Abstract：
:A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived from mice undergoing chronic GVHD. The frequencies of successful fusion events correlate well with the number of activated B-cells used in the fusions, so that it is reasonable to conclude that the hybridoma collections reflect the activated B-cell repertoires in the different animals. The frequencies of hybridomas producing autoantibodies as well as their specificities for self-antigens, were not significantly different between the different collections of hybridomas. Moreover, no difference in VH gene family expression was found in the different collections of autoantibody producing hybridomas. So, the activated autoreactive B-cell repertoires in GVHF1 mice and in normal mice is similar. In contrast to the normal activated autoreactive B-cell repertoires, which make predominantly IgM antibodies, the GVH-activated autoreactive B-cells make predominantly antibodies of the IgG class. Therefore, we conclude that T-cell mediated graft versus host activation does not generally lead to selective expansion of autoreactive B-cells, but appears to play a crucial role in the switch from IgM to IgG production.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Rolink AG,Thalmann P,Berger C,Radaszkiewicz T,Melchers Fdoi
10.1016/0161-5890(88)90159-9subject
Has Abstractpub_date
1988-11-01 00:00:00pages
1217-22issue
11eissn
0161-5890issn
1872-9142journal_volume
25pub_type
杂志文章abstract：:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...
journal_title：Molecular immunology
pub_type： 杂志文章
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abstract：:We report that the protein tyrosine phosphatase PTP-PEST is expressed in resting human and mouse CD4(+) and CD8(+) T cells, but not in Jurkat T leukemia cells, and that PTP-PEST protein, but not mRNA, was dramatically downregulated in CD4(+) and CD8(+) primary human T cells upon T cell activation. This was also true i...
journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.019
更新日期：2018-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1987-12-01 00:00:00
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更新日期：1982-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90131-3
更新日期：1987-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.024
更新日期：2010-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2020-04-16 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(89)90101-6
更新日期：1989-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90072-0
更新日期：1985-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.008
更新日期：2014-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.022
更新日期：2007-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1994-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1998-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1985-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2008-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2009-09-01 00:00:00
