当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Mouse IgG antibodies have subclass associated affinity differences.

Mouse IgG antibodies have subclass associated affinity differences.

Abstract：

:Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of IgG varied from 5.1 to 8.6 mg/ml in the pools of immune sera and was 3.0 mg/ml in one normal serum tested. One half of this was IgG1, ca. 20% of IgG2a and IgG2b each, and 10% IgG3 in the pools of BALB/c sera. IgG2a and IgG3 could not be separated from C57BL sera (due to allotype b), but their combined share of IgG appears to be higher than in BALB/c. Immune sera contained 0.5-1.6 mg/ml of anti-NIP antibodies. Of this 90-98% was IgG1 and the remainder was split between the other subclasses. Up to one half of the protein in the IgG1 fraction was anti-NIP antibody. This surprising finding was confirmed by demonstrating that nearly 50% of the u.v.-light absorption was specifically removed by a NIP-immunosorbent. Subclass-associated affinity-differences were observed. IgG1 anti-NIP had a greater average affinity than IgG2a anti-NIP antibodies. The difference was ca. 1.5-fold when the equilibrium dialysis was focusing on the high-affinity bracket of the total population (concentration of free hapten 16-200 nM). At higher hapten concentrations the trend was the same but the data are fewer. Antibodies in subclasses IgG2b and IgG3 appear to share the lower affinity of IgG2a.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Sarvas HO,Seppälä IJ,Tähtinen T,Péterfy F,Mäkelä O

doi

10.1016/0161-5890(83)90062-7

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

239-46

issue

3

eissn

0161-5890

issn

1872-9142

journal_volume

20

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • Identification of a linear B-cell epitope in the catalytic domain of bothropasin, a metalloproteinase from Bothrops jararaca snake venom.

    abstract：:Bothropasin is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca venom, the snake responsible for most bites in Southeastern Brazil. SVMPs, such as bothropasin, are involved in the main bothropic envenoming symptoms, which include hemorrhage, inflammation, necrosis and blood coagulation deficie...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.10.019

    authors： Molina Molina DA,Guerra-Duarte C,Naves de Souza DL,Costal-Oliveira F,Ávila GR,Soccol VT,Machado-de-Ávila RA,Chávez-Olórtegui C

    更新日期：2018-12-01 00:00:00

  • MOTS-c peptide increases survival and decreases bacterial load in mice infected with MRSA.

    abstract：:Sepsis is a life-threatening disease characterized by uncontrolled inflammatory responses upon pathogen infections, especially for the antibiotic-resistant strains, such as Methicillin-resistant S. aureus (MRSA). Here we demonstrated that a Mitochondria-derived peptide (MOTS-c) could significantly improve the survival...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.10.017

    authors： Zhai D,Ye Z,Jiang Y,Xu C,Ruan B,Yang Y,Lei X,Xiang A,Lu H,Zhu Z,Yan Z,Wei D,Li Q,Wang L,Lu Z

    更新日期：2017-12-01 00:00:00

  • Effect of C1-INH on ischemia/reperfusion injury in a porcine limb ex vivo perfusion model.

    abstract：:Revascularization of an amputated limb within 4-6h is essential to avoid extensive ischemia/reperfusion (I/R) injury leading to vascular leakage, edema and tissue necrosis. I/R injury is a pathological inflammatory condition that occurs during reperfusion of an organ or tissue after prolonged ischemia. It is character...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.06.021

    authors： Abdelhafez MM,Shaw J,Sutter D,Schnider J,Banz Y,Jenni H,Voegelin E,Constantinescu MA,Rieben R

    更新日期：2017-08-01 00:00:00

  • Lipoteichoic acid from Lactobacillus plantarum inhibits Pam2CSK4-induced IL-8 production in human intestinal epithelial cells.

    abstract：:Lactobacilli are probiotic bacteria that are considered to be beneficial in the gastrointestinal tract of humans. Although lactobacilli are well known to alleviate intestinal inflammation, the molecular basis of this phenomenon is poorly understood. In this study, we investigated the effect of Lactobacillus plantarum ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.11.014

    authors： Noh SY,Kang SS,Yun CH,Han SH

    更新日期：2015-03-01 00:00:00

  • Structural comparison of murine T-cell (B151K12)-derived T-cell-replacing factor (IL-5) with rIL-5: dimer formation is essential for the expression of biological activity.

    abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90158-v

    authors： Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

    更新日期：1990-09-01 00:00:00

  • HMGB1 induced inflammatory effect is blocked by CRISPLD2 via MiR155 in hepatic fibrogenesis.

    abstract：:A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.10.018

    authors： Zhang H,Liu Z,Liu S

    更新日期：2016-01-01 00:00:00

  • Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT and LvDorsal to activate the promoter of white spot syndrome virus immediate-early gene ie1.

    abstract：:White spot syndrome virus (WSSV) has caused great economic damage to shrimp aquaculture. Previous studies have shown that WSSV successfully usurps the immunity system of the host for its own gene regulation. To investigate the role of shrimp high mobility group box (HMGB) proteins in WSSV gene regulation, two Litopena...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.11.020

    authors： Chen YH,Jia XT,Huang XD,Zhang S,Li M,Xie JF,Weng SP,He JG

    更新日期：2011-02-01 00:00:00

  • Regulation of NKG2D-ligand cell surface expression by intracellular calcium after HDAC-inhibitor treatment.

    abstract：:In this study we demonstrate that histone deacetylase (HDAC)-inhibitor mediated cell surface expression of the structural different NKG2D-ligands MICA/B and ULBP2 is calcium-dependent. Treatment with the calcium chelator EGTA inhibited constitutive as well as HDAC-inhibitor induced MICA/B and ULBP2 cell surface expres...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.08.011

    authors： Jensen H,Hagemann-Jensen M,Lauridsen F,Skov S

    更新日期：2013-03-01 00:00:00

  • Antigenic sites on the arginine-rich carboxyl-terminal domain of the capsid protein of hepatitis B virus distinct from hepatitis B core or e antigen.

    abstract：:The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90130-2

    authors： Machida A,Ohnuma H,Takai E,Tsuda F,Tanaka T,Naito M,Munekata E,Miyakawa Y,Mayumi M

    更新日期：1989-04-01 00:00:00

  • Sequence analysis of porcine immunoglobulin light chain cDNAs.

    abstract：:A porcine cDNA library was constructed using poly(A)+ RNA isolated from the spleen of an adult Minnesota miniature swine. Screening the library with antisera specific for porcine immunoglobulin light chains resulted in the selection and isolation of two recombinant clones, PLC18 and PLC3, which encode for kappa and la...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(91)90051-k

    authors： Lammers BM,Beaman KD,Kim YB

    更新日期：1991-08-01 00:00:00

  • APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis.

    abstract：:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.11.002

    authors： Chang Y,Jia X,Sun X,Xu S,Wu Y,Zhang L,Wei W

    更新日期：2015-03-01 00:00:00

  • Mannose-binding lectin is produced by vaginal epithelial cells and its level in the vaginal fluid is influenced by progesterone.

    abstract：:Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents co...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.07.016

    authors： Bulla R,De Seta F,Radillo O,Agostinis C,Durigutto P,Pellis V,De Santo D,Crovella S,Tedesco F

    更新日期：2010-11-01 00:00:00

  • Acute arsenic exposure induces inflammatory responses and CD4+ T cell subpopulations differentiation in spleen and thymus with the involvement of MAPK, NF-kB, and Nrf2.

    abstract：:Increasing lines of evidence indicate that arsenic may be associated with immune related problems, but its detailed effects on immune organs are poorly understood. The objective of this study was to explore inflammatory responses and T cell differentiation of arsenic exposure in spleen and thymus. Female C57BL/6 mice ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.12.005

    authors： Duan X,Gao S,Li J,Wu L,Zhang Y,Li W,Zhao L,Chen J,Yang S,Sun G,Li B

    更新日期：2017-01-01 00:00:00

  • Induction of costimulatory molecules B7-1 and B7-2 in murine B cells. the CBA/N mouse reveals a role for Bruton's tyrosine kinase in CD40-mediated B7 induction.

    abstract：:The binding of CD40 ligand on activated T cells to CD40 on resting B cells induces the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The induction of B7 molecules by CD40 ligand-CD40 interaction represents a critical step in rendering B cells competent for antigen presentation. The CBA/N mouse has...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(96)00005-3

    authors： Goldstein MD,Debenedette MA,Hollenbaugh D,Watts TH

    更新日期：1996-04-01 00:00:00

  • Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.

