当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Multiple transcripts of the murine immunoglobulin epsilon membrane locus are generated by alternative splicing and differential usage of two polyadenylation sites.

Multiple transcripts of the murine immunoglobulin epsilon membrane locus are generated by alternative splicing and differential usage of two polyadenylation sites.

Abstract：

:The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon chain transcripts. The two poly(A) sites of the mouse membrane transcripts were identified in the present study by RACE-PCR analysis. The first poly(A) site is located 743 nt downstream from the beginning of the second membrane exon (M2) and contains the same non-consensus AGTAAA signal sequence as the single poly(A) site of the human membrane transcripts. The second poly(A) site is located almost 500nt further downstream and is characterized by an AAGAAA hexamer. This poly(A) site contains a (G+T) rich element downstream to the site of cleavage and polyadenylation and is preferentially utilized by the membrane epsilon transcripts. Additional diversity of epsilon transcripts is generated by alternative splicing between the last constant region exon (CH4) and the two membrane exons (M1 and M2). The alternatively spliced transcripts include two variants that skip the first membrane exon and encode epsilon heavy chains that lack the transmembrane domain. The third variant is generated by splicing to an internal site in M2 and codes for a membrane isoform that is 10 amino acids shorter in the cytoplasmic domain than the classical membrane IgE. Although little amino-acid sequence homology exists between the murine epsilon chain isoforms and their human counterparts, the pattern of splicing is rather conserved between the two species.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Anand S,Batista FD,Tkach T,Efremov DG,Burrone OR

doi

10.1016/s0161-5890(96)00110-1

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

175-83

issue

2

eissn

0161-5890

issn

1872-9142

pii

S0161589096001101

journal_volume

34

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • Dmu expression causes enrichment of MZ B cells, but is non permissive for B cell maturation in Rag2-/- mice even if combined with Bcl-2.

    abstract：:Rearrangements in reading frame 2 promote the expression of a truncated heavy chain, the Dmu protein. Dmu can assemble into a pre-B cell receptor like complex that appears to induce a subset of signals elicited by full length mu, but cannot promote the pro-B to pre-B cell transition of Rag-/- B cells. In order to dete...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.09.020

    authors： Wikström I,Bergqvist I,Holmberg D,Forssell J

    更新日期：2006-03-01 00:00:00

  • Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries.

    abstract：:Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.06.437

    authors： Goodchild SA,Dooley H,Schoepp RJ,Flajnik M,Lonsdale SG

    更新日期：2011-09-01 00:00:00

  • DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune response.

    abstract：:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(03)00024-5

    authors： Ito K,Ito K,Shinohara N,Kato S

    更新日期：2003-05-01 00:00:00

  • Immunochemical studies of conjugates of isomaltosyl oligosaccharides to lipid: production and characterization of mouse hybridoma antibodies specific for stearyl-isomaltosyl oligosaccharides.

    abstract：:Twelve C57BL/6J hybridoma clones, 9, 2 and 1 from mice immunized with stearyl-isomaltotetraose, stearyl-isomaltopentaose and stearyl-isomaltohexaose respectively were characterized. Seven produced IgA and 5 IgM. The specificities and sizes of their combining sites were determined by quantitative precipitin and precipi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(85)90105-1

    authors： Lai E,Kabat EA

    更新日期：1985-09-01 00:00:00

  • Low level formation of potent catalytic IgG fragments mediated by disulfide bond instability.

    abstract：:A highly purified preparation of a monoclonal antibody to vasoactive intestinal peptide (VIP) was analysed by gel filtration. Three peaks of VIP hydrolysing activity were observed, corresponding to the 150 kDa tetramer IgG, 80 kDa dimer of the heavy and light chains (H-L dimer) and 25 kDa L chain monomer. The hydrolyt...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(96)00021-1

    authors： Li L,Sun M,Gao QS,Paul S

    更新日期：1996-05-01 00:00:00

  • Analysis of the structural diversity of monoclonal antibodies to cyclosporine.

