Abstract：
:Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found that the presence of AIM2, but not of the other inflammasome components Nod-like receptor (NLR) NLRP3 or NLRC4, specifically limited the transcription of the pro-inflammatory cytokines interleukin (IL)-6, IP-10, and tumor necrosis factor (TNF)-α in HL-1 mouse cardiomyocytes stimulated with IFN-γ and lipopolysaccharides (LPS). Similarly, AIM2 reduced pro-inflammatory cytokine transcription in primary mouse neonatal cardiomyocytes (MNC), but not in primary mouse neonatal cardiac fibroblasts (MNF). Interestingly, AIM2-dependent reduction of pro-inflammatory cytokines in cardiomyocytes was independent of Caspase-1. Mechanistically, AIM2 reduced pro-inflammatory cytokine transcription in cardiomyocytes by interacting with and inhibiting the phosphorylation of STAT1. In AIM2-depleted cardiomyocytes, increased STAT1 phosphorylation enhanced the NF-κB pathway by promoting NF-κB p65 phosphorylation and acetylation. These results show for the first time that AIM2 plays an important anti-inflammatory, yet inflammasome-independent function in cardiomyocytes. Our findings will help to further understand how the various heart cell types differently react to inflammatory stimuli.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Furrer A,Hottiger MO,Valaperti Adoi
10.1016/j.molimm.2016.04.009subject
Has Abstractpub_date
2016-06-01 00:00:00pages
47-58eissn
0161-5890issn
1872-9142pii
S0161-5890(16)30059-1journal_volume
74pub_type
杂志文章abstract：:The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. Soluble and membrane-bound complement regulators protect cells and tissues from unintended comp...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
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journal_title：Molecular immunology
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00019-6
更新日期：2002-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00100-7
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pub_type： 杂志文章
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更新日期：1994-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1998-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.05.007
更新日期：2012-10-01 00:00:00