    abstract：:Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study had several previously ident...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.02.013

    authors： Mohlin FC,Nilsson SC,Levart TK,Golubovic E,Rusai K,Müller-Sacherer T,Arbeiter K,Pállinger É,Szarvas N,Csuka D,Szilágyi Á,Villoutreix BO,Prohászka Z,Blom AM

    更新日期：2015-06-01 00:00:00

  • Molecular basis of C-reactive protein binding and modulation of complement activation by factor H-related protein 4.

    abstract：:C-reactive protein (CRP) is a pattern recognition molecule that binds several microbial and host ligands. Ligand-bound CRP activates the complement system via the classical pathway. Previously, we identified human complement factor H-related protein 4 (CFHR4), a member of the factor H protein family, as a CRP binding ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.12.005

    authors： Hebecker M,Okemefuna AI,Perkins SJ,Mihlan M,Huber-Lang M,Józsi M

    更新日期：2010-03-01 00:00:00

  • Detection of an antigen on the inner surface of Rh negative erythrocytes which binds anti-D IgG.

    abstract：:Previous investigation have demonstrated the presence of the Rho(D) antigen in Rh negative erythrocytes. The intact Rh negative cell, however, does not bind anti-D IgG. Presently we have shown that an anti-D binding antigen resides on the cytoplasmic surface of Rh negative erythrocyte membranes. Unsealed Rh negative m...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90091-3

    authors： Evans JP,Brown PJ,Sinor LT,Tilzer LL,Beck M,Plapp FV

    更新日期：1983-05-01 00:00:00

  • Intracellular co-localization of SPLUNC1 protein with nanobacteria in nasopharyngeal carcinoma epithelia HNE1 cells depended on the bactericidal permeability increasing protein domain.

    abstract：:Epithelial surfaces constitute natural immunobarriers against environmental threats. These barriers are brimming with fluids that bind, transport, cleave or degrade bacterial cells and their endotoxic by-products. Saliva and the airway surface-lining fluid (ASL) comprise the important fluid constituents. Short palate,...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.021

    authors： Zhou HD,Li GY,Yang YX,Li XL,Sheng SR,Zhang WL,Zhao J

    更新日期：2006-04-01 00:00:00

  • Pre-existing CD19-independent GL7(-) Breg cells are expanded during inflammation and in mice with lupus-like disease.

    abstract：:Interleukin 10 (IL-10)-producing regulatory B-cells (Bregs) suppress inflammatory responses that mediate autoimmune diseases. However, it is unknown whether Bregs derive from a pre-existing dedicated B-cell lineage or if any B-cell can differentiate into Bregs in response to BCR or TLR activation. GL7(+) B-cells are a...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.01.011

    authors： Wang X,Wei Y,Xiao H,Liu X,Zhang Y,Han G,Chen G,Hou C,Zhang L,Ma N,Shen B,Li Y,Egwuagu CE,Wang R

    更新日期：2016-03-01 00:00:00

  • Dysregulation of T cell immunoglobulin and mucin domain 3 (TIM-3) signaling in peripheral immune cells is associated with immune dysfunction in autistic children.

    abstract：:Evidence suggests that immune dysregulation is associated with autism spectrum disorder (ASD). T cell immunoglobulin and mucin domain-3 (TIM-3) has a critical role in several inflammatory disorders; however, the role of TIM-3 signaling has not been demonstrated in ASD. In the present study, we assessed the role of TIM...

    journal_title：Molecular immunology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.molimm.2018.12.020

    authors： Ahmad SF,Ansari MA,Nadeem A,Bakheet SA,Al-Ayadhi LY,Alotaibi MR,Alhoshani AR,Alshammari MA,Attia SM

    更新日期：2019-02-01 00:00:00

  • Natural DNP-binding immunoglobulins and antibody multispecificity.

    abstract：:Anti-DNP activity was found in normal human immunoglobulins with average affinities (10(4)-10(6) l/mole) and frequencies (15-2%) that correlate well with those expected from multispecific binding sites. ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(84)90143-3

    authors： Ortega E,Kostovetzky M,Larralde C

    更新日期：1984-10-01 00:00:00

  • Genetic analysis of the complement factor H related 5 gene in haemolytic uraemic syndrome.

