Abstract：
:CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H3 and 5C6, by immunization of hamsters with purified CD1 protein. These antibodies recognize CD1-transfected cells and have no reactivity to cells isolated from CD1-/- mice. Both antibodies precipitate the 52 kDa heavy chain and 12 kDa beta2m from thymocytes and splenocytes by radio-immunoprecipitation. Deglycosylation of CD1 reduces molecular mass of the heavy chain by 7.5 kDa, which can be detected by 3H3 but not 5C6. 3H3 and 5C6 detect surface CD1 expression on cells from the thymus, spleen, lymph node and bone marrow, but not on intestinal epithelial cells. Developmentally, CD1 is expressed on thymocytes prior to TCR rearrangement and remains constant throughout thymic development. CD1 is expressed early in the fetal liver (day 14) and remains expressed in hepatocytes postnatally. These data support evidence of a role for CD1 in the selection and/or expansion of NK1- T cells of both thymic origin and extrathymic origin. Unlike classical class I molecules, murine CD1 levels are not affected by IFN-gamma, but like human CD1b can be up-regulated by IL-4 and GM-CSF although only moderately. Similar to human CD1b, murine CD1 is found by immunofluorescence microscopy on the cell surface, and in various intracellular vesicles, including early and late endosomes. Localization in endocytic compartments indicates that murine CD1 may be capable of binding endocytosed antigens.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Mandal M,Chen XR,Alegre ML,Chiu NM,Chen YH,Castaño AR,Wang CRdoi
10.1016/s0161-5890(98)00055-8subject
Has Abstractpub_date
1998-06-01 00:00:00pages
525-36issue
9eissn
0161-5890issn
1872-9142pii
S0161589098000558journal_volume
35pub_type
杂志文章abstract：:The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the NADPH oxidase complex, a multicomponent enzyme system that initiates a cascade of reactive oxygen species that play a critical role in innate immunity and vascular physiology. Epitope-mapped, monoclonal antibodies (mAb) that recognize...
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.molimm.2008.02.022
更新日期：2008-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.05.007
更新日期：2015-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2014.10.006
更新日期：2015-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.013
更新日期：2011-07-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2006-05-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2020-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.12.015
更新日期：2006-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(96)00034-x
更新日期：1996-06-01 00:00:00