    abstract：:The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90126-k

    authors： Schmitter D,Poch O,Zeder G,Heinrich GF,Kocher HP,Quesniaux VF,Van Regenmortel MH

    更新日期：1990-10-01 00:00:00

  • Mouse IgG antibodies have subclass associated affinity differences.

    abstract：:Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of I...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90062-7

    authors： Sarvas HO,Seppälä IJ,Tähtinen T,Péterfy F,Mäkelä O

    更新日期：1983-03-01 00:00:00

  • Mangiferin suppresses allergic asthma symptoms by decreased Th9 and Th17 responses and increased Treg response.

    abstract：:Mangiferin is the major bioactive ingredient in the leaves of Mangifera indica L., Aqueous extract of such leaves have been traditionally used as an indigenous remedy for respiratory diseases including cough and asthma in Traditional Chinese Medicine. Mangiferin was shown to exert its anti-asthmatic effect by modulati...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.07.025

    authors： Yun C,Chang M,Hou G,Lan T,Yuan H,Su Z,Zhu D,Liang W,Li Q,Zhu H,Zhang J,Lu Y,Deng J,Guo H

    更新日期：2019-10-01 00:00:00

  • Induction of costimulatory molecules B7-1 and B7-2 in murine B cells. the CBA/N mouse reveals a role for Bruton's tyrosine kinase in CD40-mediated B7 induction.

    abstract：:The binding of CD40 ligand on activated T cells to CD40 on resting B cells induces the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The induction of B7 molecules by CD40 ligand-CD40 interaction represents a critical step in rendering B cells competent for antigen presentation. The CBA/N mouse has...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(96)00005-3

    authors： Goldstein MD,Debenedette MA,Hollenbaugh D,Watts TH

    更新日期：1996-04-01 00:00:00

  • Effect of reduction and alkylation on structure and function of rabbit IgG antibody--II. Effects on classical pathway C3 convertase formation.

    abstract：:Anaerobic reduction of purified rabbit IgG antibody (Ab) with 1.5 moles of dithiothreitol per mole of Ab at pH 8.0, followed by alkylation, cleaves 39% of the inter-heavy-chain (H-H) disulfide (SS) bonds. This treatment has the following effects on the ability of the Ab to activate the classical pathway of complement....

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(84)90092-0

    authors： Johnson BA,Hoffmann LG

    更新日期：1984-01-01 00:00:00

  • The expanding role of innate lymphoid cells and their T-cell counterparts in gastrointestinal cancers.

    abstract：:Innate lymphoid cells (ILCs) contribute to the regulation of gastrointestinal (GI) homeostasis. Over the past 15 years, there has been a large effort to dissect the mechanisms required for GI homeostasis, with a major focus on different immune cell populations and the cytokines that they produce. In contrast to T-help...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2017.11.013

    authors： Fung KY,Nguyen PM,Putoczki T

    更新日期：2019-06-01 00:00:00

  • Production of spliced peptides by the proteasome.

    abstract：:CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments ...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2018.03.030

    authors： Vigneron N,Stroobant V,Ferrari V,Abi Habib J,Van den Eynde BJ

    更新日期：2019-09-01 00:00:00

  • Immune evasion by acquisition of complement inhibitors: the mould Aspergillus binds both factor H and C4b binding protein.

    abstract：:Pathogenic fungi represent a major threat particularly to immunocompromised hosts, leading to severe, and often lethal, systemic opportunistic infections. Although the impaired immune status of the host is clearly the most important factor leading to disease, virulence factors of the fungus also play a role. Factor H ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.08.011

    authors： Vogl G,Lesiak I,Jensen DB,Perkhofer S,Eck R,Speth C,Lass-Flörl C,Zipfel PF,Blom AM,Dierich MP,Würzner R

    更新日期：2008-03-01 00:00:00

  • Mucosal innate immune response to intragastric infection by Salmonella enterica serovar Choleraesuis.

