected by the myeloid-specific MoAb AML-2-23. These data suggest that the phenotypic changes induced by calcitriol in HL-60 cells are consistent with myelomonocytic differentiation in that the resultant cells possess characteristics of both monocytes (morphology, non-specific esterase staining, high levels of AML-2-23 reactivity) and granulocytes (PMN 29 binding, decreased Fc receptors for IgGl, absence of class II HLA antigens). Perhaps more important, the ability of calcitriol-treated cells to perform antibody-dependent cellular cytotoxicity and phagocytosis was markedly augmented. Lysis of antibody-coated erythrocytes by HL-60 cells increased from 5% in controls to 30-35% with calcitriol treatment for 4 days. This enhanced effector cell function was seen despite a decline in Fc receptors measured by cytofluorography. These data suggest that calcitriol may be involved in both differential and functional activation of myeloid cells.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Rigby WF,Shen L,Ball ED,Fanger MWdoi
10.1016/0161-5890(85)90180-4subject
Has Abstractpub_date
1985-05-01 00:00:00pages
567-72issue
5eissn
0161-5890issn
1872-9142journal_volume
22pub_type
杂志文章abstract::The variables regions of three syngeneic anti-idiotypic antibodies (Ab2s) were cloned and sequenced. They are encoded by different VL genes, two are from different members of V kappa-Ox1 superfamily. The H chains are encoded by VH genes belonging to three different VH families, J558, Q52 and 7183. Together with a prev...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90045-y
更新日期:1992-09-01 00:00:00
abstract::The fine regulation of NF-kappaB activity is crucial for both resting and stimulated cells and relies on complex balance between multiple activators and inhibitors. We report here that c-mip, a recently identified pleckstrin homology (PH) and leucine-rich repeat (LRR)-domain-containing protein, inactivates GSKbeta and...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.034
更新日期:2009-02-01 00:00:00
abstract::The immunoglobulin JHC mu intron was cloned from genomic DNA of a VHa3 rabbit and a 1257 bp sequence which contains conserved enhancer and splice sites was determined. From positions 315 to 1257, there is approximately 72 and 67% similarity to available sequences of man and mouse, respectively (counting gaps as single...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90119-3
更新日期:1989-10-01 00:00:00
abstract::Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90030-2
更新日期:1994-10-01 00:00:00
abstract::The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(01)00022-0
更新日期:2000-12-01 00:00:00
abstract::TRIMCyp generated by retrotransposition of a cyclophilin A inserting into TRIM5 locus, has been identified in owl monkey and most of Old World monkeys (OWM). Owl monkey TRIMCyp (omTRIMCyp) inhibits HIV-1 infection by direct interaction with viral capsid and indirect innate immune induction, whereas most of TRIMCyps fr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.08.001
更新日期:2018-09-01 00:00:00
abstract::The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90192-4
更新日期:1982-11-01 00:00:00
abstract::Chemokine effects on leukocyte infiltration into the central nervous system (CNS) are key events in the inflammatory processes of neuroimmunologic and neuroinfectious diseases. Because, chemokines may play important roles in proliferation and differentiation of brain cells and in the initiation and progression of CNS ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00019-6
更新日期:2002-05-01 00:00:00
abstract::Johne's disease is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratubercolosis (MAP) which affects ruminants worldwide and has a significant economic impact. MAP has also been associated with human Crohn's disease, although this connection is not well established. MAP is highly adapted for ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.017
更新日期:2017-11-01 00:00:00
abstract::The purpose of the present work was to determine the ability of BgTX peptides, corresponding to the various loops and exposed regions of alpha-bungarotoxin (BgTX) and representing regions that are recognized by B and/or T cells, to stimulate protective immunity in mice against in vivo challenge with BgTX. The BgTX LD5...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(96)00014-4
更新日期:1996-05-01 00:00:00
abstract::Mycobacterium tuberculosis (M.tb) infects 8 million and kills 2.2 million people each year worldwide. M.tb modulates the immune response of the infected individual. Empirically, suppressor carbohydrates (SC) produced by CD8+ T cells in response to M.tb were found to induce a T helper 2 response rather than a protectiv...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.022
更新日期:2008-03-01 00:00:00