    abstract：:Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.08.004

    authors： Monteferrante G,Brioschi S,Caprioli J,Pianetti G,Bettinaglio P,Bresin E,Remuzzi G,Noris M

    更新日期：2007-03-01 00:00:00

  • DC-STAMP interacts with ER-resident transcription factor LUMAN which becomes activated during DC maturation.

    abstract：:Dendritic cells (DCs) are the professional antigen-presenting cells (APC) which efficiently prime the immune response or induce tolerance. We recently identified Dendritic Cell Specific TrAnsMembrane Protein (DC-STAMP), a novel 470 amino acid protein preferentially expressed by dendritic cells. Previously we demonstra...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.04.019

    authors： Eleveld-Trancikova D,Sanecka A,van Hout-Kuijer MA,Looman MW,Hendriks IA,Jansen BJ,Adema GJ

    更新日期：2010-07-01 00:00:00

  • Mutational pressure makes HIV1 gp120 linear B-cell epitopes shorter and may lead to their disappearance.

    abstract：:We showed that nucleotide sequences coding for linear B-cell epitopes of human immunodeficiency virus type 1 (HIV1) gp120 protein are enriched with codons containing cytosine and guanine in their first and second codon positions. Guanine and cytosine are the most mutable nucleotides in HIV1 genes (due to APOBEC3 and A...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.01.006

    authors： Khrustalev VV

    更新日期：2010-04-01 00:00:00

  • Generation of a comprehensive panel of crustacean allergens from the North Sea Shrimp Crangon crangon.

    abstract：BACKGROUND:Published data on crustacean allergens are incomplete. The identification of tropomyosin (TM), arginine kinase (AK), sarcoplasmic Ca-binding protein (SCP) and myosin light chain (MLC) as shrimp allergens are all important contributions but additional allergens are required for the development of a complete s...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.06.216

    authors： Bauermeister K,Wangorsch A,Garoffo LP,Reuter A,Conti A,Taylor SL,Lidholm J,Dewitt AM,Enrique E,Vieths S,Holzhauser T,Ballmer-Weber B,Reese G

    更新日期：2011-09-01 00:00:00

  • A priori delineation of a peptide which mimics a discontinuous antigenic determinant.

    abstract：:A technique was developed for identifying peptides with high affinity for a given antibody. By testing a monoclonal antibody directed against a discontinuous antigenic determinant on foot-and-mouth disease virus, peptides mimicking the determinant were identified even though the tertiary structure of the proteins comp...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(86)90081-7

    authors： Geysen HM,Rodda SJ,Mason TJ

    更新日期：1986-07-01 00:00:00

  • A murine antibody to Shigella dysenteriae type 1 employs V-genes that contain a rearranged codon for the lambda light chain.

    abstract：:The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding an...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(95)00072-m

    authors： Miller CE,Huppi K,Siwarski D,Karpas A,Newman A,Mainhart C,Glaudemans CP

    更新日期：1995-06-01 00:00:00

  • Thermodynamic analysis of additivity between the heavy and light chains in affinity maturation of an antibody.

    abstract：:In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...

    journal_title：Molecular immunology

    pub_type： 评论,信件

    doi：10.1016/j.molimm.2007.07.007

    authors： Kang J,Warren AS

    更新日期：2008-01-01 00:00:00

  • Structural analysis of a phosphorylcholine-binding antibody which exhibits a unique carrier specificity for Trichinella spiralis.

    abstract：:A phosphorylcholine (PC)-binding IgG (Mab2) antibody produced by a hybridoma derived from a BALB/c mouse which had been immunized against Trichinella spiralis was found to bind to the immunizing antigen (TSC) but not to other PC-associated antigens such as pneumococcal antigen (PNC) and PC-conjugated ovalbumin (PC-OVA...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(94)90106-6

    authors： Lim PL,Leung DT,Chui YL,Ma CH

    更新日期：1994-10-01 00:00:00

  • More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release.

    abstract：:The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(94)90122-8

    authors： Daibata M,Xu M,Humphreys RE,Reyes VE

    更新日期：1994-03-01 00:00:00