    abstract：:The innate immune response is critical to enteric disease resistance and the induction of mucosal adaptive immunity. In mucosae of the small intestine, Peyer's patches play a central role in immune surveillance and sampling of bacteria by specialized M cells. The innate immune response to Salmonella enterica serovar C...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.011

    authors： Hyland KA,Kohrt L,Vulchanova L,Murtaugh MP

    更新日期：2006-04-01 00:00:00

  • A monoclonal antibody recognizing a conserved epitope in a group of phospholipases A2.

    abstract：:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whe...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90055-5

    authors： Mollier P,Chwetzoff S,Ménez A

    更新日期：1990-01-01 00:00:00

  • Differential association of beta2-microglobulin mutants with MHC class I heavy chains and structural analysis demonstrate allele-specific interactions.

    abstract：:Dynamic interactions between major histocompatibility complex (MHC) class I heavy chains and beta2-microglobulin (beta2m) play a critical role in their stability on the cell surface, and their ability to present peptide antigens to CD8+ T-cells. A cursory review of protein sequence homologies and three-dimensional cry...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(99)00077-2

    authors： Shields MJ,Hodgson W,Ribaudo RK

    更新日期：1999-06-01 00:00:00

  • Processing of DR1-restricted determinants from the fusion protein of measles virus following two distinct pathways.

    abstract：:A panel of human T cell clones specific for measles virus was characterized and among them fusion protein-specific, DR1-and DP-restricted T cell clones were selected to study the processing and presentation of determinants borne by a viral membrane protein. Using two independent methods to assess the activation of T c...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(95)00148-4

    authors： Demotz S,Péléraux A

    更新日期：1996-03-01 00:00:00

  • Novel putative protein kinase clones from a rat large granular lymphocyte tumor cell line.

    abstract：:Protein kinases have been implicated in a number of regulatory mechanisms including signal transduction in many cells. To address the possibility that the large granular lymphocyte (LGL) also uses one or more unique protein kinases for LGL functions, an efficient method was developed to obtain partial cDNA clones for ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(91)90153-b

    authors： Yue CC

    更新日期：1991-04-01 00:00:00

  • The effect of alternate substrates and substrate concentration on the antibody-mediated inhibition of horseradish peroxidase.

    abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90169-4

    authors： Clark SK Jr,Conroy JM,Harris PJ

    更新日期：1983-12-01 00:00:00

  • Topology of the membrane-bound form of complement protein C9 probed by glycosylation mapping, anti-peptide antibody binding, and disulfide modification.

    abstract：:The two N-linked oligosaccharides in native human C9 were deleted by site-specific mutagenesis. This aglycosyl-C9 did not differ from its native form in hemolytic and bactericidal activity. A new N-glycosylation site (K311N/E313T) was introduced into the turn of a helix-turn-helix [HTH] fold that had been postulated t...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.01.013

    authors： Rossi V,Wang Y,Esser AF

    更新日期：2010-04-01 00:00:00

  • Molecular interactions of the CTLA-4 cytoplasmic region with the phosphoinositide 3-kinase SH2 domains.

    abstract：:During T-cell regulation, T-cell receptors and CD28 lead to signaling activation, while T-lymphocyte antigen 4 (CTLA-4) is known to lead to downregulation, similar to programmed cell death-1 (PD-1). In the cytoplasmic tails of CD28 and CTLA-4, phosphoinositide 3-kinase (PI3K) binds to the consensus sequence including ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.12.002

    authors： Iiyama M,Numoto N,Ogawa S,Kuroda M,Morii H,Abe R,Ito N,Oda M

    更新日期：2020-12-29 00:00:00

  • Comparative analysis of human and mouse 3' Igh regulatory regions identifies distinctive structural features.