abstract::Triptolide has been used extensively in China for the treatment of autoimmune diseases and tumor for many centuries. Nevertheless, little is known about its exact immunosuppressive and anti-inflammatory properties. Increasing recognition of the importance of renal tubular epithelial cells (TECs) in renal diseases rais...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.026
更新日期:2006-03-01 00:00:00
abstract::Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.018
更新日期:2010-04-01 00:00:00
abstract::Human complement components C6 and C7 have been purified and characterized. Component C6 is a single-chain plasma protein of mol. wt 104,800 containing 3.8% carbohydrate and it has alanine as the amino terminal residue. Component C7 is a single-chain plasma protein of mol. wt 92,400 containing 6.4% carbohydrate and it...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90189-4
更新日期:1982-11-01 00:00:00
abstract::Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90038-x
更新日期:1983-09-01 00:00:00
abstract::Peptide gpMBP72-85, containing amino acids 72-85 of guinea pig myelin basic protein is commonly used to induce experimental autoimmune encephalomyelitis in Lewis rats. The N-terminal glutamine in this peptide can cyclize to pyroglutamic acid, leading to loss of the first MHC anchor for binding to MHC class II. Acetyla...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2003.10.015
更新日期:2004-02-01 00:00:00
abstract::This report demonstrates that a marker of human embryonic endoderm and embryonal carcinoma cells recognized by a hybridoma antibody FC 10.2, involves Type 1 blood group chains with the sequence Gal beta 1 leads to 3G1cNAc beta 1 leads to 3Gal beta 1 leads to 4G1c. This conclusion has been reached from antigenic analys...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90005-6
更新日期:1983-06-01 00:00:00
abstract::The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.10.020
更新日期:2009-01-01 00:00:00
abstract::We considered the possibility that the greater the distance between an immune receptor V and J, the more likely the V usage. Such a hypothesis is supported by results from mouse experiments. And, such a hypothesis is consistent with the fundamental nature of recombination and genomic distance: the further the distance...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.10.010
更新日期:2019-12-01 00:00:00
abstract::Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90068-x
更新日期:1994-04-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.013
更新日期:2018-09-01 00:00:00
abstract::Asthma is a complex condition in which exposure to environmental antigens induces inflammatory reactions in the airway characterized by activation of mast cells and eosinophils. Mast cells are known to be the main effector cells in eliciting IgE-mediated allergic response. These cells secrete various substances that p...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00070-6
更新日期:2002-09-01 00:00:00
abstract::Autoimmunity is fundamentally a continuously evolving process. The autoimmune responses shift, drift and diversify with time not only to other epitopes in the original antigen but also to other related and sometimes to unrelated antigens. We have described a form of immune diversification--reciprocal T-B epitope sprea...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2003.11.029
更新日期:2004-02-01 00:00:00
abstract::A microchemical assay for phosphorus was applied to the measurement of DNA in immune complexes formed with monoclonal or serum anti-DNA autoantibodies and DNA of varying size and conformation. Two monoclonal antibodies were produced by hybridomas derived from spleen cells of autoimmune MRL-lpr/lpr mice and were purifi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90065-3
更新日期:1985-12-01 00:00:00
abstract::REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversifica...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.09.017
更新日期:2006-04-01 00:00:00
abstract::Sepsis-induced inflammatory damage is a crucial cause of acute kidney injury (AKI), and AKI is an ecumenical fearful complication in approximately half of patients with sepsis. CCAAT/enhancer-binding protein β (C/EBPβ) plays roles in regulating acute phase responses and inflammation. However, the role and mechanism of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.023
更新日期:2020-11-01 00:00:00
abstract::Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic activity, and key ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.04.025
更新日期:2007-02-01 00:00:00
abstract::Infectious salmon anaemia virus (ISAV) is a marine orthomyxovirus of significant interest not only as a cause of a fatal disease of farmed Atlantic salmon resulting in severe economic losses to the aquaculture industry, but also as the only poikilothermic orthomyxovirus. ISAV targets vascular endothelial cells and mac...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.06.015
更新日期:2009-09-01 00:00:00
abstract::The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90126-k
更新日期:1990-10-01 00:00:00
abstract::CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00055-8
更新日期:1998-06-01 00:00:00