    abstract：:Immunoglobulin heavy chain (Igh) locus rearrangements are controlled in part by an approximately 30 b complex 3' regulatory region located 3' of C alpha: this region contains several enhancers. We report here the comparison of the genomic sequences of the 3' regulatory region and further downstream sequences from mous...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2004.09.006

    authors： Sepulveda MA,Garrett FE,Price-Whelan A,Birshtein BK

    更新日期：2005-03-01 00:00:00

  • Mechanism of inhibition of lipopolysaccharide-induced interferon-β production by 2-aminopurine.

    abstract：:2-Aminopurine (2-AP) is widely used as an inhibitor for double stranded RNA-dependent protein kinase (PKR). Previously, we reported that 2-AP inhibits Toll-like receptor (TLR) ligand-induced nitric oxide production through the prevention of interferon (IFN)-β production. In this study, we investigated the mechanisms f...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.06.008

    authors： Sugiyama T,Gotou T,Moriyama K,Kajiura N,Hasegawa T,Tomida J,Takahashi K,Komatsu T,Ueda H,Sato K,Tokoro S,Neri P,Mori H

    更新日期：2012-10-01 00:00:00

  • Comparison of vasoactive intestinal peptide-mediated protein phosphorylation in human lymphoblasts and colonic epithelial cells.

    abstract：:Vasoactive intestinal peptide (VIP) induces phosphorylation of a basic 38,000 mol. wt protein in a human lymphoblastic cell line (Molt 4b) and a human colon carcinoma cell line (HT29). In both cell types, VIP interacts with specific high affinity receptors to activate adenylate cyclase and cAMP-dependent protein kinas...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90010-2

    authors： O'Dorisio MS,Campolito LB

    更新日期：1989-06-01 00:00:00

  • Characterization of Xenopus laevis complement factor I structure--conservation of modular structure except for an unusual insert not present in human factor I.

    abstract：:Factor I (C3b/C4b inactivator) is a regulatory protein of the classical and alternative complement pathways. In this paper, we report the sequence of Xenopus factor I cDNA and the deduced protein structure. The basic structure of human preprofactor I, NH2-heavy chain-cleavage peptide-light chain-COOH, is conserved in ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90040-i

    authors： Kunnath-Muglia LM,Chang GH,Sim RB,Day AJ,Ezekowitz RA

    更新日期：1993-10-01 00:00:00

  • Junctional diversity prevents negative selection of an antigen-specific T cell repertoire.

    abstract：:Endogenous mouse mammary tumor proviruses (MMTV; Mtv loci) deletes Vbeta6 expressing T cells in the thymus of Mtv-7(+) DBA/2 (H2(d)) mice through negative selection. We found that in Mtv-7(-) BALB/c (H2(d)) mice, Vbeta6 is a dominant V gene used in T cell responses to an 18 amino acid long peptide antigen: EYKEYAEYAEY...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.11.006

    authors： Kilgannon P,Novak Z,Fotedar A,Singh B

    更新日期：2010-02-01 00:00:00

  • Interaction of C3 and C3b with immunoglobulin G.

    abstract：:Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90076-7

    authors： Kulics J,Rajnavölgyi E,Füst G,Gergely J

    更新日期：1983-08-01 00:00:00

  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma.

    abstract：:The purpose of this study was to evaluate the effects of GATA-6 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control (PBS), ovalbumin (OVA)-induced asthma group (OVA), OVA+ s...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.05.017

    authors： Fang P,Shi HY,Wu XM,Zhang YH,Zhong YJ,Deng WJ,Zhang YP,Xie M

    更新日期：2016-07-01 00:00:00

  • MiR-143-3p controls TGF-β1-induced cell proliferation and extracellular matrix production in airway smooth muscle via negative regulation of the nuclear factor of activated T cells 1.

    abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracell...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.09.004

    authors： Cheng W,Yan K,Xie LY,Chen F,Yu HC,Huang YX,Dang CX

    更新日期：2016-10-01 00:00:00

  • Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis.

    abstract：:The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.09.016

    authors： Chen Y,Duan R,Li X,Li K,Liang J,Liu C,Qiu H,Xiao Y,Jing H,Wang X

    更新日期：2015-12-01 00:00:00